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Dive into the research topics where Danna Zhou is active.

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Featured researches published by Danna Zhou.


Gene | 2013

A one-step RT-PCR assay to detect and discriminate porcine reproductive and respiratory syndrome viruses in clinical specimens.

Keli Yang; Yanhe Li; Zhengying Duan; Rui Guo; Zewen Liu; Danna Zhou; Fangyan Yuan; Yongxiang Tian

Outbreaks of highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) have led to large economic losses and, subsequently, have drawn great attention to its diagnosis and prevention. To facilitate rapid discrimination of HP-PRRSV from classical PRRSV (C-PRRSV), we developed a one-step RT-PCR assay. Primer specificities were evaluated with RNA extracted from 8 viral strains and our results revealed that the primers had a high specificity for PRRSV. The assay sensitivity was 25 copies/μL for both HP-PRRSV and C-PRRSV. A total of 929 serum samples were identified, of which 20.45% were HP-PRRSV-positive and 1.51% were C-PRRSV-positive, which was completely consistent with that of immunochromatochemistry and sequencing method. The proposed assay can detect the virus 2 days prior the onset of symptoms and it can be performed in 2h, thereby providing a rapid method to discriminate HP-PRRSV from C-PRRSV for the identification and prevention of PRRSV infections.


Veterinary Microbiology | 2014

Deletion of the znuA virulence factor attenuates Actinobacillus pleuropneumoniae and confers protection against homologous or heterologous strain challenge.

Fangyan Yuan; Yonghong Liao; Wujin You; Zewen Liu; Yongqiang Tan; Chengkun Zheng; BinWang; Danna Zhou; Yongxiang Tian; Weicheng Bei

The znuA gene is known to be important for growth and survival in Escherichia coli, Haemophilus spp., Neisseria gonorrhoeae, and Pasteurella multocida under low Zn(2+) conditions. This gene is also present in Actinobacillus pleuropneumoniae serotype 1; therefore, the aim of this study was to investigate the existence of a similar role for the znuA gene in the growth and virulence of this organism. A precisely defined ΔznuA deletion mutant of A. pleuropneumoniae was constructed based on the sequence of the wild-type SLW01 using transconjugation and counterselection. This mutation was found to be lethal in low-Zn(2+) medium. Furthermore, the ΔznuA mutant strain exhibited attenuated virulence (≥22-fold) as well as reduced mortality and morbidity in a murine (Balb/C) model of infection. The majority of the bacteria were cleared from the lungs within 2 weeks. The ΔznuA mutant strain caused no adverse effects in pigs at doses of up to 1.0×10(9) CFU/mL. The ΔznuA mutant strain induced a significant immune response and conferred 80% and 100% protection on immunised pigs against challenge with A. pleuropneumoniae strains belonging to homologous or heterologous serovars, respectively, compared to the blank controls. The data obtained in this study indicate the potential of the mutant ΔznuA strain for development as a live vaccine capable of inducing reliable cross-serovar protection following intratracheal immunisation.


Archives of Virology | 2011

Multiple amino acid variations in the nonstructural proteins of swine Japanese encephalitis virus alter its virulence in mice

Rui Wu; Yongxiang Tian; Junhua Deng; Keli Yang; Wangwang Liang; Rui Guo; Zhengying Duan; Zewen Liu; Danna Zhou; Diping Xu

In a previous study, we performed serial brain-to-brain passages of swine Japanese encephalitis virus in mice and sequenced the complete genomes of the F5 and F20 passaged mouse-adapted variants. In the current study, we analyzed the differences between their genome sequences and found 12 amino acid substitutions in the nonstructural proteins. We also assessed the growth characteristics of these two variants in mammalian cells in vitro and in vivo. Our investigations revealed that the F20 variant had enhanced growth characteristics and modified virulence compared with the F5 variant. We therefore conclude that multiple amino acid substitutions in the nonstructural proteins of swine Japanese encephalitis virus alter its virulence in mice.


PeerJ | 2018

Epidemiological and genetic characteristics of swine pseudorabies virus in mainland China between 2012 and 2017

Ying Sun; Wan Liang; Qingyun Liu; Tingting Zhao; Hechao Zhu; Lin Hua; Zhong Peng; Xibiao Tang; Charles W. Stratton; Danna Zhou; Yongxiang Tian; Huanchun Chen; Bin Wu

The outbreak of pseudorabies (PR) in many Bartha-K61 vaccinated farms in China in late 2011 has seriously damaged the pig industry of one of the largest producers of pork products in the world. To understand the epidemiological characteristics of the pseudorabies virus (PRV) strains currently prevalent in China, a total of 16,256 samples collected from pig farms suspected of PRV infection in 27 Provinces of China between 2012 and 2017 were evaluated for detection of PRV. Since the extensive use of gE-deleted PRV vaccine in China, the PRV-gE was applied for determining wild-type virus infection by PCR. Of the 16,256 samples detected, approximately 1,345 samples were positive for the detection of PRV-gE, yielding an average positive rate of 8.27%. The positive rates of PRV detection from 2012 to 2017 were 11.92% (153/1284), 12.19% (225/1846), 6.70% (169/2523), 11.10% (269/2424), 5.57% (147/2640), and 6.90% (382/5539), respectively. To understand the genetic characteristics of the PRV strains currently circulating, 25 PRV strains isolated from those PRV-gE positive samples were selected for further investigation. Phylogenetic analysis based on gB, gC, and gE showed that PRV strains prevalent in China had a remarkably distinct evolutionary relationship with PRVs from other countries, which might explain the observation that Bartha-K61 vaccine was unable to provide full protection against emergent strains. Sequence alignments identified many amino acid changes within the gB, gC, and gE proteins of the PRVs circulating in China after the outbreak compared to those from other countries or those prevalent in China before the outbreak; those changes also might affect the protective efficacy of previously used vaccines in China, as well as being associated in part with the increased virulence of the current PRV epidemic strains in China.


