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Dive into the research topics where Darío Andrinolo is active.

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Featured researches published by Darío Andrinolo.


Marine Drugs | 2011

An Acute Case of Intoxication with Cyanobacteria and Cyanotoxins in Recreational Water in Salto Grande Dam, Argentina

Leda Giannuzzi; Daniela Sedan; Ricardo O. Echenique; Darío Andrinolo

Cyanobacterial blooms and hepatotoxic microcystins (MCs) usually occur in summer, constituting a sanitary and environmental problem in Salto Grande Dam, Argentina. Water sports and recreational activities take place in summer in this lake. We reported an acute case of cyanobacterial poisoning in Salto Grande dam, Argentina, which occurred in January 2007. Accidentally, a young man was immersed in an intense bloom of Microcystis spp. A level of 48.6 μg·L−1 of microcystin-LR was detected in water samples. Four hours after exposure, the patient showed nausea, abdominal pain and fever. Three days later, dyspnea and respiratory distress were reported. The patient was hospitalized in intensive care and diagnosed with an atypical pneumonia. Finally, a week after the exposure, the patient developed a hepatotoxicosis with a significant increase of hepatic damage biomarkers (ALT, AST and γGT). Complete recovery took place within 20 days. This is the first study to show an acute intoxication with microcystin-producing cyanobacteria blooms in recreational water.


Toxicon | 2008

Hepatic recovery after damage produced by sub-chronic intoxication with the cyanotoxin microcystin LR

Darío Andrinolo; Daniela Sedan; Luis Telese; Claudia Aura; Silvia Masera; Leda Giannuzzi; Carlos Alberto Marra; María J.T. de Alaniz

The effect of sub-chronic exposure of intraperitoneal (i.p.) injections of microcystin-LR (MC-LR) on microscopic tissue architecture, hepatic function and lipid peroxidation has been studied in liver and kidney of mice. Mice were treated i.p. with 25 microg of pure MC-LR/kg body weight or saline solution for 1 month (every 2 days) with the aim of producing an inflictive stage with evident damage. Histopathological analysis of dissected livers of mice showed a disrupted lobar architecture and the development of cytoplasmatic vacuoles. According to this, a significant increase in hepatic lipid content and in lipid peroxidation levels in liver and kidney was found in MC-LR-treated animals when compared with controls. Moreover, serum alkaline phosphatase and aspartate aminotransferase activities showed a significant alteration in MC-LR-treated animals. After damage, progression or recovery was studied for 1 and 2 months of wash-out. The recovery from liver damage was evident at the cytological and physiological level, only the recovery of lobar architecture was incomplete along the period investigated. In conclusion, the present study demonstrates the ability of hepatic tissue to recover from damage produced by sub-chronic MC-LR administration. The dynamic interplay between damage and tissue-repairing response in determining the ultimate outcome of toxicity should be considered in risk-assessment studies.


Toxicon | 2010

Alteration and recovery of the antioxidant system induced by sub-chronic exposure to microcystin-LR in mice: Its relation to liver lipid composition

Daniela Sedan; Darío Andrinolo; Luis Telese; Leda Giannuzzi; María J.T. de Alaniz; Carlos Alberto Marra

The effects of MC-LR on antioxidant system in liver and kidney and its effects on hepatic lipid composition after prolonged exposure to sublethal doses of microcystins (MCs) were studied in mice. Mice were treated i.p. with 25 microg of MC-LR/kg body weight or saline solution every 2 days for a month (inflictive stage), then progression or recovery was studied for 1 and 2 months of wash-out. During the inflictive stage, MC-LR-induced oxidative damage and significant changes in liver lipids of treated mice were compared with control mice. A clear dependence of the enzyme defense system was demonstrated with reduced glutathione and alpha-tocopherol availabilities and a concomitant elevation in NOx production. Sub-chronic MC-LR toxicosis produced alterations in lipid components that included a decreased EFA/non-EFA, SFA/PUFA, and n-3/n-6 ratios all of which exhibited a pattern of slow recovery during the recovery periods.


Journal of Biochemical and Molecular Toxicology | 2012

Subchronic microcystin-LR exposure increased hepatic apoptosis and induced compensatory mechanisms in mice.

