Dan Shao
Columbia University
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Publication
Featured researches published by Dan Shao.
ACS Applied Materials & Interfaces | 2016
Dan Shao; Xin Zhang; Wenliang Liu; Fan Zhang; Xiao Zheng; Ping Qiao; Jing Li; Wen-Fei Dong; Li Chen
A facile and cheap strategy was used to fabricate the novel Janus silver-mesoporous silica nanoparticles with excellent SPR and mesoporous properties for simultaneous SERS imaging and pH-responsive drug release, leading to the efficient cancer theranostic with less toxic effects.
ACS Applied Materials & Interfaces | 2014
Dan Shao; Jing Li; Xuanang Xiao; Ming Zhang; Yue Pan; Shuo Li; Zheng Wang; Xin Zhang; Huilin Zheng; Xuewen Zhang; Li Chen
Exploring intracellular behavior of suicide gene is significant for improving the efficacy and safety of herpes simplex virus thymidine kinase gene/ganciclovir (HSV-TK/GCV) system in cancer therapy. Molecular imaging represents a powerful tool to understand gene transportation and function dynamics. In this work, we reported a quantum-dot-based technique for revealing the procedure of HSV-TK/GCV suicide gene therapy by constructing covalent linkage between near-infrared fluorescent quantum dots (QDs) and TK gene. This stable QD labeling did not influence either the QDs fluorescence or the biological activity of TK gene. Furthermore, we visualized and dynamically traced the intracellular behavior antitumor effect of TK gene in vitro and in vivo. It is demonstrated that TK gene was shuttled to the nucleus after a-24 h treatment; at that time the single dose of GCV administration exerts the gradually increasing lethal effect until to 72 h. Real-time tracing the formation of hepatocellular carcinoma treated with HSV-TK/GCV suicide gene system in vivo by QD-based NIR fluorescence imaging provides useful insight toward QD-based theranostics in future cancer therapy.
ACS Nano | 2017
Zheng Wang; Dan Shao; Zhimin Chang; Mengmeng Lu; Ying-Shuai Wang; Juan Yue; Dian Yang; Mingqiang Li; Qiaobing Xu; Wen-Fei Dong
There is a pressing need to develop nanoplatforms that integrate multimodal therapeutics to improve treatment responses and prolong the survival of patients with unresectable hepatocellular carcinoma (HCC). Mesoporous silica-coated gold nanomaterials have emerged as a novel multifunctional platform combining tunable surface plasmon resonance and mesoporous properties that exhibit multimodality properties in cancer theranostics. However, their reduced radiation-absorption efficiency and limited surface area hinder their further radiochemotherapeutic applications. To address these issues, we designed Janus-structured gold-mesoporous silica nanoparticles using a modified sol-gel method. This multifunctional theranostic nanoplatform was subsequently modified via the conjugation of folic acid for enhanced HCC targeting and internalization. The loaded anticancer agent doxorubicin can be released from the mesopores in a pH-responsive manner, facilitating selective and safe chemotherapy. Additionally, the combination of chemotherapy and radiotherapy induced synergistic anticancer effects in vitro and exhibited remarkable inhibition of tumor growth in vivo along with significantly reduced systematic toxicity. Additionally, the Janus NPs acted as targeted computed tomography (CT)-imaging agents for HCC diagnosis. Given their better performance in chemoradiotherapy and CT imaging as compared with that of their core-shell counterparts, this new nanoplatform designed with dual functionalities provides a promising strategy for unresectable HCC theranostics.
