Daniela Oliveira

Morphometric study of Rynchotus rufescens testis throughout the year

The research aimed to study the morphologic variation of the testis, seeking to promote the selection and genetic control of those that present appreciable spermatic production throughout the year. Testis morphology of the Rynchotus rufescens partridge was investigated, analyzing the testis weight, the seminiferous tubules diameter, the thickness of the seminiferous epithelium, the amount of meiotic figures and the thickness of the tunica albuginea. Sixty male partridges were used, divided in 12 groups, and one group per month had the testis collected for the histological routine and the sections were stained using the Hematoxilin-Eosin technique. For the histological sections analysis, morphometric measures were taken, with the aid of an Image Analyzer and the resulting data were submitted to analysis of variance and to Tukeys test. Based on the histological modifications of the seminiferous epithelium and the morphometric analysis, the partridge testis morphology could be divided in four successive phases throughout the year. The reproductive phase occurred in the spring, characterized by the complete spermatogenesis process. The regression phase occurred in the summer, with the involution of the seminiferous epithelium. The rest phase took place in the fall, with spermatogonias presence and some spermatocytes beginning the meiosis. The phase of recrudescence occurred in the winter, with the recovery of the seminiferous epithelium and absence of spermatozoa. In conclusion, the characteristics analyzed revealed a variation over the year, with greater production of spermatozoa in the spring and less in the winter.

Implante de biomateriais e a consolidação óssea em cadelas submetidas à ovariossalpingo-histerectomia

The hydroxyapatite with alendronate and hydroxyapatite with collagen were evaluated in the acceleration of the bony consolidation of adult spayed bitch radius. For that, 14 adult bitches were distributed in two groups (control and spayed). Four months after ovariohysterectomy, the groups were submitted to the surgery for production of a 4mm diameter bony flaw in the distal and proximal third regions of the radius. In the distal region of the right thoracic limb, hydroxyapatite with alendronate was used. In the distal region of the left thoracic limb, hydroxyapatite with collagen was used. Any biomaterial was used in proximal part of the limb. There was a retard in bony flaws consolidation in the spayed bitches. Hydroxyapatite with alendronate showed better result, since the place it was implanted considerably increased the bony formation. Both biomaterials presented biocompatibility, verified by the absence of inflammatory reaction or other undesirable reaction.

A elasticidade do ligamento colateral medial da articulação do cotovelo de cão não advém de elastina

The literature reports that ligaments consist of connective tissue, composed by water, Type I and III collagen, several proteoglycans, some elastin and other substances. Ligaments tested in vitro with longitudinal and unidirectional tension exhibit non-linear mechanical behavior; the collagen fibers are stretched little by little, losing their undulating pattern, until reaching the maximum limit of traction and failure begins. The aim of the present study was to assess the presence of elastin in the medial collateral ligament of the elbow in adult dogs to determine whether the stretching of this ligament is due to the presence of elastic fibers, the elastic property of the collagen or the combination of both. Four joints were used from males and females in equal proportion, taking the medial collateral ligaments for the histological examination. To detect the presence of elastic fibers, sections were stained using the Weigert method. However, light microscopy revealed no elastic fibers. It was concluded that the elasticity of the canine elbow medial collateral ligament is mainly due to the undulated pattern of the collagen fibers, considering the trace amount or inexistence of elastic fibers in this structure.

Role of the oblique ligament in the integrity of the medial collateral ligament of the canine elbow joint

It was studied the arrangement of the collagen fibrils of the medial collateral ligament of the canine elbow joint and evaluated its diameter, when it was isolated or associated to the oblique ligament and loaded in tension until failure. Eighteen joints were divided in three groups. The first group had the medial collateral ligament collected and not loaded, the second group had the medial collateral ligament tested separately and the third group had both ligaments associately tested. Medial collateral ligament not submitted to strain presented a wavy and reticular pattern of the collagen fibers, which was not totally destroyed when it was loaded associated to the oblique ligament, and totally loses the reticular pattern when stretched separately. When the medial collateral ligament was loaded in tension separately, the mean collagen fibrils diameter increased in relation to the group not submitted to the tensile strain. Associated to the oblique ligament, the mean collagen fibrils diameter was the largest in the insertion area and the smallest in the mid-substance, in relation to the other groups. It was concluded that the oblique ligament could favor the integrity of the medial collateral ligament insertion area, facilitating its reconstruction after lesion with larger efficiency.

Avaliação dos perfis minerais séricos, urinários e sedimentares de ovinos recebendo dieta calculogênica

A urolitiase obstrutiva em pequenos ruminantes e uma doenca metabolica de etiologia multifatorial com distribuicao mundial. A elevacao da concentracao urinaria de solutos, minerais ionizados (cristaloides) que formam cristais insoluveis e citada por alguns autores como o fator mais importante. Assim, o conhecimento do perfil mineral dos animais submetidos a dietas calculogenicas e a composicao quimica dos urolitos tornam-se ferramentas eficazes na prevencao da doenca. Neste estudo, foram utilizados 14 ovinos higidos, machos (nao castrados), da raca Santa Ines, com idade aproximada de 90 dias, distribuidos em dois grupos (G1 - sem vitamina C e G2 - com vitamina C) e alimentados com dieta calculogenica. A analise dos perfis minerais, sericos e urinarios revelou completo desbalanceamento na relacao entre concentracoes de calcio, fosforo e magnesio, havendo elevacao expressiva do fosforo e do magnesio e diminuicao substancial do calcio. Com isso, a analise bioquimica dos urolitos demonstrou que o calcio esteve presente em 50% das amostras analisadas.