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Publication
Featured researches published by Danas Baniulis.
Plant Genetic Resources | 2012
Kristiina Antonius; Saila Karhu; Hedi Kaldmäe; G. Lacis; R. Rugenius; Danas Baniulis; Audrius Sasnauskas; Erik Schulte; A. Kuras; M. Korbin; Å. Gunnarsson; Gun Werlemark; Darius Ryliškis; T. Todam-Andersen; L. Kokk; K. Järve
The purpose of the study was to support the selection process of the most valuable currant and gooseberry accessions cultivated in Northern Europe, in order to establish a decentralized core collection and, following the selection, to ensure sufficient genetic diversity in the selected collection. Molecular analyses of the material from nine project partners were run at seven different laboratories. The results were first analysed for each partner separately, and then combined to ensure sufficient genetic diversity in the core collection.
Zemdirbyste-agriculture | 2016
Rytis Rugienius; Lina Šnipaitiene; Gražina Stanienė; Jūratė Bronė Šikšnianienė; Perttu Haimi; Danas Baniulis; Birutė Frercks; Viktoras Pranckietis; Vanda Lukoševičiūtė; Vidmantas Stanys
Winter hardiness is one of the main traits affecting survival, productivity and cultivar choice for important horticultural plants in a temperate climatic zone. Given the complexity of the trait and the often fluctuating winter weather, extensive field testing over many years would be required to gain reliable results. Testing for cold tolerance under controlled conditions to grade genotypes has been used in several plant breeding programs to identify superior genotypes of different species. The aim of the present study was to investigate cold tolerance of microshoots of different genotypes of the genera Prunus and Fragaria after 7, 14, 28 and 56 days of cold acclimation, by measuring ion leakage and evaluating the critical temperature (CT50) after freezing in vitro. Genotypes of Rosacea family demonstrated distinct patterns of CT50 change during cold acclimation. Cold acclimation for 56 days decreased the CT50 value by 1.3–2.0°C for Prunus microshots and by 0.8–2.1°C for Fragaria microshoots in comparison to nonacclimated plants. The results of cold acclimation and freezing treatments of Rosacea family plants in vitro show that for maximal cold hardiness, acclimation for 56 days or longer is required. Sour cherry (Prunus cerasus) cv. ‘Orkolija’, garden strawberry (Fragaria × ananassa) cv. ‘Melody’, Virginia strawberry (F. virginiana) and musk strawberry (F. moschata) were the most winterhardy after 56 days’ cold acclimation. The data obtained in the study can be used to improve breeding and cryopreservation technologies of Rosacea plants, and lay the foundation for identification of genes responsible for efficient cold acclimation and low temperature tolerance.
Biologija | 2008
Danas Baniulis; Julius Liobikas; Dalia Gelvonauskienė; Audrius Sasnauskas; Gintautas Vaitiekaitis; Vidmantas Stanys
Thaumatin-II is an intense sweet tasting protein isolated from fruits of Thaumatococcus daniel lii. Thaumatin is a member of the pathogenesis-related protein family referred to as thaumatinlike proteins (TLPs). Expression of thaumatin-II in plants was associated with an enhanced resistance against pathogens. In addition, thaumatin was used to provide the sweet taste quality to plant products. Thus, TLP proteins have a potential application for improving plant stress resistance and taste qualities using the recombinant DNA technology. However, several members of the TLP family were characterized as food allergens. The allergenic properties of thaumatinII have not been characterized. In this study, a putative allergenicity of thaumatin-II was established using computational analysis of sequence similarity with known human allergens. Antigenicity analysis and multiple sequence alignment of related TLP sequences identified six putative allergenic epitopes. The residues Thr12, Leu74, Gln133 and Thr161 of thaumatin-II or equivalent residues of other TLPs were proposed as a target for mutagenesis aimed to develop protein isoforms with reduced allergenicity.
Journal of Plant Physiology | 2017
Perttu Haimi; Jurgita Vinskienė; Inga Stepulaitienė; Danas Baniulis; Gražina Stanienė; Jūratė Bronė Šikšnianienė; Rytis Rugienius
Important crop plants of Rosaceae family are often damaged during winter due to the lack of acclimation and cold hardiness. One of the cellular responses of plants to cold stress is the accumulation of dehydrin proteins. We studied the expression of dehydrins in several Rosaceae species during low temperature treatment in vitro. Microshoots of Pyrus communis, Malus×domestica, Fragaria vesca, Fragaria×ananassa, Prunus cerasus and Prunus avium cultivars were grown in low temperature conditions. Genotype -specific accumulation of dehydrins was detected by immunoblot analysis of the extracted proteins. Untargeted difference gel electrophoresis of Malus x domestica microshoots revealed an extensive accumulation of three dehydrins. In a protein phosphatase assay, MdDHN2 and MdDHN4, but not MdDHN6 proteins were found to be extensively phosphorylated. In terms of the amount of protein synthesized, dehydrins are a major protein-level adaptation mechanism to low temperature in M. x domestica. In addition to dehydrins, the induction of proteins involved in the response for oxidative stress were observed. Additionally, a Xero2 -like dehydrin of F. vesca was detected by difference gel electrophoresis and identified by nano LC-MS/MS.
Plant Methods | 2013
Sidona Sikorskaite; Minna-Liisa Rajamäki; Danas Baniulis; Vidmantas Stanys; Jari P. T. Valkonen
Zemdirbyste-agriculture | 2012
Sidona Sikorskaite; Dalia Gelvonauskiene; Vidmantas Stanys; Danas Baniulis
Zemdirbyste-agriculture | 2016
Inga Miliute; Odeta Buzaite; Dalia Gelvonauskiene; Audrius Sasnauskas; Vidmantas Stanys; Danas Baniulis
Zemdirbyste-agriculture | 2012
Danas Baniulis; Inga Stepulaitiene; Vanda Lukoseviciute; Asta Blazyte; Vidmantas Stanys; Rytis Rugienius; Audrius Sasnauskas
Horticulturae | 2016
Audrius Sasnauskas; Vidmantas Bendokas; Rasa Karklelienė; Danguolė Juškevičienė; Tadeušas Šikšnianas; Dalia Gelvonauskienė; Rytis Rugienius; Danas Baniulis; Sidona Sikorskaitė-Gudžiūnienė; Ingrida Mažeikienė; Audrius Radzevičius; Nijolė Maročkienė; Eugenijus Dambrauskas; Vidmantas Stanys
Biologija | 2010
Danas Baniulis; Julius Liobikas; Subhash Mohan Agarwal; Birutė Frercks; Gintautas Vaitiekaitis; Vidmantas Stanys