Daniel A. Hammer
Applied Science Private University
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Featured researches published by Daniel A. Hammer.
Current Opinion in Colloid and Interface Science | 2000
Bohdana M. Discher; Daniel A. Hammer; Frank S. Bates; Dennis E. Discher
Abstract Vesicles can be made from various polymers, from modified biological to wholly synthetic, in media ranging from complex to neat organics and water. Although the canonical form of polymers that have the ability to assemble into encapsulating vesicles is an amphiphilic H–P (hydrophobic–polar) diblock, additional block copolymers from triblocks to pentablocks have also been shown to assemble into vesicles. Polydispersity seems to have a minimal effect, but solvent requirements may well depend on the proportion of H to P as well as the molecular weight. What is clear from all of the ultrastructural characterization done on polymer vesicles is that the ‘universal’ thickness of 3–4 nm, which is well-known for natural lipid vesicles, reflects no physical limitation on the amphiphilic assembly at the nano-scale. Emerging material characterizations emphasize the dominance of the two-dimensional H–P interface and the unnatural robustness possible in the responses of polymer membranes.
Nanoscale | 2013
Wei Qi; P. Peter Ghoroghchian; Guizhi Li; Daniel A. Hammer; Michael J. Therien
Nanoparticles formed from diblock copolymers of FDA approved PEO and PCL have generated considerable interest as in vivo drug delivery vehicles. Herein, we report the synthesis of the most extensive family PEO-b-PCL copolymers that vary over the largest range of number-average molecular weights (Mn: 3.6-57k), PEO weight fractions (fPEO: 0.08-0.33), and PEO chain lengths (0.75-5.8k) reported to date. These polymers were synthesized in order to establish the full range of aqueous phase behaviours of these diblock copolymers and to specifically identify formulations that were able to generate bilayered vesicles (polymersomes). Cryogenic transmission electron microscopy (cryo-TEM) was utilized in order to visualize the morphology of these structures upon aqueous self-assembly of dry polymer films. Nanoscale polymersomes were formed from PEO-b-PCL copolymers over a wide range of PEO weight fractions (fPEO: 0.14-0.27) and PEO molecular weights (0.75-3.8k) after extrusion of aqueous suspensions. Comparative morphology diagrams, which describe the nature of self-assembled structures as a function of diblock copolymer molecular weight and PEO weight fraction, show that in contrast to micron-scale polymersomes, which form only from a limited range of PEO-b-PCL diblock copolymer compositions, a multiplicity of PEO-b-PCL diblock copolymer compositions are able to give rise to nanoscale vesicles. These data underscore that PEO-b-PCL compositions that spontaneously form micron-sized polymersomes, as well as those that have previously been reported to form polymersomes via a cosolvent fabrication system, provide only limited insights into the distribution of PEO-b-PCL diblocks that give rise to nanoscale vesicles. The broad range of polymersome-forming PEO-b-PCL compositions described herein suggest the ability to construct extensive families of nanoscale vesicles of varied bilayer thickness, providing the ability to tune the timescales of vesicle degradation and encapsulant release based on the intended in vivo application.
Ultrasound in Medicine and Biology | 2018
Katherine W. Pulsipher; Daniel A. Hammer; Daeyeon Lee; Chandra M. Sehgal
Microbubbles interact with ultrasound in various ways to enable their applications in ultrasound imaging and diagnosis. To generate high contrast and maximize therapeutic efficacy, microbubbles of high uniformity are required. Microfluidic technology, which enables precise control of small volumes of fluid at the sub-millimeter scale, has provided a versatile platform on which to produce highly uniform microbubbles for potential applications in ultrasound imaging and diagnosis. Here, we describe fundamental microfluidic principles and the most common types of microfluidic devices used to produce sub-10 μm microbubbles, appropriate for biomedical ultrasound. Bubbles can be engineered for specific applications by tailoring the bubble size, inner gas and shell composition and by functionalizing for additional imaging modalities, therapeutics or targeting ligands. To translate the laboratory-scale discoveries to widespread clinical use of these microfluidic-based microbubbles, increased bubble production is needed. We present various strategies recently developed to improve scale-up. We conclude this review by describing some outstanding problems in the field and presenting areas for future use of microfluidics in ultrasound.
Small | 2018
Woo-Sik Jang; Hyun Ji Kim; Chen Gao; Daeyeon Lee; Daniel A. Hammer
Cell motility is central to processes such as wound healing, immune cell surveillance, and embryonic development. Motility requires the conversion of chemical to mechanical energy. An active area of research is to create motile particles, such as microswimmers, using catalytic and enzymatic reactions. Here, autonomous motion is demonstrated in adhesive polymer-based protocells by incorporating and harnessing the energy production of an enzymatic reaction. Biotinylated polymer vesicles that encapsulate catalase, an enzyme which converts hydrogen peroxide to water and oxygen, are prepared and these vesicles are adhered weakly to avidin-coated surfaces. Upon addition of hydrogen peroxide, which diffuses across the membrane, catalase activity generates a differential impulsive force that enables the breakage and reformation of biotin-avidin bonds, leading to diffusive vesicle motion resembling random motility. The random motility requires catalase, increases with the concentration of hydrogen peroxide, and needs biotin-avidin adhesion. Thus, a protocellular mimetic of a motile cell.
Macromolecules | 2006
P. Peter Ghoroghchian; Guizhi Li; Dalia H. Levine; Kevin P. Davis; Frank S. Bates; Daniel A. Hammer; Michael J. Therien
Archive | 2008
Joshua S. Katz; Dalia H. Levine; Daniel A. Hammer; Jason A. Burdick
Archive | 2007
Dennis E. Discher; Bohdana M. Discher; You-Yeon Won; James Lee; Daniel A. Hammer; Frank S. Bates
Archive | 2008
Guizhi Li; P. Peter Ghoroghchian; Wei Qi; Natalie A. Christian; Paul R. Frail; Daniel A. Hammer; Michael J. Therien
Archive | 2004
Daniel A. Hammer; Michael J. Therien; P. Peter Ghoroghchian
Archive | 2001
Dennis E. Discher; Harry Bermudez; Daniel A. Hammer; Frank S. Bates; Bohdana M. Discher