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Dive into the research topics where Daniëlle Peterse is active.

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Featured researches published by Daniëlle Peterse.


Reproductive Sciences | 2015

Dysregulation of Lysyl Oxidase Expression in Lesions and Endometrium of Women With Endometriosis

Lynnette A. Ruiz; Perla M. Báez-Vega; Abigail Ruiz; Daniëlle Peterse; Janice B. Monteiro; Nabal Bracero; Pedro Beauchamp; Asgerally T. Fazleabas; Idhaliz Flores

Lysyl oxidases (LOXs) are enzymes involved in collagen deposition, extracellular membrane remodeling, and invasive/metastatic potential. Previous studies reveal an association of LOXs and endometriosis. We aimed to identify the mechanisms activated by upregulation of lysyl oxidases (LOX) in endometriotic cells and tissues. We hypothesized that LOX plays a role in endometriosis by promoting invasiveness and epithelial to mesenchymal transition (EMT). Methods: The LOX protein expression levels were measured by immunohistochemistry in lesions and endometrium on a tissue microarray (TMA) and in endometrial biopsies from patients and controls during the window of implantation (WOI). Estradiol regulation of LOX expression was determined by quantitative polymerase chain reaction (qPCR). Proliferation, invasion, and migration assays were performed in epithelial (endometrial epithelial cell), endometrial (human endometrial stromal cell), and endometriotic cell lines (ECL and 12Z). Pathway-focused multiplex qPCR was used to determine transcriptome changes due to LOX overexpression. Results: LOX protein was differentially expressed in ovarian versus peritoneal lesions. During WOI, LOX levels were higher in luminal epithelium of patients with endometriosis-associated infertility compared to controls. Invasive epithelial cell lines expressed higher levels of LOX than noninvasive ones. Transfection of LOX into noninvasive epithelial cells increased their migration in an LOX inhibitor-sensitive manner. Overexpression of LOX did not fully induce EMT but the expression of genes related to fibrosis and extracellular matrix remodeling were dysregulated. Conclusions: This study documents that expression of LOX is differentially regulated in endometriotic lesions and endometrium. A role for LOX in mediating proliferation, migration, and invasion of endometrial and endometriotic cells was observed, which may be implicated in the establishment and progression of endometriotic lesions.


Reproductive Sciences | 2017

The Presence of Endometrial Cells in Peritoneal Fluid of Women With and Without Endometriosis

Dorien F. O; Tania Roskams; Kathleen Van den Eynde; Arne Vanhie; Daniëlle Peterse; Christel Meuleman; Carla Tomassetti; Karen Peeraer; Thomas D’Hooghe; Amelie Fassbender

To reinforce Sampson’s theory of retrograde menstruation in the pathogenesis of endometriosis, proof should be provided that during menstruation endometrial cells are present in peritoneal fluid (PF). We hypothesize that the prevalence of PF samples containing endometrial cells is higher in patients with endometriosis than in controls without endometriosis during menstruation. We selected from our biobank PF samples of 17 reproductive-age women with (n = 9) or without (n = 8) endometriosis who had received a diagnostic laparoscopy for investigation of pain/infertility. Peritoneal fluid had been collected during laparoscopy in the menstrual phase of the cycle, centrifuged, and the resulting pellet was stored at −80°C. About 5-μm sections of frozen PF pellets were stained using the Dako Envision Flex system with primary antibodies against epithelial cell adhesion molecule (Ep-CAM; endometrial epithelial cells), CD10 (endometrial stromal cells), prekeratin (epithelial/mesothelial cells), vimentin (endometrial/mesothelial/immune cells), calretinin (mesothelial cells), and CD68 (macrophages). The PF cells positive for Ep-CAM were detected in 5 of 9 patients with endometriosis and 6 of 8 controls (P = .62). CD10 stained positively in 6 of the 9 patients with endometriosis and 3 of the 8 controls (P = .35). Calretinin and prekeratin staining showed the presence of mesothelial cells in all pellets. Vimentin stained approximately 100% of the PF cells. CD68+ macrophages represented >50% of cells in all pellets. The prevalence of PF samples containing endometrial epithelial and stromal cells was not higher in patients with endometriosis than in controls without endometriosis during menstruation. Our findings question the relevance of endometrial cells in PF for the pathogenesis of endometriosis and support the importance of other mechanisms such as immune dysfunction and/or endometrial stem cells.To reinforce Sampsons theory of retrograde menstruation in the pathogenesis of endometriosis, proof should be provided that during menstruation endometrial cells are present in peritoneal fluid (PF). We hypothesize that the prevalence of PF samples containing endometrial cells is higher in patients with endometriosis than in controls without endometriosis during menstruation. We selected from our biobank PF samples of 17 reproductive-age women with (n = 9) or without (n = 8) endometriosis who had received a diagnostic laparoscopy for investigation of pain/infertility. Peritoneal fluid had been collected during laparoscopy in the menstrual phase of the cycle, centrifuged, and the resulting pellet was stored at -80°C. About 5-(j,m sections of frozen PF pellets were stained using the Dako Envision Flex system with primary antibodies against epithelial cell adhesion molecule (Ep-CAM; endometrial epithelial cells), CD 10 (endometrial stromal cells), prekeratin (epithelial/mesothelial cells), vimentin (endometrial/mesothelial/immune cells), calretinin (mesothelial cells), and CD68 (macrophages). The PF cells positive for Ep-CAM were detected in 5 of 9 patients with endometriosis and 6 of 8 controls (P =.62). CD10 stained positively in 6 of the 9 patients with endometriosis and 3 of the 8 controls (P =.35). Calretinin and prekeratin staining showed the presence of mesothelial cells in all pellets. Vimentin stained approximately 100% of the PF cells. CD68+ macrophages represented >50% of cells in all pellets. The prevalence of PF samples containing endometrial epithelial and stromal cells was not higher in patients with endometriosis than in controls without endometriosis during menstruation. Our findings question the relevance of endometrial cells in PF for the pathogenesis of endometriosis and support the importance of other mechanisms such as immune dysfunction and/or endometrial stem cells.


