Danny L. Lattin

Toxicity of methylenedioxymethamphetamine (MDMA) in the dog and the rat

Methylenedioxymethamphetamine (MDMA) was administered to dogs and rats orally once a day for a 28-day period to evaluate the morphological and neuropathological effects. Major clinical signs associated with the administration of MDMA in the dog included circling, depression, dilated pupils, hyperactivity, rapid breathing, and salivation. Major clinical signs in the rat included hyperactivity, excitability, piloerection, exophthalmos, and salivation. Gross observations at necropsy in the dog possibly related to administration of the test article included reduced testicular size (one high and one medium dose) and prostatic enlargement in two high-dose animals. No gross lesions were seen in the rats at necropsy. The medium- and the high-dose groups in both sexes in both the rats and the dogs gained significantly less weight than the control and low-dose groups. Food consumption decreased the first week for the high- and medium-dose groups, but a significant reversal toward more normal consumption was noted in the following weeks in both the rats and the dogs. Hematologic, clinical chemistry, and urinalysis values did not appear to be affected by the administration of the test article in the dog. In the rat clinical pathology variables showing a trend to decrease with dose included urinary pH, blood urea nitrogen, glucose, creatinine (females), lactate dehydrogenase (LDH) (females), and chloride. Clinical pathology variables showing a trend to increase with dose included total white blood cell count and phosphorus. Microscopically, testicular atrophy was present in one medium-dose and two high-dose male dogs. Prostatic hyperplasia was present in two high-dose male dogs. No test article-related lesions were seen in the brains of either species.

Laboratory to Laboratory Reproducibility of High Performance Liquid Chromatographic Retention Indices

Abstract Using seven typical drugs, the intra-and inter-laboratory reproducibility of the measured HPLC retention indices, relative retention times, and adjusted relative retention times using the same mobile phase and various reversed-phase C-18 columns were determined. Within a given laboratory, the respective relative standard deviations were ± 0.99%, ± 1.78%, and ± 2.63%. Between laboratories, the respective relative standard deviations were found to be ± 12.6%, ± 30.2%, and ± 34.8%. These results indicated that the HPLC retention index scale may be more useful in comparing data between laboratories.