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Dive into the research topics where Daniel R. Rigsbee is active.

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Featured researches published by Daniel R. Rigsbee.


Pharmaceutical Research | 1997

The Stability of Insulin in Crystalline and Amorphous Solids: Observation of Greater Stability for the Amorphous Form

Michael J. Pikal; Daniel R. Rigsbee

AbstractPurpose. Generalizations based upon behavior of small molecules have established that a crystalline solid is generally much more stable toward chemical degradation than is the amorphous solid. This study examines the validity of this generalization for proteins using biosynthetic human insulin as the model protein. Methods. Amorphous insulin was prepared by freeze drying the supernate from a suspension of zinc insulin crystals adjusted to pH 7.1. Storage stability at 25°C and 40°C were compared for the freeze dried material, the dried suspended crystals, and the starting batch of crystals. Samples were equilibrated at selected relative humidities between zero and 75% to obtain samples at various water contents. Assays for dimer formation were performed by size exclusion HPLC and assays for deamidated product were carried out by reverse phase HPLC. Degradation was found to be linear in square root of time, and the slopes from % degradation vs. square root of time were used to define the rate constants for degradation. Differential scanning calorimetry (DSC) and Fourier-transform infrared spectroscopy (FTIR) were used to characterize the state of the protein in the solids. Results. As expected based upon previous results, the primary degradation pathways involve deamidation at the AsnA21 site and co-valent dimer formation, presumably involving the A-21 site. Contrary to expectations, amorphous insulin is far more stable than crystalline insulin under all conditions investigated. While increasing water content increases the rate of degradation of crystalline insulin, rate constants for degradation in the amorphous solid are essentially independent of water content up to the maximum water content studied (≈15%). Conclusions. Based upon the FTIR and DSC data, both crystalline and amorphous insulin retain some higher order structure when dried, but the secondary structure is significantly perturbed from that characteristic of the native solution state. However, neither DSC nor FTIR data provide a clear interpretation of the difference in stability between the amorphous and crystalline solids. The mechanism responsible for the superior stability of amorphous insulin remains obscure.


Journal of Pharmaceutical Sciences | 2008

Solid state chemistry of proteins: II. The correlation of storage stability of freeze‐dried human growth hormone (hGH) with structure and dynamics in the glassy solid

Michael J. Pikal; Daniel R. Rigsbee; Michael L. Roy; Dawn Galreath; Karl J. Kovach; Bingquan Wang; John F. Carpenter; Marcus T. Cicerone

This research presents storage stability of human growth hormone, hGH, in lyophilized di-saccharide formulations. Stability via HPLC assay was assessed at 40 and 50 degrees C. Structure of the protein in the solids was assessed by infrared spectroscopy. Molecular mobility was characterized by structural relaxation times estimated from DSC data and by measurement of atomic motion on a nanosecond time scale by neutron scattering. Very large stability differences were observed among the various formulations, with both chemical and aggregation stability showing the same qualitative trends with formulation. Near the T(g), T(g) appeared to be a relevant stability parameter, but for storage well below T(g), stability seems unrelated to T(g). Stability (chemical and aggregation) was weakly correlated with secondary structure of the protein, and there was a partial quantitative correlation between degradation rate and the structural relaxation time. However, at equivalent levels of disaccharide relative to protein, sucrose systems were about a factor of two more stable than trehalose formulations, but yet had greater mobility as measured by structural relaxation time. Secondary structure was equivalent in both formulations. Neutron scattering results documented greater suppression of fast dynamics by sucrose than by trehalose, suggesting that well below T(g), fast dynamics are important to stability.


Journal of Pharmaceutical Sciences | 2009

Solid state chemistry of proteins IV. what is the meaning of thermal denaturation in freeze dried proteins

Michael J. Pikal; Daniel R. Rigsbee; Michael J. Akers

This research addresses the thermodynamic significance of the denaturation endotherm observed during differential scanning calorimetry (DSC) scans of proteins in dry formulations, such as freeze dried solids. Human growth hormone formulations are the chosen representative examples. We employ observations of denaturation temperature, glass transition temperature, and the differences between estimated molecular mobilities to argue that unfolding is under partial thermodynamic control. Further, unfolding during a DSC scan is simulated using a three state kinetic model, which is a two state unfolding model followed by aggregation. Kramers-type rate constants are used, where the preexponential term is dominated by viscous forces. Simulation results are in qualitative agreement with experiment, and clearly show that while the denaturation endotherm is impacted by irreversibility, caused by nonzero scan rate and aggregation, the position of the endotherm peak is changed only slightly. Thus, the denaturation peak is a good approximation for the thermodynamic denaturation temperature. Using data for denaturation temperature, heat of denaturation, and heat capacity of denaturation, free energy versus temperature curves were calculated. We find that even formulations with added saccharides are thermodynamically unstable near ambient temperature; significant denaturation in the solid state is prevented by low mobility.


Journal of Pharmaceutical Sciences | 2002

Dynamics of Pharmaceutical Amorphous Solids: The Study of Enthalpy Relaxation by Isothermal Microcalorimetry

Jinsong Liu; Daniel R. Rigsbee; Carol Stotz; Michael J. Pikal


Journal of Pharmaceutical Sciences | 1998

Formation of isomorphic desolvates: Creating a molecular vacuum

Gregory A. Stephenson; Edward G. Groleau; Rita L. Kleemann; Wei Xu; Daniel R. Rigsbee


Journal of Pharmaceutical Sciences | 1998

Determination of the glass properties of D‐mannitol using sorbitol as an impurity

Lian Yu; Dinesh Mishra; Daniel R. Rigsbee


Macromolecules | 1993

Structural Elucidation of Soluble Polyelectrolyte-Micelle Complexes: Intra- vs Interpolymer Association

Jiulin Xia; Huiwen Zhang; Daniel R. Rigsbee; Paul L. Dubin; Tehseen Shaikh


Macromolecules | 1988

Equilibrium binding of mixed micelles to oppositely charged polyelectrolytes

Paul L. Dubin; Daniel R. Rigsbee; Leong-Ming Gan; M. A. Fallon


Journal of Pharmaceutical Sciences | 2007

Solid state chemistry of proteins: I. glass transition behavior in freeze dried disaccharide formulations of human growth hormone (hGH)

Michael J. Pikal; Daniel R. Rigsbee; Michael L. Roy


Thermochimica Acta | 2006

Using modulated DSC to investigate the origin of multiple thermal transitions in frozen 10% sucrose solutions

Liuquan (Lucy) Chang; Nathaniel Milton; Daniel R. Rigsbee; Dinesh Mishra; Xiaolin (Charlie) Tang; Leonard C. Thomas; Michael J. Pikal

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Paul L. Dubin

University of Massachusetts Amherst

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Bingquan Wang

University of Connecticut

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Carol Stotz

University of Connecticut

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Jinsong Liu

University of Connecticut

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