Reproductive Sciences | 2016

Laparoscopic Surgery: A New Technique to Induce Endometriosis in a Mouse Model.

Daniëlle Peterse; Amelie Fassbender; Dorien F. O; Arne Vanhie; Philippa T. K. Saunders; Joris Vriens; M. Mercedes Binda; Thomas D’Hooghe

Background: This prospective pilot study was designed to induce endometriosis in a mouse model using laparoscopy, a less invasive and more precise approach than laparotomy. We aimed to achieve a peritoneal implant rate of at least 50% by varying both duration of anesthesia and intra-abdominal insufflation pressure. Methods: Female BALB/cANnCrl mice in metestrus or diestrus were used as donors (n = 5) or recipients (n = 20) of uterine transplant tissue. Each recipient mouse was laparoscopically inoculated with 10 uterine pieces (range: 10-12) from donor mice into the abdominal cavity. Before starting the study, recipient mice were randomly assigned to 4 groups with variable duration of anesthesia (ketamine/xylazine or pentobarbital) and variable intra-abdominal pressure (5 or 15 mm Hg). One week after laparoscopy, endometriosis incidence and peritoneal implant take rate were documented visually during laparotomy. The retrieved lesions were histologically analyzed. Results: Laparoscopic inoculation of uterine pieces in recipient mice resulted in an endometriosis incidence of 100% (20/20 animals) and an individual peritoneal implant take rate of 60% (121/206), ranging from 17% (2/12) till 83% (10/12), without differences between the 4 subgroups, and with a histological confirmation rate of 92% (58/63). Conclusions: To the best our knowledge, this is the first report showing that endometriosis can be induced by laparoscopic surgery in rodents, with a 100% incidence and a median peritoneal implant take rate of 60%. This laparoscopic model offers important advantages over traditional laparotomy models that are limited by surgery-associated trauma and/or adhesion formation.


Journal of Minimally Invasive Gynecology | 2017

Of Mice and Women: A Laparoscopic Mouse Model for Endometriosis

Daniëlle Peterse; M. Mercedes Binda; Dorien F. O; Arne Vanhie; Amelie Fassbender; Joris Vriens; Thomas D'Hooghe

