Danladi Amodu Ameh

In vitro trypanocidal effect of methanolic extract of some Nigerian savannah plants

Methanol extracts from twenty three plants harvested from the Savannah vegetation belt of Nigeria were analyzed in vitro for trypanocidal activity against Trypanosoma brucei brucei and Trypanosoma congolense at concentrations of 4 mg/ml, 0.4 mg/ml and 0.04 mg/ml. Extracts of Khaya senegalensis, Piliostigma reticulatum, Securidaca longepedunculata and Terminalia avicennoides were strongly trypanocidal to both organisms while extracts of Anchomanes difformis, Cassytha spp, Lannea kerstingii, Parkia clappertioniana, Striga spp, Adansonia digitata and Prosopis africana were trypanocidal to either T. brucei brucei or T. congolense. These findings provide evidence of the effects of some plants in the traditional management of trypanosomiasis.

Antihypertensive effect of roselle (Hibiscus sabdariffa) calyx infusion in spontaneously hypertensive rats and a comparison of its toxicity with that in Wistar rats

The LD50 of roselle calyx extract and its effect on blood pressure were determined. The LD50 was found to be above 5000 mg kg−1. Roselle calyx infusion was found to lower significantly (p<0·05) both systolic and diastolic pressure in spontaneously hypertensive and normotensive Wistar–Kyoto rats at tested doses of 500 and 1000 mg kg−1 body weight. The reduction in blood pressure in both groups was positively correlated with weight. Continuous consumption of the infusion at 1000 mg kg−1 was discovered to lead to sudden death in spontaneously hypertensive rats but not in Wistar‐Kyoto rats. Water intake was not significantly different (p>0·05) in the control groups of the two strains of rats used, neither was there a significant difference in their urine output. The water intake in the treated spontaneously hypertensive and normotensive rats was not different from the corresponding control groups. However the urine output of the treated spontaneously hypertensive rats was significantly higher. A significant decrease in serum creatinine, cholesterol, and glucose in the treated rats compared with the control as well as a significant increase in serum uric acid was observed. The serum proteins (albumin and total protein) in the treated rats when compared with the control groups was not changed significantly. Copyright

Quantitative changes in gastric mucosal glycoproteins: effect of cholinergic agonist and vagal nerve stimulation in the rat

The role of the vagus nerve and cholinergic mechanisms in the control of the rat gastric mucin and protein (PROT) release in vivo was investigated. Under urethane anaesthesia (1.25 g kg−1), the rats had their gastric lumen perfused with saline. Mucus secretion was measured as a function of adherent mucus on the mucosa surface and the luminal content of sialic acids (SIA), galactose (GAL), pyruvate and PROT. Electrical stimulation of the vagi significantly increased the levels of mucus (3.23 ± 025 μg g−1 tissue, P < 0.05), free sialic acid (FS) (0.18 ± 0.04 mg mL−1, P < 0.05) and PROT (0.25 ± 0.003 mg mL−1, P < 0.05) when compared with control animals. Bilateral cervical vagotomy had no significant effect on adherent mucus or basal levels of PROT, SIA and GAL (P > 0.05) with respect to the control. In both vagotomized and vagal intact animals, the cholinergic agonist (carbachol, 200 mg kg−1) significantly increased PROT, adherent mucus and FS (P < 0.05) and decreased bound sialic acid (P > 0.05). There were no visible haemorrhagic streaks on the gastric mucosa of vagotomized, vagal intact and carbachol‐treated animals. The results suggest that vagus nerve does not exert a tonic control on gastric glycoprotein secretion in vivo and that cholinergic effect on the mucus secreting cells may be implemented via the intrinsic nerves of the enteric nervous system.

A Preliminary Study of the Mechanism of Hypoglycaemic Activity of Nauclea latifolia Leaf Ethanolic Extract

Abstract The aim of this study was to determine the possible underlying mechanism of the hypoglycaemic activity of the ethanolic extract of Nauclea latifolia leaves in rats. The extract, glibenclamide or water was administered orally in a glucose, sucrose and maltose tolerance tests. In addition, the effect of the extract on α-glucosidase enzymes was also studied. The ethanolic extract at 200mg/kg body weight inhibited the increase in glucose level after both oral and intraperitoneal glucose loads as did glibenclamide. The extract also dose dependently inhibited both maltase and sucrase activities in vitro but not in vivo. The hypoglycaemic effect of N. latifolia leaf ethanolic extract thus appears to be most probably exerted through a mechanism similar to that of glibenclamide which is related to increased insulin release from pancreatic β-cells.

