Danielle Gomes

Evaluation of the Pathogenicity of the Bacteroides fragilis Toxin Gene Subtypes in Gnotobiotic Mice

Enterotoxigenic Bacteroides fragilis (ETBF) strains produce a metalloprotease toxin (BFT) related to diarrheal disease in animals, young children, and adults. Three different isoforms of the enterotoxin, designated BFT-1, BFT-2, and BFT-3, have been identified and sequenced. In the present study, the pathogenicity of the ETBF strains carrying bft-1 or bft-2 was evaluated. Each toxin gene subtype of ETBF (bft-1 or bft-2) was intragastrically monoassociated to germ-free mice during 10 days and histopathological data from intestines and liver compared with those from mice monoassociated to a non-enterotoxigenic B. fragilis. Histopathological alterations were observed in all groups of animals related to ETBF. These alterations were characterized mainly by ulceration, edema, and inflammatory infiltration in intestine. However, these lesions were slightly more severe in mice monoassociated with bft-2 subtype. No alteration or lesion was observed in animals associated with the non-enterotoxigenic B. fragilis. In conclusion, strains harboring bft-1 or bft-2 gene subtypes were able to induce histopathological alterations in intestine of a gnotobiotic mice model and it could explain the effect produced for the enterotoxin.

Relación entre capacidad funcional y función diastólica en el infarto reciente

ABSTRACT | The myocardial infarction (MI) alters left ven -tricle diastolic function (LVDF) in different grades, which may reflect on functional capacity (FC). This study aimed to assess, in patients with recent MI, the relation between LVDF and FC evaluated by the distance covered during the six minute walking test (6MWT). Fifty-six uncomplicat-ed MI inpatients were selected after discharge from the coronary care unit and submitted to tests. Statistic analy-ses were carried out considering all patients for correla-tions and groups according to the classification of LVDF to comparison. It was found correlation between lateral wave a’ (later diastole) and 6MWD (r=-0.320; p=0.023) and no difference between FC and LVDF between groups. Blood pressure and heart rate had physiologic responses. The correlation indicates that the impairment of early diastole expands the role of atrial contraction in CF, reinforcing the need for evaluation of these patients still in the hospital. The physiological responses related to the six minute walk -ing test reinforce the feasibility of its use after recent MI.Keywords | exercise test; echocardiography; myocardial infarction; diastole. RESUMO | O infarto do miocardio (IM) altera a funcao dias-tolica (FD) do ventriculo esquerdo (VE) em diferentes graus, o que pode refletir na capacidade funcional (CF). O objetivo deste estudo foi avaliar, apos IM recente, a relacao entre a FD do VE por meio de ecocardiografia Doppler e a CF estimada por meio da distância percorrida no teste de caminhada de

Relação entre capacidade funcional e função diastólica no infarto recente

ABSTRACT | The myocardial infarction (MI) alters left ven -tricle diastolic function (LVDF) in different grades, which may reflect on functional capacity (FC). This study aimed to assess, in patients with recent MI, the relation between LVDF and FC evaluated by the distance covered during the six minute walking test (6MWT). Fifty-six uncomplicat-ed MI inpatients were selected after discharge from the coronary care unit and submitted to tests. Statistic analy-ses were carried out considering all patients for correla-tions and groups according to the classification of LVDF to comparison. It was found correlation between lateral wave a’ (later diastole) and 6MWD (r=-0.320; p=0.023) and no difference between FC and LVDF between groups. Blood pressure and heart rate had physiologic responses. The correlation indicates that the impairment of early diastole expands the role of atrial contraction in CF, reinforcing the need for evaluation of these patients still in the hospital. The physiological responses related to the six minute walk -ing test reinforce the feasibility of its use after recent MI.Keywords | exercise test; echocardiography; myocardial infarction; diastole. RESUMO | O infarto do miocardio (IM) altera a funcao dias-tolica (FD) do ventriculo esquerdo (VE) em diferentes graus, o que pode refletir na capacidade funcional (CF). O objetivo deste estudo foi avaliar, apos IM recente, a relacao entre a FD do VE por meio de ecocardiografia Doppler e a CF estimada por meio da distância percorrida no teste de caminhada de

Relationship between functional capacity and diastolic function in early myocardial infarction

ABSTRACT | The myocardial infarction (MI) alters left ven -tricle diastolic function (LVDF) in different grades, which may reflect on functional capacity (FC). This study aimed to assess, in patients with recent MI, the relation between LVDF and FC evaluated by the distance covered during the six minute walking test (6MWT). Fifty-six uncomplicat-ed MI inpatients were selected after discharge from the coronary care unit and submitted to tests. Statistic analy-ses were carried out considering all patients for correla-tions and groups according to the classification of LVDF to comparison. It was found correlation between lateral wave a’ (later diastole) and 6MWD (r=-0.320; p=0.023) and no difference between FC and LVDF between groups. Blood pressure and heart rate had physiologic responses. The correlation indicates that the impairment of early diastole expands the role of atrial contraction in CF, reinforcing the need for evaluation of these patients still in the hospital. The physiological responses related to the six minute walk -ing test reinforce the feasibility of its use after recent MI.Keywords | exercise test; echocardiography; myocardial infarction; diastole. RESUMO | O infarto do miocardio (IM) altera a funcao dias-tolica (FD) do ventriculo esquerdo (VE) em diferentes graus, o que pode refletir na capacidade funcional (CF). O objetivo deste estudo foi avaliar, apos IM recente, a relacao entre a FD do VE por meio de ecocardiografia Doppler e a CF estimada por meio da distância percorrida no teste de caminhada de