Microbial Pathogenesis | 2018

Development of sandwich Enzyme-Linked Immunosorbent Assay for the detection of porcine epidemic diarrhea virus in fecal samples

Rui Gui; Hong-yan Shi; Wei Liu; Li Feng; Keli Yang; Rui Guo; Wan Liang; Fangyan Yuan; Zhengying Duan; Zewen Liu; Khalid Mehmood; Riaz Hussain; Danna Zhou; Yongxiang Tian

Porcine epidemic diarrhea virus (PEDV) causes acute diarrhea and dehydration in new-born piglets with subsequent economic losses to swine industry. In the current study, gene encoding of 381aa-792aa spike protein (S1) with the main epitope relative to virus neutralization of PEDV was amplified by RT-PCR and inserted into vector pET-30A(+). The plasmid was transferred into Escherichia coli BL21 (DE3). Meanwhile, recombinant protein expression was induced by isopropy1-β-galactopyranoside (IPTG). After denaturation and renaturation of inclusion bodies, the S1 protein was obtained by using purified recombinant S1 protein in immunized female BALB/c mice. Monoclonal antibodies (MAb) against S1 protein, named 4C7 by hybridoma technique were gained successfully. The result showed that MAb can specifically respond to S1 protein and PEDV via ELISA, Western bolt and immunofluorescence assay methods. A sandwich ELISA (S-ELISA) was established by using the captured monoclonal antibodies 4C7. The sensitivity and specificity were compared between S-ELISA and RT-PCR, which showed similar sensitivity and specificity. This work indicated that S-ELISA would be a significant tool alongside a specific diagnostic reagent for PEDV in future.


Viruses | 2017

A Multiplex RT-PCR Assay to Detect and Discriminate Porcine Reproductive and Respiratory Syndrome Viruses in Clinical Specimens

Keli Yang; Yongxiang Tian; Danna Zhou; Zhengying Duan; Rui Guo; Zewen Liu; Fangyan Yuan; Wei Liu

Outbreaks of highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) have led to large economic losses in China. The attenuated vaccine (HP-PRRSV JXA1-R) was used to control HP-PRRSV. However, in recent years, co-infection with classical PRRSV (C-PRRSV), HP-PRRSV, and/or HP-PRRSV JXA1-R has been increasing in China, resulting in a significant impact on PRRSV diagnostics and management. To facilitate rapid discrimination of HP-PRRSV JXA1-R from HP-PRRSV and C-PRRSV, a multiplex RT-PCR assay for the visual detection of HP-PRRSV JXA1-R, HP-PRRSV, and C-PRRSV was established and evaluated with reference PRRSV strains and clinical samples. Primer specificities were evaluated with RNA/DNA extracted from 10 viral strains, and our results revealed that the primers had a high specificity for PRRSV. The assay sensitivity was 24 copies/μL for PRRSVs. A total of 516 serum samples were identified, of which 12.21% (63/516) were HP-PRRSV-positive, 2.33% (12/516) were HP-PRRSV JXA1-R-positive, and 1.16% (6/516) were C-PRRSV-positive, respectively, which was completely consistent with the sequencing method. The high specificity, sensitivity, and reliability of the multiplex RT-PCR assay described in this study indicate that it is useful for the rapid and differential diagnosis of HP-PRRSV JXA1-R, HP-PRRSV, and C-PRRSV.


Microbial Pathogenesis | 2017

The 1910HK/RR two-component system is essential for the virulence of Streptococcus suis serotype 2.

Fangyan Yuan; Chen Tan; Zewen Liu; Keli Yang; Danna Zhou; Wei Liu; Zhengying Duan; Rui Guo; Huanchun Chen; Yongxiang Tian; Weicheng Bei


Archive | 2012

Oligonucleotide primer pair, oligonucleotide composition, reagent kit comprising oligonucleotide composition and detection method of reagent kit

Keli Yang; Yongxiang Tian; Zhengying Duan; Zewen Liu; Rui Guo; Danna Zhou; Fangyan Yuan


Archive | 2012

ELISA (Enzyme-Linked Immunosorbent Assay) kit for detecting swine chlamydophila abortus antibody

Danna Zhou; Yongxiang Tian; Zewen Liu; Fangyan Yuan; Keli Yang; Zhengying Duan; Rui Guo


Archive | 2013

Effects of heavy metal pollution on microbial communities and activities of mining soils in Central Tibet, China

Danna Zhou; Fu-ping Zhang; Zhengying Duan; Zewen Liu; Keli Yang; Rui Guo; Fangyan Yuan; Yongxiang Tian; Cheng-fang Li

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Fangyan Yuan

Huazhong Agricultural University

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Huanchun Chen

Huazhong Agricultural University

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Rui Wu

Chinese Academy of Sciences

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Wan Liang

Huazhong Agricultural University

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Weicheng Bei

Huazhong Agricultural University

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Bin Wu

Huazhong Agricultural University

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BinWang

Huazhong Agricultural University

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Chen Tan

Huazhong Agricultural University

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Chengkun Zheng

Huazhong Agricultural University

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Hechao Zhu

Huazhong Agricultural University

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