Darja Matt

Beetroot (Beta vulgaris L.) and naturally fermented beetroot juices from organic and conventional production: metabolomics, antioxidant levels and anticancer activity

BACKGROUND The aim of the paper was to determine the level of antioxidants and metabolomic fingerprinting in both raw beetroots and naturally fermented beetroot juices from organic (ORG) versus conventional (CONV) production. In addition, the anticancer properties of the fermented beetroot juices were evaluated. RESULTS The obtained results showed that ORG fresh beetroots contained significantly more dry matter, vitamin C and some individual phenolic compounds than CONV beetroots. The content of total phenolic acids was significantly higher in CONV beetroots compared with the ORG ones. The level of flavonoids was similar in ORG and CONV beetroots. There were only slight differences in the chemical composition of ORG and CONV beetroot juices. Metabolomic analysis provided a possibility to distinguish clearly between ORG and CONV fermented beetroot juices. However, this method was less useful in the case of fresh whole beetroots. It was found that anticancer activity was stronger in the case of ORG fermented juices when compared with CONV ones. CONCLUSION The obtained results indicate that ORG- and CONV-produced beetroots and fermented beetroot juices have different chemical properties and different impacts on cancer cells. It is necessary to continue research on this topic in order to confirm and understand the achieved results.

How organic farmers practice conservation agriculture in Europe

The interest of organic farmers in adopting conservation agriculture principles, including minimal soil disturbance, permanent soil cover and crop rotation has been growing since the early 2000s. However, currently there is no network for organic farmers practicing conservation agriculture, and a lack of knowledge on how organic farmers implement conservation agriculture in practice. Consequently, few technical references are available for organic farmers when they start applying conservation agriculture practices, in particular on controlling weeds without the use of herbicides. The main objectives of this study were: (1) to explore the diversity of conservation agriculture techniques (i.e., reduced tillage, no-tillage and green manures) practiced among European farmers, and (2) to identify farmers’ main strategies for implementing conservation agriculture and the agronomic and environmental factors that determine these strategies. Strategies were identified by analyzing survey results on: (1) the type and degree of use of conservation agriculture practices by farmers, and (2) the effects it produces in terms of soil disturbance and soil cover (low, medium and high). We carried out a survey of 159 European organic farmers and collected 125 data sets on management of winter-sown crops. Among the conservation agriculture practices, reduced tillage was used by 89%, no-tillage by 27% and green manure by 74% of the 159 interviewed farmers. Green manures were more frequently used in northern Europe than in the south (below 45°N). Most of the farmers used crop rotations, with a mean duration of 6 years. A wide diversity of conservation agriculture practices were used, with farmers rarely using all three techniques (no-till, reduced till and green manures) within one system. The range of practices was grouped into five strategies ranging from intensive non-inversion tillage without soil cover to very innovative techniques with no-tillage and intercrops. The five strategies for conservation agriculture could be grouped into two larger categories based on weed control approach: (1) intensification of the mechanical work without soil inversion or (2) biological regulation of weeds with cover crops. The diversity of strategies identified in this study shows that organic farmers use innovative approaches to implement conservation agriculture without herbicides. This studys findings will help organic farmers to experiment with innovative practices based on conservation agriculture principles and also benefit conventional farmers who use conservation agriculture practices and would like to reduce or eliminate the use of herbicides.

How the organic food system supports sustainable diets and translates these into practice

Organic production and consumption provide a delineated food system that can be explored for its potential contribution to sustainable diets. While organic agriculture improves the sustainability performance on the production side, critical reflections are made on how organic consumption patterns, understood as the practice of people consuming significant amounts of organic produce, may also be taken as an example for sustainable food consumption. The consumption patterns of regular organic consumers seem to be close to the sustainable diet concept of FAO. Certain organic-related measures might therefore be useful in the sustainability assessment of diets, e.g., organic production and organic consumption. Since diets play a central role in shaping food systems and food systems shape diets, the role of organic consumption emerges as an essential topic to be addressed. This role may be based on four important organic achievements: organic agriculture and food production has a definition, well-established principles, public standards, and useful metrics. By 2015, data for organic production and consumption are recorded annually from more than 160 countries, and regulations are in force in more than 80 countries or regions. The organic food system puts the land (agri-cultura) back into the diet; it is the land from which the diet in toto is shaped. Therefore, the organic food system provides essential components of a sustainable diet.