RSC Advances | 2016
Zheng Wang; Ying-Shuai Wang; Mengmeng Lu; Li Li; Yi Zhang; Xiao Zheng; Dan Shao; Jing Li; Wen-Fei Dong
The combination of chemotherapy and photothermotherapy is emerging as a promising strategy for the treatment of liver cancer as a result of its synergistic efficacy. A safe and efficient drug-delivery system is highly desirable to ensure that the anticancer drug and photothermal agent can be simultaneously delivered to a tumor region to exert their synergistic effect with reduced side-effects. Uniform Janus Au–mesoporous silica nanoparticles with superior surface plasmon resonance properties and a high surface area were designed to integrate a high drug-loading capacity, pH-responsive properties, and a superior photothermal effect into a single carrier. The ability of the Janus nanoparticles loaded with doxorubicin to combine local specific chemotherapy with external near-infrared photothermotherapy significantly improved the therapeutic efficacy against liver cancer cells while exerting less toxicity on normal liver cells. Hence the reported doxorubicin-loaded Janus NPs may be promising therapeutic agents for efficacious and safe treatment of liver cancer.
Chemical Biology & Drug Design | 2017
Zheng Wang; Ying-Shuai Wang; Zhimin Chang; Li Li; Yi Zhang; Mengmeng Lu; Xiao Zheng; Mingqiang Li; Dan Shao; Jing Li; Li Chen; Wen-Fei Dong
Berberine, an bioactive isoquinolin alkaloid from traditional Chinese herbs, is considered to be a promising agent based on its remarkable activity against hepatocellular carcinoma. However, the clinical application of this nature compound had been hampered owing to its properties such as poor aqueous solubility, low gastrointestinal absorption, and reduced bioavailability. Therefore, we developed Janus magnetic mesoporous silica nanoparticles (Fe3O4‐mSiO2 NPs) consisting of a Fe3O4 head for magnetic targeting and a mesoporous SiO2 body for berberine delivery. A pH‐sensitive group was introduced on the surface of mesoporous silica for berberine loading to develop a tumor microenvironment‐responsive nanocarrier, which exhibited uniform morphology, good superparamagnetic properties, high drug‐loading amounts, superior endocytic ability, and low cytotoxicity. Berberine‐loaded Fe3O4‐mSiO2 NPs exerted extraordinarily high specificity for hepatocellular carcinoma cells, which was due to the pH‐responsive berberine release, as well as higher endocytosis capacity in hepatocellular carcinoma cells rather than normal liver cells. More importantly, an external magnetic field could significantly improve antitumor activity of Ber‐loaded Fe3O4‐mSiO2 NPs through enhancing berberine internalization. Taken together, our results suggest that Janus nanocarriers driven by the magnetic field may provide an effective and safe way to facilitate clinical use of berberine against hepatocellular carcinoma.
Advanced Materials | 2018
Dan Shao; Mingqiang Li; Zheng Wang; Xiao Zheng; Yeh-Hsing Lao; Zhimin Chang; Fan Zhang; Mengmeng Lu; Juan Yue; Hanze Hu; Huize Yan; Li Chen; Wen-Fei Dong; Kam W. Leong
Controlled delivery of protein therapeutics remains a challenge. Here, the inclusion of diselenide-bond-containing organosilica moieties into the framework of silica to fabricate biodegradable mesoporous silica nanoparticles (MSNs) with oxidative and redox dual-responsiveness is reported. These diselenide-bridged MSNs can encapsulate cytotoxic RNase A into the 8-10 nm internal pores via electrostatic interaction and release the payload via a matrix-degradation controlled mechanism upon exposure to oxidative or redox conditions. After surface cloaking with cancer-cell-derived membrane fragments, these bioinspired RNase A-loaded MSNs exhibit homologous targeting and immune-invasion characteristics inherited from the source cancer cells. The efficient in vitro and in vivo anti-cancer performance, which includes increased blood circulation time and enhanced tumor accumulation along with low toxicity, suggests that these cell-membrane-coated, dual-responsive degradable MSNs represent a promising platform for the delivery of bio-macromolecules such as protein and nucleic acid therapeutics.
