Daniel K. Y. Tan

Effect of high temperature on the reproductive development of chickpea genotypes under controlled environments

High temperature during the reproductive stage in chickpea (Cicer arietinum L.) is a major cause of yield loss. The objective of this research was to determine whether that variation can be explained by differences in anther and pollen development under heat stress: the effect of high temperature during the pre- and post-anthesis periods on pollen viability, pollen germination in a medium, pollen germination on the stigma, pollen tube growth and pod set in a heat-tolerant (ICCV 92944) and a heat-sensitive (ICC 5912) genotype was studied. The plants were evaluated under heat stress and non-heat stress conditions in controlled environments. High temperature stress (29/16°C to 40/25°C) was gradually applied at flowering to study pollen viability and stigma receptivity including flower production, pod set and seed number. This was compared with a non-stress treatment (27/16°C). The high temperatures reduced pod set by reducing pollen viability and pollen production per flower. The ICCV 92944 pollen was viable at 35/20°C (41% fertile) and at 40/25°C (13% fertile), whereas ICC 5912 pollen was completely sterile at 35/20°C with no in vitro germination and no germination on the stigma. However, the stigma of ICC 5912 remained receptive at 35/20°C and non-stressed pollen (27/16°C) germinated on it during reciprocal crossing. These data indicate that pollen grains were more sensitive to high temperature than the stigma in chickpea. High temperature also reduced pollen production per flower, % pollen germination, pod set and seed number.

Life cycle energy and greenhouse gas analysis for agave-derived bioethanol

The sustainability of large-scale biofuel production has recently been called into question in view of mounting concerns over the associated impact on land and water resources. As the most predominant biofuel today, ethanol produced from food crops such as corn in the US has been frequently criticised. Ethanol derived from cellulosic feedstocks is likely to overcome some of these drawbacks, but the production technology is yet to be commercialised. Sugarcane ethanol is the most efficient option in the short term, but its success in Brazil is difficult to replicate elsewhere. Agaves are attracting attention as potential ethanol feedstocks because of their many favourable characteristics such as high productivities and sugar content and their ability to grow in naturally water-limited environments. Here, we present the first life cycle energy and greenhouse gas (GHG) analysis for agave-derived ethanol. The results suggest that ethanol derived from agave is likely to be superior, or at least comparable, to that from corn, switchgrass and sugarcane in terms of energy and GHG balances, as well as in ethanol output and net GHG offset per unit land area. Our analysis highlights the promising opportunities for bioenergy production from agaves in arid or semi-arid regions with minimum pressure on food production and water resources.

High temperature tolerance in chickpea and its implications for plant improvement

Abstract. Chickpea (Cicer arietinum L.) is an important food legume and heat stress affects chickpea ontogeny over a range of environments. Generally, chickpea adapts to high temperatures through an escape mechanism. However, heat stress during reproductive development can cause significant yield loss. The most important effects on the reproductive phase that affect pod set, seed set and yield are: (1) flowering time, (2) asynchrony of male and female floral organ development, and (3) impairment of male and female floral organs. While this review emphasises the importance of high temperatures >30°C, the temperature range of 32–35°C during flowering also produces distinct effects on grain yield. Recent field screening at ICRISAT have identified several heat-tolerant germplasm, which can be used in breeding programs for improving heat tolerance in chickpea. Research on the impact of heat stress in chickpea is not extensive. This review describes the status of chickpea production, the effects of high temperature on chickpea, and the opportunities for genetic improvement of chickpea tolerance to high temperatures.

Agave as a biofuel feedstock in Australia

The opportunity for commercial production of Agave in Australia stems from the substantial carbohydrate and fibre content of Agave, the nature of carbohydrates stored, the pre‐existence of an Agave ethanol‐producing industry in Mexico, demand for biofuel feedstocks, impressive water‐use efficiencies of plants with the CAM pathway, and legislation mandating the ethanol content of fuel in Australia, where an estimated 748 ML will be required for blending with petrol by 2010–2011, compared with about 440 ML in 2009. Agave has potential as a crop for areas of seasonally limited rainfall in Australia, a judgement based upon desktop analyses and agronomic experience of growing Agave in Australia before 1915. Development of a viable Australian Agave farming system requires production be located in suitable regions, efficient propagation methods, mechanized production, and viable business plans at grower, processor and marketing levels. Growers and processors agree that Agave will not be grown commercially until plants are grown, maintained and harvested in Australia, product is produced and tested, and yield and risks are evaluated. To this end, Agave were imported into Australia from Mexico and a tissue culture propagation technique developed. A trial crop of Agave tequilana was planted in northern Queensland and CO2 exchange, nitrogen and water dynamics, carbohydrate content, and system inputs and outputs are being monitored. The experience will be used to fine‐tune the farming system, assess production costs and develop robust life‐cycle assessments. Processing of plants from trials will test harvesting and transport infrastructure and will provide material to processors for testing. Samples will be provided to potential producers of value‐added products. An Australian Agave industry should provide opportunities for stimulating agronomic, scientific and commercial exchange between Australia and Mexico. Successful integration of Agave into Australian agriculture will require a biofuels‐focussed breeding programme in collaboration with Mexican researchers.