STUDY OBJECTIVE To demonstrate how a novel laparoscopic approach allows the development of a mouse model for endometriosis after seeding menstrual endometrium from donor mice into the abdominal cavity of syngeneic recipient mice. DESIGN A step-by-step video description of the techniques used to adapt the estrous cycle of mice towards a menstrual cycle and to subsequently induce endometriosis via laparoscopic seeding of menstrual endometrium. SETTING University research institute. ETHICS All animal experiments were ethically approved by KU Leuven, Belgium (ethical approval number: P031/2013). INTERVENTIONS, MEASUREMENTS, AND MAIN RESULTS Oophorectomized female C57BL/6JRj mice received a series of estrogen injections. Next, a progesterone pellet was administered, together with a second series of estrogen injections. In addition, decidualization of the endometrium was induced with an intrauterine sesame oil stimulus. Four days later the progesterone pellet was removed and menstruation started [1]. Five hours after the progesterone pellet was removal the uterus was harvested, and the menstrual endometrium was dissected and seeded into the abdominal cavity of syngeneic recipient mice to induce endometriosis [2] using a laparoscopic approach [3]. Uterus and lesions were removed from the recipient mice 1 week after induction, and tissues were immunohistochemically stained for H&E, vimentin, and cytokeratin. CONCLUSION In this video we show a novel methodology to induce endometriosis in mice using laparoscopic inoculation of syngeneic menstrual endometrium, mimicking Sampsons theory of retrograde menstruation [4]. Compared with currently available rodent models, our model offers a less invasive and more physiologic way for fundamental and preclinical endometriosis research, with a high endometriosis incidence and lesion take rate.


Gynecologic and Obstetric Investigation | 2017

Evaluation of Total, Active, and Specific Myeloperoxidase Levels in Women with and without Endometriosis

Dorien F. O; Etienne Waelkens; Daniëlle Peterse; Dan I. Lebovic; Christel Meuleman; Carla Tomassetti; Karen Peeraer; Alexandra Alvarez Real; Alain Bosseloir; Thomas D'Hooghe; Amelie Fassbender

Myeloperoxidase (MPO) is a proinflammatory enzyme and a marker for neutrophil activation and oxidative stress. Since oxidative stress and inflammation are linked to the pathogenesis of endometriosis, we hypothesized that the total, active, and specific (active/total) MPO levels were significantly different in plasma of women with and without endometriosis. Samples were selected from our biobank from women with endometriosis (n = 212) and controls without endometriosis (n = 121) across the menstrual cycle. Total MPO plasma levels were measured by immunoassay and MPO activity by enzymatic assay. Total and active MPO levels did not differ significantly among endometriosis cases and controls, whereas the specific MPO activity was significantly lower in women with endometriosis than that in controls (p = 0.0159). After the subdivision of control patients into women with a normal pelvis and women with other benign gynecological disorders, a significant difference was observed only between women with endometriosis and women with other benign gynecological disorders (p = 0.0266). In conclusion, systemic MPO levels may not be suited as a single biomarker for endometriosis. Our data support the involvement of MPO in other gynecological disorders but do not provide any evidence for an association with endometriosis.


Reproductive Sciences | 2018

Optimization of Endometrial Decidualization in the Menstruating Mouse Model for Preclinical Endometriosis Research

Daniëlle Peterse; Katrien De Clercq; Chloë Goossens; M. Mercedes Binda; Dorien O; Philippa T. K. Saunders; Joris Vriens; Amelie Fassbender; Thomas M D'Hooghe

Background: To induce endometrial decidualization in rodents, an intrauterine oil stimulus can be delivered via the nontraumatic vagina or via the traumatic laparotomy. However, there is considerable variation in amount of decidualization using these inducing methods. Therefore, we studied which oil delivery route could achieve the highest rate of endometrial decidualization along the full length of both uterine horns. Methods: To induce decidualization, ovariectomized C57Bl/6J mice were injected with estrogen (100 ng/day; 3 days). A progesterone pellet (5 mg) was implanted subcutaneously, followed by estrogen injections (5 ng/day; 3 days). Oil (20 µL/horn) was injected in the uterus via laparotomy, laparoscopy, or vagina. Four days later, the pellet was removed, followed by hysterectomy after 4 to 6 hours. Endometrial decidualization was evaluated macroscopically and microscopically using hematoxylin and eosin and desmin staining. Furthermore, uterine weight and hormone levels were measured. Results: The proportion of animals with macroscopic bicornuate decidualization was higher after laparoscopic (83%) and laparotomic (89%) injection than after sham injection (11%). Furthermore, macroscopic bicornuate decidualization was significantly higher after laparotomic injection (89%) compared to the vaginal injection (38%). Uterine weight and endometrial surface area were significantly higher in both laparotomy and laparoscopy groups compared to the sham group, while the relative desmin-positive endometrial surface area was only significantly different between the laparotomy and the sham animals. Conclusion: Methods using laparoscopic and laparotomic intrauterine oil injection resulted in a higher amount of decidualized endometrium compared to sham injection, although further optimization is needed to reach full bicornuate decidualization.


