Danfeng Yang
Academy of Military Medical Sciences
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Journal of Applied Toxicology | 2009
Hui Yang; Chao Liu; Danfeng Yang; Hua-Shan Zhang; Zhuge Xi
Although the biological effects of some nanomaterials have already been assessed, information on toxicity and possible mechanisms of various particle types are insufficient. Moreover, the role of particle properties in the toxic reaction remains to be fully understood. In this paper, we aimed to explore the interrelationship between particle size, shape, chemical composition and toxicological effects of four typical nanomaterials with comparable properties: carbon black (CB), single wall carbon nanotube, silicon dioxide (SiO2) and zinc dioxide (ZnO) nanoparticles. We investigated the cytotoxicity, genotoxicity and oxidative effects of particles on primary mouse embryo fibroblast cells. As observed in the methyl thiazolyl tetrazolium (MTT) and water‐soluble tetrazolium (WST) assays, ZnO induced much greater cytotoxicity than non‐metal nanoparticles. This was significantly in accordance with intracellular oxidative stress levels measured by glutathione depletion, malondialdehyde production, superoxide dismutase inhibition as well as reactive oxygen species generation. The results indicated that oxidative stress may be a key route in inducing the cytotoxicity of nanoparticles. Compared with ZnO nanoparticles, carbon nanotubes were moderately cytotoxic but induced more DNA damage determined by the comet assay. CB and SiO2 seemed to be less effective. The comparative analysis demonstrated that particle composition probably played a primary role in the cytotoxic effects of different nanoparticles. However, the potential genotoxicity might be mostly attributed to particle shape. Copyright
Biomedical and Environmental Sciences | 2009
Zhi-Qing Lin; Zhu-ge X; Danfeng Yang; Fu-Huan Chao; Hua-Shan Zhang; Wei Zhang; Huang-Liang Liu; Zai-Ming Yang; Rubao Sun
OBJECTIVE To investigate the oxidative damage to lung tissue and peripherial blood in PM2.5-treated rats. METHODS PM2.5 samples were collected using an auto-sampling instrument in summer and winter. Treated samples were endotracheally instilled into rats. Activity of reduced glutathione peroxidase (GSH-Px) and concentration of malondialdehyde (MDA) were used as oxidative damage biomarkers of lung tissue and peripheral blood detected with the biochemical method. DNA migration length (microm) and rate of tail were used as DNA damage biomarkers of lung tissue and peripheral blood detected with the biochemical method. RESULTS The activity of GSH-Px and the concentration of MDA in lung tissue significantly decreased after exposure to PM2.5 for 7-14 days. In peripheral blood, the concentration of MDA decreased, but the activity of GSH-Px increased 7 and 14 days after experiments. The two indicators had a dose-effect relation and similar changing tendency in lung tissue and peripheral blood. The DNA migration length (microm) and rate of tail in lung tissue and peripheral blood significantly increased 7 and 14 days after exposure to PM2.5. The two indicators had a dose-effect relation and similar changing tendency in lung tissue and peripheral blood. CONCLUSION PM2.5 has a definite oxidative effect on lung tissue and peripheral blood. The activity of GSH-Px and the concentration of MDA are valuable biomarkers of oxidative lung tissue damage induced by PM2.5. The DNA migration length (microm) and rate of tail are simple and valuable biomarkers of PM2.5-induced DNA damage in lung tissues and peripheral blood. The degree of DNA damage in peripheral blood can predict the degree of DNA damage in lung tissue.
Journal of Hazardous Materials | 2013
Huanliang Liu; Danfeng Yang; Honglian Yang; Huashan Zhang; Wei Zhang; Yanjun Fang; Zhiqing Lin; Lei Tian; Bencheng Lin; Jun Yan; Zhuge Xi
Atmospheric Environment | 2007
Rubao Sun; Zhu-Ge Xi; Fu-Huan Chao; Wei Zhang; Hua-Shan Zhang; Danfeng Yang
Atmospheric Environment | 2011
Wei Zhang; Tian Lei; Zhi-Qing Lin; Hua-Shan Zhang; Danfeng Yang; Zhu-Ge Xi; Jian-Hua Chen; Wei Wang
Biomedical and Environmental Sciences | 2005
Yang Yh; Zhu-Ge Xi; Fu-Huan Chao; Danfeng Yang
Biomedical and Environmental Sciences | 2005
Zhu-Ge Xi; Fu-Huan Chao; Danfeng Yang; Hua-Shan Zhang; Wei Zhang
Archive | 2010
Zhi-Qing Lin; Rubao Sun; Wei Zhang; Danfeng Yang; Hua-Shan Zhang; Zhuge Xi
Archive | 2010
Danfeng Yang; Huashan Zhang; Wei Zhang; Zhuge Xi
Archive | 2009
Zhuge Xi; Huashan Zhang; Wei Zhang; Guanxian Li; Danfeng Yang