Three-Year Comparative Study of Polyphenol Contents and Antioxidant Capacities in Fruits of Tomato (Lycopersicon esculentum Mill.) Cultivars Grown under Organic and Conventional Conditions

In the present study, four tomato cultivars were grown under organic and conventional conditions in separate unheated greenhouses in three consecutive years. The objective was to assess the influence of the cultivation system on the content of individual polyphenols, total phenolics, and antioxidant capacity of tomatoes. The fruits were analyzed for total phenolic content by the Folin-Ciocalteau method and antioxidant capacity by the DPPH free radical scavenging assay. Individual phenolic compounds were analyzed using HPLC-DAD-MS/MS. Among 30 identified and quantified polyphenols, significantly higher contents of apigenin acetylhexoside, caffeic acid hexoside I, and phloretin dihexoside were found in all organic samples. The content of polyphenols was more dependent on year and cultivar than on cultivation conditions. Generally, the cultivation system had minor impact on polyphenols content, and only a few compounds were influenced by the mode of cultivation in all tested cultivars during all three years.

Pesticide residues in Estonian local and imported food in 2008–2011

Abstract The use of synthetic pesticides is very common in conventional agriculture as plant protection measures and for improving storage of food. For increasing food safety, monitoring programmes are taking place in each European Union Member State and in Norway and Iceland every year. The monitoring aims to detect samples from food exceeding the maximum residue level (MRL) and provides data for an assessment of consumer exposure to pesticide residues. Results from Estonian national monitoring programme conducted by Veterinary and Food Board are presented in this paper, used to find out the differences in pesticide residue occurrences and MRL exceedances in local and imported food consumed in Estonia. In 2008, residues of one or more pesticides were found in 35–53% of the total analysed samples of fruits, vegetables and cereals. Imported food contained more pesticide residues compared to local food. MRLs were exceeded by 1.6% in 2008 and 2.3% in 2009 out of all samples, residues exceeding the MRLs were found in imported samples only. In 2010, 2.1% of total samples were above MRL. In 2011, 0.7% of total samples exceeded MRL which were of domestic origin. The highest MRL exceedances from the years 2008 to 2011 were propargite in beans with pods from Estonia over 10 times, chlormequat in grapes from India 7.9 times and trifluralin in tea from China 7.5 times. MRL was most frequently exceeded by dithiocarbamates, in 12 cases out of 22. The highest number of different pesticide residues in a single sample was 10 in apple from Belgium, 9 in mandarin from South Africa, 8 in strawberries from Spain and in apple from Poland. The results of monitoring programme show that local food contains less pesticide residues than imported food. By consuming food locally, it is possible to reduce the consumption of pesticide residues.

The Nutritive Value of Organic and Conventional White Cabbage (Brassica Oleracea L. Var. Capitata) and Anti-Apoptotic Activity in Gastric Adenocarcinoma Cells of Sauerkraut Juice Produced Therof

White cabbage is one of the most important vegetables grown both in Poland and worldwide. Cabbage contains considerable amounts of bioactive compounds such as glucosinolates, vitamin C, carotenoids, and polyphenols. Some experiments indicate that vegetables from organic production contain more bioactive compounds than those from conventional production, however, only a few studies have been conducted on cruciferous plants. The presented study has proved that organic fresh cabbage, compared to the conventional one, contained significantly less total flavonoids in both years of experiments (3.95 ± 0.21 mg/100 g FW and 3.71 ± 0.33 mg/100 g FW), several flavonoid compounds, total chlorophylls (1.51 ± 0.17 mg/100 g FW and 1.30 ± 0.22 mg/100 g FW) carotenoids, nitrites (0.55 ± 0.04 mg/kg FW and 0.45 ± 0.02 mg/kg FW), and nitrates (0.50 ± 0.13 g/kg FW and 0.47 ± 0.11 g/kg FW). The organic sauerkraut juice, compared to the conventional one, contained significantly more total polyphenols (5.39 ± 0.22 mg/100 g FW and 9.05 ± 1.10 mg/100 g FW) as well as several flavonoids. Only CONV sauerkraut juice produced with the highest N level of fertilization induced a statistical significant increase of the level of necrosis of human stomach gastric adenocarcinoma cell line AGS.