Noelia Lezcano; Daniela Sedan; Ignacio Lucotti; Leda Giannuzzi; Leticia Vittone; Darío Andrinolo; Cecilia Mundiña-Weilenmann

Acute lethal cytotoxicity of microcystin‐LR (MC‐LR), a toxin produced by fresh‐water cyanobacteria, has been attributed to protein phosphatases type 1 and type 2A (PP1/PP2A) inhibition and reactive oxygen species (ROS) generation. However, the effects and molecular mechanisms of prolonged, sublethal MC‐LR exposure are less known. We studied mice intraperitonealy injected with saline or 25 μg MC‐LR/kg for 28 days (every 2 days). MC‐LR induced apoptosis in liver and not in kidneys or heart of treated animals. Liver also showed decreased α‐tubulin levels (45.56% ± 7.65% of controls) and activation of p38‐MAPK and CaMKII pathways (137.93% ± 11.64% and 419.35% ± 67.83% of the control group, respectively). PP1/PP2A activity decreased from 1.82 ± 0.23 (controls) to 0.91 ± 0.98 mU/mg (MC‐LR–treated mice); however, no difference in total Ser/Thr phosphatase activity was found between both the groups. The results demonstrated that apoptosis and cytoskeleton disruption contributed to the hepatic cytotoxic effects of subchronic MC‐LR administration. These effects occurred in association with sustained activation of signaling cascades and development of compensatory mechanisms to maintain total Ser/Thr phosphatase activity.


Toxins | 2014

Application of Hydrogen Peroxide to the Control of Eutrophic Lake Systems in Laboratory Assays

Letizia Bauzá; Anabella Aguilera; Ricardo O. Echenique; Darío Andrinolo; Leda Giannuzzi

We exposed water samples from a recreational lake dominated by the cyanobacterium Planktothrix agardhii to different concentrations of hydrogen peroxide (H2O2). An addition of 0.33 mg·L−1 of H2O2 was the lowest effective dose for the decay of chlorophyll-a concentration to half of the original in 14 h with light and 17 h in experiments without light. With 3.33 mg·L−1 of H2O2, the values of the chemical oxygen demand (COD) decreased to half at 36 and 126 h in experiments performed with and without light, respectively. With increasing H2O2, there is a decrease in the total and faecal coliform, and this effect was made more pronounced by light. Total and faecal coliform were inhibited completely 48 h after addition of 3.33 mg·L−1 H2O2. Although the densities of cyanobacterial cells exposed to H2O2 did not decrease, transmission electron microscope observation of the trichomes showed several stages of degeneration, and the cells were collapsed after 48 h of 3.33 mg·L−1 of H2O2 addition in the presence of light. Our results demonstrate that H2O2 could be potentially used in hypertrophic systems because it not only collapses cyanobacterial cells and coliform bacteria but may also reduce chlorophyll-a content and chemical oxygen demand.


Toxicon | 2015

Hepatic and intestine alterations in mice after prolonged exposure to low oral doses of Microcystin-LR

Daniela Sedan; Martı́n R. Laguens; Guido Copparoni; Jorge Oswaldo Aranda; Leda Giannuzzi; Carlos Alberto Marra; Darío Andrinolo

Oral intake of Microcystin-LR (MC-LR) is the principal route of exposure to this toxin, with prolonged exposure leading to liver damage of unspecific symptomatology. The aim of the present paper was therefore to investigate the liver and intestine damage generated by prolonged oral exposure to low MC-LR doses (50 and 100 μg MC-LR/kg body weight, administrated every 48 h during a month) in a murine model. We found alterations in TBARS, SOD activity and glutathione content in liver and intestine of mice exposed to both doses of MC-LR. Furthermore, the presence of MC-LR was detected in both organs. We also found hepatic steatosis (3.6 ± 0.6% and 15.3 ± 1.6%) and a decrease in intraepithelial lymphocytes (28.7 ± 5.0% and 44.2 ± 8.7%) in intestine of 50- and 100-μg MC-LR/kg treated animals, respectively. This result could have important implications for mucosal immunity, since intraepithelial lymphocytes are the principal effectors of this system. Our results indicate that prolonged oral exposure at 50 μg MC-LR/kg every 48 h generates significant damage not only in liver but also in intestine. This finding calls for a re-appraisal of the currently accepted NOAEL (No Observed Adverse Effect Level), 40 μg MC-LR/kg body weight, used to derive the guideline value for MC-LR in drinking water.


Comparative Biochemistry and Physiology C-toxicology & Pharmacology | 2016

Growth, toxin production, active oxygen species and catalase activity of Microcystis aeruginosa (Cyanophyceae) exposed to temperature stress.