Predicting broccoli development: II. Comparison and validation of thermal time models.

Models predicting broccoli ontogeny and maturity should ideally be precise and readily adopted by farmers and researchers. The objective of this study was to compare the predictive accuracy of thermal time models for three broccoli (Brassica oleracea L. var. italica Plenck) cultivars (‘Fiesta’, ‘Greenbelt’ and ‘Marathon’) from emergence to harvest maturity (Model 1), from emergence to floral initiation (Model 2), and from floral initiation to harvest maturity (Model 3). Comparisons were also made between Model 1 and Model 4 (Models 2 and 3 combined). Model 1 is useful when the timing of floral initiation is not known. When Model 1 was tested using independent data from 1983 to 1984 sowings of three cultivars (‘Premium Crop’, ‘Selection 160’ and ‘Selection 165A’), it predicted harvest maturity well. Prediction of floral initiation using Model 2 is useful for timing cultural practices, frost and heat avoidance. Where timing of floral initiation was recorded, predictions of harvest maturity were most precise using Model 3, since the variation which occurred from emergence to floral initiation was removed. The good predictions for Model 4 suggests that it would best predict the chronological duration from emergence to harvest maturity. # 2000 Published by Elsevier Science B.V.

Potential Suitability and Viability of Selected Biodiesel Crops in Australian Marginal Agricultural Lands Under Current and Future Climates

The potential environmental suitability and economic viability of growing two biodiesel crops in marginal regions of Australia were explored. Firstly, we used spatial analysis techniques to identify marginal agricultural regions suitable for growing pongam (Pongamia pinnata) and Indian mustard (Brassica juncea), and determined the base socioeconomic viability of investments for the production of biodiesel in the identified areas. Secondly, we used climate change projections (target years 2020 to 2070) from the Commonwealth Scientific, Industrial and Research Organization Mk3.0 global circulation model generated for two emission scenarios (A1B and A1FI) to determine the shift in potential areas for these crops. Under the climate change scenarios tested, the total area suitable for growing pongam between 2040 and 2070 is substantially different from the suitable area under current climate, indicating that long-term investments in this perennial tree crop may not be viable in all regions, especially in southern Australia. There is a greater variation in suitability projections for Indian mustard, although there is more flexibility for cropping options given that it is an annual crop. However, future economic viability is likely to depend on the ability to receive renewable energy certificates for both crops and, in the case of pongam, the certified emission reductions. Opportunities exist for sustainable pongam agroforestry to supply biodiesel to regional towns, cattle stations and mines in northern Australia.

Consequences of waterlogging in cotton and opportunities for mitigation of yield losses

Cotton is a major world crop that is notoriously susceptible to waterlogging damage, particularly when cultivated on fine-textured soils. However, damage is also exacerbated because of inadequate acclimation of roots to low oxygen levels, and secondary effects on shoots. Despite the commercial importance of cotton, very little has been published when compared with waterlogged cereals. This review provides a comprehensive view of the constraints on cotton in low-oxygen conditions, including absence of aerenchyma and the inadequacy of fermentation to overcome waterlogging damage. We emphasise the possibilities of improved tolerance through management practices, manipulation of hormone pathways and gene technologies to modify perception and response to low-oxygen environments.

Potential contribution by cotton roots to soil carbon stocks in irrigated Vertosols