International Journal of Molecular Sciences | 2018

Functional Expression of TRP Ion Channels in Endometrial Stromal Cells of Endometriosis Patients

Eleonora Persoons; Aurélie Hennes; Katrien De Clercq; Rita Van Bree; Goede Vriens; Dorien O; Daniëlle Peterse; Arne Vanhie; Christel Meuleman; Thomas Voets; Carla Tomassetti; Joris Vriens

Endometriosis is a common gynecological disease that is characterized by the presence of functional endometrial-like lesions in the abdominal cavity. Aside from epithelial cells, these lesions consist of stromal cells that have the capacity to migrate, adhere, proliferate, and induce neuro- and lymphangiogenesis, which allows them to survive at ectopic locations. However, the exact underlying mechanisms that regulate these changes are yet to be elucidated. The common ground of these processes, however, is the second messenger, calcium. In this regard, members of the superfamily of transient receptor potential (TRP) ion channels, which are known to be calcium-permeable and expressed in the endometrium, have emerged as key regulators. Here, we assessed the molecular and functional expression of TRP channels in stromal cells isolated from the eutopic endometrium of endometriosis patients and controls. Using RT-qPCR, high mRNA levels of TRPV2, TRPV4, TRPM4, TRPM7, TRPC1, TRPC3, TRPC4, and TRPC6 were observed in the whole endometrium throughout the menstrual cycle. Additionally, and in line with previous reports of control patients, TRPV2, TRPV4, TRPC1/4, and TRPC6 were present in human endometrial stromal cells (hESC) from endometriosis patients both at the molecular and functional level. Moreover, proliferation and migration assays illustrated that these parameters were not affected in stromal cells from endometriosis patients. Furthermore, comparison between eutopic and ectopic endometrial samples revealed that the RNA expression pattern of TRP channels did not differ significantly. Collectively, although a functional expression of specific ion channels in hESCs was found, their expression did not correlate with endometriosis.


Twin Research and Human Genetics | 2015

Independent Replication and Meta-Analysis for Endometriosis Risk Loci.

Yadav Sapkota; Amelie Fassbender; Lisa Bowdler; Jenny N. Fung; Daniëlle Peterse; Dorien O; Grant W. Montgomery; Dale R. Nyholt; Thomas D'Hooghe


School of Biomedical Sciences; Faculty of Health; Institute of Health and Biomedical Innovation | 2017

Meta-analysis identifies five novel loci associated with endometriosis highlighting key genes involved in hormone metabolism

Yadav Sapkota; Valgerdur Steinthorsdottir; Andrew P. Morris; Amelie Fassbender; Nilufer Rahmioglu; Immaculata De Vivo; Julie E. Buring; Futao Zhang; Todd L. Edwards; Sarah Jones; Dorien O; Daniëlle Peterse; Kathryn M. Rexrode; Paul M. Ridker; Andrew J. Schork; Stuart MacGregor; Nicholas G. Martin; Christian M. Becker; Sosuke Adachi; Kosuke Yoshihara; Takayuki Enomoto; Atsushi Takahashi; Yoichiro Kamatani; Koichi Matsuda; Michiaki Kubo; Gudmar Thorleifsson; Reynir Tómas Geirsson; Unnur Thorsteinsdottir; Leanne Wallace; Thomas Werge


School of Biomedical Sciences; Faculty of Health; Institute of Health and Biomedical Innovation | 2017

Analysis of potential protein-modifying variants in 9000 endometriosis patients and 150000 controls of European ancestry

Yadav Sapkota; Immaculata De Vivo; Valgerdur Steinthorsdottir; Amelie Fassbender; Lisa Bowdler; Julie E. Buring; Todd L. Edwards; Sarah Jones; Dorien O; Daniëlle Peterse; Kathryn M. Rexrode; Paul M. Ridker; Andrew J. Schork; Gudmar Thorleifsson; Leanne Wallace; Peter Kraft; Andrew P. Morris; Dale R. Nyholt; Digna R. Velez Edwards; Mette Nyegaard; Thomas D’Hooghe; Daniel I. Chasman; Kari Stefansson; Stacey A. Missmer; Grant W. Montgomery

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Amelie Fassbender

Katholieke Universiteit Leuven

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Arne Vanhie

Katholieke Universiteit Leuven

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Dorien O

Katholieke Universiteit Leuven

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Christel Meuleman

Katholieke Universiteit Leuven

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Thomas D'Hooghe

Katholieke Universiteit Leuven

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Thomas D’Hooghe

Katholieke Universiteit Leuven

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Dorien F. O

Katholieke Universiteit Leuven

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Joris Vriens

Katholieke Universiteit Leuven

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Yadav Sapkota

QIMR Berghofer Medical Research Institute

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Carla Tomassetti

Katholieke Universiteit Leuven

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