Leda Giannuzzi; Bernd Krock; Melina Celeste Crettaz Minaglia; Lorena Rosso; Christian Houghton; Daniela Sedan; Gabriela Malanga; Mariela Espinosa; Darío Andrinolo; Marcelo P. Hernando

Microcystis are known for their potential ability to synthesize toxins, mainly microcystins (MCs). In order to evaluate the effects of temperature on chlorophyll a (Chl a), growth, physiological responses and toxin production of a native Microcystis aeruginosa, we exposed the cells to low (23°C) and high (29°C) temperature in addition to a 26°C control treatment. Exponential growth rate was significantly higher at 29°C compared to 23°C and control, reaching 0.43, 0.32 and 0.33day(-)(1) respectively. In addition, there was a delay of the start of exponential growth at 23°C. However, the intracellular concentration of Chl a decreased significantly due to temperature change. A significant increase in intracellular ROS was observed in coincidence with the activation of enzymatic antioxidant catalase (CAT) during the first two days of exposure to 23° and 29°C in comparison to the control experiment, decreasing thereafter to nearly initial values. Five MCs were determined by LC-MS/MS analysis. In the experiments, the highest MC concentration, 205fg [Leu(1)] MC-LR.cell(-1) expressed as MC-LR equivalent was measured in the beginning of the experiment and subsequently declined to 160fg.cell(-1) on day 2 and 70fg.cell(-1) on day 4 in cells exposed to 29°C. The same trend was observed for all other MCs except for the least abundant MC-LR which showed a continuous increase during exposure time. Our results suggest a high ability of M. aeruginosa to perceive ROS and to rapidly initiate antioxidant defenses with a differential response on MC production.


Toxins | 2017

Recreational Exposure during Algal Bloom in Carrasco Beach, Uruguay: A Liver Failure Case Report

Flavia Vidal; Daniela Sedan; Daniel D Agostino; María Cavalieri; Eduardo Mullen; María Parot Varela; Cintia Flores; Josep Caixach; Darío Andrinolo

In January 2015, a 20-month-old child and her family took part in recreational activities at Carrasco and Malvín beaches (Montevideo, Uruguay). An intense harmful algae bloom (HAB) was developing along the coast at that time. A few hours after the last recreational exposure episode, the family suffered gastrointestinal symptoms which were self-limited except in the child’s case, who was admitted to hospital in Uruguay with diarrhea, vomiting, fatigue, and jaundice. The patient had increased serum levels of liver enzymes and bilirubin and five days later presented acute liver failure. She was referred to the Italian Hospital in Buenos Aires, being admitted with grade II–III encephalopathy and hepatomegaly and requiring mechanical respiratory assistance. Serology tests for hepatitis A, B, and C, Epstein-Barr virus, and cytomegalovirus were negative. Laboratory features showed anemia, coagulopathy, and increased serum levels of ammonium, alanine aminotransferase (ALT), aspartate aminotransferase (AST), and bilirubin. Autoimmune Hepatitis Type-II (AH-II) was the initial diagnosis based on a liver kidney microsomal type 1 antibodies (LKM-1) positive result, and twenty days later a liver transplant was performed. The liver histopathology had indicated hemorrhagic necrosis in zone 3, and cholestasis and nodular regeneration, which were not characteristic of AH-II. LC/ESI-HRMS (liquid chromatography electrospray ionization high-resolution mass spectrometry) analysis of MCs in the explanted liver revealed the presence of Microsytin-LR (MC-LR) (2.4 ng·gr−1 tissue) and [D-Leu1]MC-LR (75.4 ng·gr−1 tissue), which constitute a toxicological nexus and indicate a preponderant role of microcystins in the development of fulminant hepatitis.


Toxicon | 2013

Biomarkers of prolonged exposure to microcystin-LR in mice

Daniela Sedan; Leda Giannuzzi; Lorena Rosso; Carlos Alberto Marra; Darío Andrinolo


Acta toxicológica argentina | 2007

Occurrence of Microcystis aeruginosa and microcystins in Río de la Plata river (Argentina)

Darío Andrinolo; Paulo Pereira; Leda Giannuzzi; Claudia Aura; Silvia Massera; Mariela Caneo; Joseph Caixach; Mónica Barco; Ricardo O. Echenique

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Leda Giannuzzi

National University of La Plata

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Daniela Sedan

National University of La Plata

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Lorena Rosso

National University of La Plata

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Ricardo O. Echenique

National University of La Plata

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Carlos Alberto Marra

National University of La Plata

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Melina Celeste Crettaz Minaglia

National Scientific and Technical Research Council

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Gabriela Malanga

University of Buenos Aires

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Marcelo P. Hernando

National Scientific and Technical Research Council

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Cintia Flores

Spanish National Research Council

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Josep Caixach

Spanish National Research Council

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