An analysis of dietary fiber and fecal fiber components including pH in rural Africans with colorectal cancer

Background/Aims Colorectal cancer (CRC) is now a major public health problem with heavy morbidity and mortality in rural Africans despite the lingering dietary fiber-rich foodstuffs consumption. Studies have shown that increased intake of dietary fiber which contribute to low fecal pH and also influences the activity of intestinal microbiota, is associated with a lowered risk for CRC. However, whether or not the apparent high dietary fiber consumption by Africans do not longer protects against CRC risk is unknown. This study evaluated dietary fiber intake, fecal fiber components and pH levels in CRC patients. Methods Thirty-five subjects (CRC=21, control=14), mean age 45 years were recruited for the study. A truncated food frequency questionnaire and modified Goering and Van Soest procedures were used. Results We found that all subjects consumed variety of dietary fiber-rich foodstuffs. There is slight preponderance in consumption of dietary fiber by the control group than the CRC patients. We also found a significant difference in the mean fecal neutral detergent fiber, acid detergent fiber, hemicellulose, cellulose and lignin contents from the CRC patients compared to the controls (P<0.05). The CRC patients had significantly more fecal pH level than the matched apparently healthy controls (P=0.017). Conclusions The identified differences in the fecal fiber components and stool pH levels between the 2 groups may relate to CRC incidence and mortality in rural Africans. There is crucial need for more hypothesis-driven research with adequate funding on the cumulative preventive role of dietary fiber-rich foodstuffs against colorectal cancer in rural Africans “today.”

Abstract A19: Dietary-fiber and fecal fiber contents including pH in rural Africans with colorectal cancer

Studies have shown improved/increased intake of dietary fiber and low fecal pH are associated with a lowered risk for colorectal cancer. This study aimed to compare dietary fiber intake, fecal neutral detergent fiber (NDF) content, acid detergent fiber (ADF), cellulose, hemicellulose, lignin and fecal pH level of colorectal cancer patients and matched apparently healthy control group in rural Africans in Zaria, Nigeria. Twenty-one subjects of mean age 44.7 years who are diagnosed with colorectal cancer by histology and 14 apparently healthy matched control groups with mean age 45.2 years were recruited for this study. A predefined semi structured food frequency questionnaire (FFQ) was used to access the dietary fiber intake by the subjects. The fecal NDF content was analyzed by extracting 1 g of fecal sample with a mixture of ethanol/ether and refluxed with decalin and sodium sulphite. The extract was suctioned and washed with acetone to yield the total NDF. The NDF was refluxed with an acid detergent solution and dried overnight to yield the ADF. The differences in the NDF and ADF gave the total hemicelluloses. The differences in the weight of the ADF post-treatment with permanganate solution yielded the total lignin. The differences in weight of the permanganate residue post-treatment with 72% Sulphuric acid gave the cellulose content. A pH meter calibrated with buffers of pH 4 and 7 was used to measure the fecal pH of all the subjects. Results from the FFQ show all subjects consumed variety of dietary fiber food in significant amount. However, there was a significant difference in the fecal NDF, ADF, hemicellulose, lignin and cellulose contents from the colorectal cancer patients when compared with the control group (p Citation Format: Mohammed Faruk, Sani Ibrahim, Ahmed Adamu, Abdulmumini Hassan Rafindadi, Yahaya Ukwenya, Yawale Iliyasu, Abdullahi Adamu, Mohammed Sani Shehu, Danladi Amodu Ameh, Sirajo Mohammed Aminu, Saad Aliyu Ahmed, John Idoko, Atara Ntekim, Aishatu Maude Suleiman, Khalid Zahir Shah, Kasimu Umar Adoke. Dietary-fiber and fecal fiber contents including pH in rural Africans with colorectal cancer [abstract]. In: Proceedings of the AACR International Conference: New Frontiers in Cancer Research; 2017 Jan 18-22; Cape Town, South Africa. Philadelphia (PA): AACR; Cancer Res 2017;77(22 Suppl):Abstract nr A19.

