Darci Pascoal Brasil

Estudos bacteriológicos e sorológicos de um surto de peste no Estado da Paraíba, Brasil

During a plaque outbreak in the Borborema Plateau focus (Paraiba), bacteriological and serological studies were carried out in material from 452 patients (48 positives), 1,938 rodents and other small mammals (75 positives), 4,756 dogs (141 positives) and 2,047 cats (57 positives) obtained from 41 counties (out of which, 21 produced positive samples). Twenty Yersinia pestis strains isolated from material from 3 patients and 17 rodents, displayed biochemical reactions, virulence factors, antibiotic susceptibility and animal experimental pathogenicity similar to those observed in strains previously isolated. According to our findings this recent plague outbreak did not exhibit different factors from those observed during prior outbreaks in other plague foci in the northeast of Brazil.During a plague outbreak in the Borborema Plateu focus (Paraiba), bacteriological and serological studies were carryed out in material from 452 patients (48 positives), 1,938 rodents and other small mammals (75 positives), 4,756 dogs (141 positives) and 2,047 cats (57 positives) obtained from 41 counties (out of which, 21 produced positive samples). Twenty Yersinia pestis strains isolated from material from 3 patients ans 17 rodents, displayed biochemical reactions, virulence factors, antibiotic susceptibility and animal experimental pathogenicity similar to those observed in strains previously isolated. According to our findings this recent plague outbreak did not exhibite different factors from those observed during prior outbreaks in other plague foci in the northeast of Brazil.

Isolamento da Yersinia pestis nos focos pestosos do Nordeste do Brasil no período de 1966 a 1982

From 1966 to 1982, 861 strains of Yersinia pestis were isolated from plague foci in Northeastern Brazil. The source of these isolates were: 471 rodents, 236 pools of fleas, 2 pools of Ornithodorus and 152 humans beings. Zygodontomys lasiurus pixuna was the rodent specie that produced mostly of the isolates as well as mostly of the infected pools of fleas mainly of the Polygenis species. The infected samples were originated from municipalities of Pernambuco (13) Ceara (7) ParaibaIII PiauiI and BahiaI States. This geographic distribution shows that plague remains a health problem in Brazil and deserves attention. The bigger numbers of isolates and affected areas in Pernambuco State does not necessarily indicates a higher infection incidence in such State but results from a more intense surveillance and skilled Laboratories for plague diagnosis.

Importância dos carnívoros domésticos (cães e gatos) na epidemiologia da peste nos focos do nordeste do Brasil

Soros de carnivoros domesticos (caes e gatos), roedores, e pacientes suspeitos de peste, obtidos durante dois surtos de peste humana e de roedores, um em 1982, no foco da Serra de Baturite/CE e o outro, em 1986, no Planalto da Borborema/PB e soros de carnivoros e de roedores do foco da Chapada do Araripe/PE, obtidos, em 1986, na ausencia de casos humanos, foram analisados pelas micro tecnicas de hemaglutinacao passiva para anticorpos especificos, contra a fracao antigenica purificada - F1A - de Yersinia pestis e inibicao da hemaglutinacao. Os altos percentuais de carnivoros, sorologicamente, positivos encontrados durante os surtos de peste humana, confirmam que esses animais estao implicados no ciclo epidemiologico da peste, nos focos do Nordeste do Brasil, e representam valiosos indicadores das atividades da infeccao.

Pesquisa da infecção natural por Yersinia pestis, em pulicídeos provenientes de focos pestosos do nordeste do Brasil

Three different containment transport processes of fleas were evaluated as an approach to the bacteriologic isolation of Yersinia pestis. The three methods employed were: live fleas in glass tubes containing pieces of wrapped filter paper; dead fleas in saline solution; and maceratedfleas in Cary-Blair culture medium. The two latter methods were almost equal and superior to the first method. A total of 29512 flea pools, from plague foci in Northeast Brazil collected during 1966 to 1982 were evaluated by the three methods. Among these samples, 236 (0.80%) flea pools were positive with regard to bacteriological cultivation and/or infection of susceptible animals.

Pesquisa de Yersinia pestis em roedores e outros pequenos mamíferos nos focos pestosos do Nordeste do Brasil no período 1966 a 1982

Foi feita analise da metodologia empregada e dos resultados alcancados em pesquisa de Yersinia pestis, em material de 24.703 roedores e outros pequenos mamiferos oriundos dos focos pestosos do Nordeste do Brasil, no periodo de 1966 a 1982. Concluiu-se ser necessario haver maior rapidez na realizacao dos exames para que os dados obtidos sejam convenientemente aplicados nas atividades de vigilância e controle da peste.Foi feita analise da metodologia empregada e dos resultados alcancados em pesquisa de Yersinia pestis, em material de 24.703 roedores e outros pequenos mamiferos oriundos dos focos pestosos do Nordeste do Brasil, no periodo de 1966 a 1982. Concluiu-se ser necessario haver maior rapidez na realizacao dos exames para que os dados obtidos sejam convenientemente aplicados nas atividades de vigilância e controle da peste.