The well-documented decline in soil organic carbon (SOC) stocks in Australian cotton (Gossypium hirsutum L.) growing Vertosols has been primarily analysed in terms of inputs from above-ground crop residues, with addition to soil C by root materials being little studied. Potential contribution by cotton roots to soil carbon stocks was evaluated between 2002 and 2008 in 2 ongoing long-term experiments near Narrabri, north-western New South Wales. Experiment 1 consisted of cotton monoculture sown either after conventional tillage or on permanent beds, and a cotton–wheat (Triticum aestivum L.) rotation on permanent beds; Experiment 2 consisted of 4 cotton-based rotation systems sown on permanent beds: cotton monoculture, cotton–vetch (Vicia villosa Roth.), cotton–wheat, and cotton–wheat–vetch. Roundup-Ready™ (genetically modified) cotton varieties were sown until 2005, and Bollgard™ II-Roundup Ready™-Flex™ varieties thereafter. Root growth in the surface 0.10 m was measured with the core-break method using 0.10-m-diameter cores. A subsample of these cores was used to evaluate relative root length and root C concentrations. Root growth in the 0.10–1.0 m depth was measured at 0.10-m depth intervals with a ‘Bartz’ BTC-2 minirhizotron video microscope and I-CAP image capture system (‘minirhizotron’). The video camera was inserted into clear, plastic acrylic minirhizotron tubes (50-mm-diameter) installed within each plot, 30° from the vertical. Root images were captured 4–5 times each season in 2 orientations, left and right side of each tube, adjacent to a furrow, at each time of measurement and the images analysed to estimate selected root growth indices. The indices evaluated were the length and number of live roots at each time of measurement, number of roots which changed length, number and length of roots which died (i.e. disappeared between times of measurement), new roots initiated between times of measurement, and net change in root numbers and length. These measurements were used to derive root C turnover between times of measurements, root C added to soil through intra-seasonal root death, C in roots remaining at end of season, and the sum of the last 2 indices: root C potentially available for addition to soil C stocks. Total seasonal cotton root C potentially available for addition to soil C stocks ranged between ~50 and 400 g/m2 (0.5 and 4 t/ha), with intra-seasonal root death contributing 25–70%. These values are ~10–60% of that contributed by above-ground crop residues. As soil organic carbon in irrigated Vertosols can range between 40 and 60 t/ha, it is unlikely that cotton roots will contribute significantly to soil carbon stocks in irrigated cotton farming systems. Seasonal root C was reduced by cotton monoculture, stress caused by high insect numbers, and sowing Bollgard II varieties; and increased by sowing non-Bollgard II varieties and wheat rotation crops. Permanent beds increased root C but leguminous rotation crops did not. Climatic factors such as cumulative day-degrees and seasonal rainfall were positively related to seasonal root C. Root C turnover was, in general, highest during later vegetative/early reproductive growth. Large variations in root C turnover and seasonal C indices occurred due to a combination of environmental, management and climatic factors.

Freeze-induced reduction of broccoli yield and quality

Summary. Sub-zero temperatures can result in freezing injury of broccoli (Brassica oleracea L. var. italica Plenck) plants and thereby reduce head yield and quality. In order to predict effects of frosts, it is desirable to know the stages of development at which broccoli plants are most susceptible to freezing injury. In this study, the effect of a range of sub-zero temperatures for a short period at different stages of crop development were assessed and quantified in terms of mortality, yield and quality of broccoli. Whole plants in pots or in the field were subjected to sub-zero temperature regimes from –1 to –19°C. Extracellular ice formation was achieved by reducing temperatures slowly, at –2°C per hour. The floral initiation stage was most sensitive to freezing injury, as yields (fresh and dry head weights) were significantly reduced at –1 and –3°C, and the shoot apices were killed at –5°C. There was no significant yield reduction when the inflorescence buttoning stage was treated at –1 and –3°C. Although shoot apices survived the –5°C treatment at buttoning, very poor quality heads of uneven bud size were produced as a result of arrested development. The lethal temperature for pot-grown broccoli was between –3 and –5°C, whereas the lethal temperature for field-grown broccoli was between –7 and –9°C. The difference was presumably due to variation in cold acclimation. Freezing injury can reduce broccoli head yield and quality and retard plant growth. With regard to yield and maturity prediction, crop development models based only on simple thermal time without restrictions will not apply if broccoli crops are frost damaged.

Understanding the molecular events underpinning cultivar differences in the physiological performance and heat tolerance of cotton (Gossypium hirsutum)

Diurnal or prolonged exposure to air temperatures above the thermal optimum for a plant can impair physiological performance and reduce crop yields. This study investigated the molecular response to heat stress of two high-yielding cotton (Gossypium hirsutum L.) cultivars with contrasting heat tolerance. Using global gene profiling, 575 of 21854 genes assayed were affected by heat stress, ~60% of which were induced. Genes encoding heat shock proteins, transcription factors and protein cleavage enzymes were induced, whereas genes encoding proteins associated with electron flow, photosynthesis, glycolysis, cell wall synthesis and secondary metabolism were generally repressed under heat stress. Cultivar differences for the expression profiles of a subset of heat-responsive genes analysed using quantitative PCR over a 7-h heat stress period were associated with expression level changes rather than the presence or absence of transcripts. Expression differences reflected previously determined differences for yield, photosynthesis, electron transport rate, quenching, membrane integrity and enzyme viability under growth cabinet and field-generated heat stress, and may explain cultivar differences in leaf-level heat tolerance. This study provides a platform for understanding the molecular changes associated with the physiological performance and heat tolerance of cotton cultivars that may aid breeding for improved performance in warm and hot field environments.