Abstract B71: First ten malignancies by ranking from a population of rural Africans in Zaria, Nigeria: A fourteen years review and report

Every environment is noted for its variability in incidences of various malignancies and subtypes. The objective of this study is to analyze the trend of malignant tumors in a population of rural Africans attending Ahmadu Bello University Teaching Hospital Zaria, Nigeria with particular emphasis on the first ten most frequently diagnosed cancers over a period of 14 consecutive years (2001-2014). Histologically diagnosed cancers in the various cancer registries of the ABUTH Zaria were included in this study. The ten most commonly presenting cancers were extracted and their demographic characteristics and other relevant clinical data documented using a standardized questionnaire. The data obtained were analyzed using SPSS software version 20.0. There were 6,553 histologically diagnosed cancers over 14 years in Ahmadu Bello Unibersity Teaching Hospital Zaria with the following in descending order: Cervical cancer 1042 (15.90%), Breast cancer 788 (12.02%), Prostate cancer 496 (7.56%), Colorectal cancer 360 (5.49%), Lymphoma 306 (4.66%), Eye/orbit cancer 170 (2.59%), Bladder cancer 169 (2.45%), Nasopharyngeal cancer 139 (2.12%), Ovarian cancers 94 (1.43%), and Liver cancer 52 (0.79%). Cervical cancer tops the list as the most frequently diagnosed cancer however, this should be noted that it is a cancer exclusive to females so also breast cancers though rarely males may present with breast cancer. Prostate cancer is a male exclusive disease therefore the percentage would have been higher if the diagnosis would have excluded the females. Of the ten most frequently diagnosed cancers, liver cancer is the tenth despite the fact that complications of viral hepatitidis are known to facilitate liver carcinogenesis and the prevalence of hepatitis C and B in the Nigerian population is 5 and 20 respectively. Citation Format: Abdulmumini Hassan Rafindadi, Surajo Mohammed Aminu, Mohammed Faruk, Yawale Iliyasu, Abdullahi Mohammed, Sani Ibrahim, Ahmed Adamu, Mohammed Sani Shehu, Abdullahi Jubril Randawa, Adamu Abdullahi, Saad Aliyu Ahmed, Maitama Yusuf Hussaini, Danladi Amodu Ameh, Ukwenya Yahaya, John Idoko, Oguntayo Olanrewaju Adekunle, Almustapha Aliyu Liman, Abdulkadir Lawal Rafindadi, Aisha Maude Suleiman, Jigo Dangude Yaro, Ahmad Bello, Bello Kumo. First ten malignancies by ranking from a population of rural Africans in Zaria, Nigeria: A fourteen years review and report. [abstract]. In: Proceedings of the Ninth AACR Conference on the Science of Cancer Health Disparities in Racial/Ethnic Minorities and the Medically Underserved; 2016 Sep 25-28; Fort Lauderdale, FL. Philadelphia (PA): AACR; Cancer Epidemiol Biomarkers Prev 2017;26(2 Suppl):Abstract nr B71.

Preventive and Therapeutic Effects of Garcinia kola Biflavonoid Fractions on some Haematological Parameters of P407 Induced Hyperlipidemic Albino Rats

The preventive and therapeutic effects of Garcinia kola biflavonoid fractions on some haematological parameters in Poloxamer 407 (P407) induced hyperlipidemic rats was studied for a period of 21 days. Sixty mixed sex Wistar rats weighing 150-200 g were divided into two major groups; Preventive group comprising the following sub-groups: normal control, P407 induced hyperlipidemic control and groups treated orally with atorvastatin as standard drug, root bark, stem bark and seed biflavonoid fractions of Garcinia kola respectively for 19 days and made hyperlipidemic with a single intraperitoneal injection on day 19. Therapeutic group comprising similar subgroups were induced by an intraperitoneal injection of P407 every 48 hours and treated with atorvastatin, root bark, stem bark and seed biflavonoid fractions for 21 days. In both groups, atorvastatin, P407 and the Garcinia kola biflavonoid fractions were administered at 10 mg/kg, 500 mg/kg and 200 mg/kg body weight respectively. Blood samples were collected from the rats in all the groups at the end of the experiment for determination of some haematological parameters levels; Packed Cell Volume (PCV), Platelet, neutrophils, lymphocytes, Haemoglobin (Hb), Mean Corpuscular Haemoglobin (MCH), Mean Corpuscular Haemoglobin Concentration (MCHC), Mean Corpuscular Volume (MCV), Red Blood Cell (RBC) Count and White Blood Cell (WBC) Count were determined using the Sysmex Haematological Autoanalyzer. In both groups, atorvastatin and all biflavonoid fractions significantly (p 0.05) change on the level of neutrophils compared to hyperlipidemic control. The seed biflavonoid fraction significantly (p 0.05) change in the levels of the other determined haematological parameters in both groups. These results suggest that Garcinia kola (root bark, stem bark and seed) biflavonoid fractions could be potential drugs for the prevention and treatment of platelet-activity thrombosis as well as erythropoietic, protective and stimulating effect.