Diversity of Conservation Agriculture Practices Among European Organic farmers

The interest of organic farmers for adopting conservation agriculture practices is currently growing. But, there are few technical and scientific references available for organic farmers when they start applying conservation agriculture practices. The main objectives of this study are (1) to explore the diversity of crop management practices using conservation agriculture methods among European farmers, and (2) to identify main farmers’ strategies. We carried out a survey of 159 farmers located in 10 European countries. Data were analysis to identify groups of farmers that share the same type of spring and winter crop managements. Organic farmers in Europe show very diverse crop management. The high diversity of crop management can be described by two main strategies: ‘low soil cover’ and ‘soil conservation’ strategy. Distinct geographical distribution of both strategies suggests that applicability of conservation agriculture practices is strongly context oriented.Conserving and improving the fertility and quality of the limited soil resource to produce food, feed and fibre has always been the key to organic farmers’ management practices. This issue is also addressed in conservation agriculture systems that give up on soil tillage (no-tillage) or reduce tillage intensity, but often build on the extensive use of herbicides and mineral fertilizers. Both systems show advantages for soil quality (Holland, 2004; Mader et al., 2002) and therefore their combination is promising and may provide better soil quality. Challenges of introducing noor reduced tillage systems into organic farming are increased weed pressure, retarded mineralization of nutrients that both may lead to reduced crop yield (Peigne et al., 2007). Pioneer farmers have developed solutions and new machinery to be applicable in organic farming systems. Comparisons of reduced tillage to the traditional plough system have started on farms and systematic research started a decade ago. It was the aim of our research activities, accomplished within the frame of the European network TILMAN-ORG (www.tilman-org.net), to evaluate changes in soil carbon stocks and biological soil fertility parameters in soils from European field trials that compared reduced primary soil tillage options with standard procedures (mainly plough). The selected sites represent a geoclimatic gradient from the North-East to the South-West. The hypothesis was that reduced tillage is enhancing the stratification of soil organic matter, soil microbial biomass and activity, and is changing microbial community structure and microbial functions.Conservation agriculture and organic farming are currently considered as environmentally friendly options for producing food. This study explores the motivations and problems of organic European farmers that apply at least two conservation techniques: (i) no-tillage, (ii) reduced tillage and/or (iii) green manure. We carried out a survey with 159 farmers located in 10 European countries. Data were analysed with a principal component analysis followed by clustering to identify groups of farmers with similar motivations and problems. The most important motivations are related to soil preservation and problems are mainly linked to agronomic conditions and crop management. There are three groups of farmers that share the same type of attitude: “atypical farmers”, “soil conservationists” and “agro-technically challenged farmers”. Further research may address in priority agronomic problems, such as weed infestation, caused by adoption of conservation agriculture in organic agriculture.Environmental concerns are pushing organic farmers to substitute ploughing with reduced tillage but weed management under reduced tillage can be troublesome. One objective of the TILMAN-ORG Project is to improve weed management with reduced tillage ± cover crops without reducing weed community diversity. This paper reports results obtained in 2012 in 13 long-, mid-, and short-term trials scattered across Europe including winter cereals, maize, grain pea, potato, sunflower and grass/clover or legume leys. Longand midterm experiments showed higher weed abundance under reduced tillage but usually without reduced crop yield. Short-term trials showed that reduced tillage system may be troublesome to manage right after the conversion from ploughing, due to higher abundance of weeds and volunteer crops and reduced yield. Interestingly, there was no overall consistent relationship between weed diversity, always higher under reduced tillage, and crop yield.