ACS Applied Materials & Interfaces | 2017
Zheng Wang; Zhimin Chang; Mengmeng Lu; Dan Shao; Juan Yue; Dian Yang; Mingqiang Li; Wen-Fei Dong
Stimuli-triggered nanoplatforms have become attractive candidates for combined strategies for advanced liver cancer treatment. In this study, we designed a light-responsive nanoplatform with folic acid-targeting properties to surmount the poor aqueous stability and photostability of indocyanine green (ICG). In this Janus nanostructure, ICG was released on-demand from mesoporous silica compartments in response to near-infrared (NIR) irradiation, exhibiting predominant properties to convert light to heat in the cytoplasm to kill liver cancer cells. Importantly, the silver ions released from the silver compartment that were triggered by light could induce efficient chemotherapy to supplement photothermal therapy. Under NIR irradiation, ICG-loaded Janus nanoplatforms exhibited synergistic therapeutic capabilities both in vitro and in vivo compared with free ICG and ICG-loaded mesoporous silica nanoparticles themselves. Hence, our Janus nanoplatform could integrate ICG-based photothermal therapy and silver ion-based chemotherapy in a cascade manner, which might provide an efficient and safe strategy for combined liver cancer therapy.
ACS Applied Materials & Interfaces | 2018
Zhimin Chang; Zheng Wang; Dan Shao; Juan Yue; Hao Xing; Li Li; Mingfeng Ge; Mingqiang Li; Huize Yan; Hanze Hu; Qiaobing Xu; Wen-Fei Dong
Magnetic mesoporous silica nanoparticles (M-MSNs) are attractive candidates for the immunomagnetic isolation and detection of circulating tumor cells (CTCs). Understanding of the interactions between the effects of the shape of M-MSNs and CTCs is crucial to maximize the binding capacity and capture efficiency as well as to facilitate the sensitivity and efficiency of detection. In this work, fluorescent M-MSNs were rationally designed with sphere and rod morphologies while retaining their robust fluorescence and uniform surface functionality. After conjugation with the antibody of epithelial cell adhesion molecule (EpCAM), both of the differently shaped M-MSNs-EpCAM obtained achieved efficient enrichment of CTCs and fluorescent-based detection. Importantly, rodlike M-MSNs exhibited faster immunomagnetic isolation as well as better performance in the isolation and detection of CTCs in spiked cells and real clinical blood samples than those of their spherelike counterparts. Our results showed that shape engineering contributes positively toward immunomagnetic isolation, which might open new avenues to the rational design of magnetic-fluorescent nanoprobes for the sensitive and efficient isolation and detection of CTCs.
RSC Advances | 2017
Zhimin Chang; Zheng Wang; Mengmeng Lu; Mingqiang Li; Li Li; Yi Zhang; Dan Shao; Wen-Fei Dong
Magnetic Janus mesoporous silica nanoparticles (MSNs) with CTAB-loading and amino-functionalization were prepared through a one-pot synthesis strategy. Janus MSNs exhibit outstanding bacterial capture and separation performance, enabling the highly efficient elimination of both Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus, illustrating their application potential in biomedicine.
Colloids and Surfaces B: Biointerfaces | 2017
Zhi-min Chang; Zheng Wang; Mengmeng Lu; Dan Shao; Juan Yue; Dian Yang; Mingqiang Li; Wen-Fei Dong
In this study, we exploited a one-pot method to fabricate cetyltrimethylammonium bromide (CTAB)-loaded Janus silver mesoporous silica nanoparticles (Janus Ag-MSNs@CTAB). These bullet-like nanoparticles had a silver head (80nm in diameter) attached to a mesoporous silica stick (200-300nm in length). The CTAB-loaded nanobullets exhibited a marked affinity for the bacterial cell surface and the simultaneously sustained release behavior of CTAB and silver ions. The minimum inhibitory concentrations (MIC) of Janus Ag-MSNs@CTAB were determined to be 10μg/mL and 20μg/mL for E. coli and S. Aureus, respectively. Importantly, Janus Ag-MSNs@CTAB provided a single-particle nanoplatform with a synergistic effect against both Gram-positive and Gram-negative bacteria. A thorough investigation indicated that CTAB induces a dramatic loss of bacterial membrane integrity, which facilitated the internalization of silver. This report described an efficient and convenient method of synthesizing Janus silver mesoporous silica nanoparticles, and these nanobullets show promising potential in biomedical applications.