Dariusz Sztafrowski

Effect of static magnetic field and Busulfan on HL-60 cell apoptosis

The article presents the results of the in vitro studies which were carried out to determine the effect of constant magnetic field (SMF static magnetic field) and organic chemical called Busulfan on the level of apoptosis of human leukemia cells HL-60 in the study populations. The mortality increase of cells HL-60 in the case of a constant magnetic field and busulfan with respect to groups of cells treated with cytostatic only has been discovered. The use of a constant magnetic field as a supporting factor in therapy with selected cytostatic has been proposed. Streszczenie. Artykuł prezentuje wyniki badań in vitro których przeprowadzenie miało na celu ustalenie wpływu stałego pola magnetycznego (SMF – ang. Static Magnetic Field) oraz związku chemicznego o nazwie Busulfan na poziom apoptozy komórek białaczki ludzkiej HL-60 w badanych populacjach. Stwierdzono wzrost śmiertelności komórek HL-60 w przypadku zastosowania stałego pola magnetycznego i Busulfanu względem grupy komórek poddanych działaniu samego cytostatyku. Postuluje się zastosowanie stałego pola magnetycznego jako czynnika wspomagającego terapię wybranymi cytostatykami. (Wpływ stałego pola magnetycznego oraz busulfanu na apoptozę komórek HL-60)

Measuring analysis of the distribution of magnetic field free of errors resulting from fluctuation of currents

The paper presents a new method for measuring the electromagnetic field distribution under power transmission lines in real field conditions that is free from error brought by fluctuations of voltage, current and/or deviation of the multiphase system. The article analyses these problems for a selected case of the 110 kV line. Streszczenie. W artykule przedstawiono nową metodę pomiaru rozkładu składowej magnetycznej pola elektromagnetycznego pod liniami elektroenergetycznymi, która jest wolna od błędów wnoszonych przez wahania napięć, prądów i/lub odchyłki symetrii faz układu wielofazowego. W artykule przeanalizowano powyższe problemy dla wybranej linii 110 kV.

Ograniczenie natężenia pola elektrycznego pod linią napowietrzną 110 kV poprzez dedykowaną konfigurację geometryczną przewodów roboczych

The global value of the electric field intensity near overhead power lines is the superposition of the components of the electrical field generated by each of the phase wires of the power line. Therefore, the use of planned geometrical configuration of the phase wires can result, to some extent, in mutual compensation of component fields what reduces the intensity of global electric field generated by the overhead power lines. (Minimization of electric field intensity of 110 kV overhead power lines by purposeful configuration of phase wires). Słowa kluczowe: linie wysokiego napięcia, pole elektryczne, symulacje cyfrowe, metoda elementów skończonych, ochrona środowiska.

Electromagnetic Field Seems to Not Influence Transcription via CTCT Motif in Three Plant Promoters

It was proposed that magnetic fields (MFs) can influence gene transcription via CTCT motif located in human HSP70 promoter. To check the universality of this mechanism, we estimated the potential role of this motif on plant gene transcription in response to MFs using both bioinformatics and experimental studies. We searched potential promoter sequences (1000 bp upstream) in the potato Solanum tuberosum and thale cress Arabidopsis thaliana genomes for the CTCT sequence. The motif was found, on average, 3.6 and 4.3 times per promoter (148,487 and 134,361 motifs in total) in these two species, respectively; however, the CTCT sequences were not randomly distributed in the promoter regions but were preferentially located near the transcription initiation site and were closely packed. The closer these CTCT sequences to the transcription initiation site, the smaller distance between them in both plants. One can assume that genes with many CTCT motifs in their promoter regions can be potentially regulated by MFs. To check this assumption, we tested the influence of MFs on gene expression in a transgenic potato with three promoters (16R, 20R, and 5UGT) containing from 3 to 12 CTCT sequences and starting expression of β-glucuronidase as a reported gene. The potatoes were exposed to a 50 Hz 60–70 A/m MF for 30 min and the reporter gene activity was measured for up to 24 h. Although other factors induced the reporter gene activity, the MF did not. It implies the CTCT motif does not mediate in response to MF in the tested plant promoters.