Darlene S. S. Lim

Constraining carbon sources and growth rates of freshwater microbialites in Pavilion Lake using 14C analysis

This study determined the natural abundance isotopic compositions ((13)C, (14)C) of the primary carbon pools and microbial communities associated with modern freshwater microbialites located in Pavilion Lake, British Columbia, Canada. The Delta(14)C of dissolved inorganic carbon (DIC) was constant throughout the water column and consistent with a primarily atmospheric source. Observed depletions in DIC (14)C values compared with atmospheric CO(2) indicated effects due either to DIC residence time and/or inputs of (14)C-depleted groundwater. Mass balance comparisons of local and regional groundwater indicate that groundwater DIC could contribute a maximum of 9-13% of the DIC. (14)C analysis of microbial phospholipid fatty acids from microbialite communities had Delta(14)C values comparable with lake water DIC, demonstrating that lake water DIC was their primary carbon source. Microbialite carbonate was also primarily derived from DIC. However, some depletion in microbialite carbonate (14)C relative to lake water DIC occurred, due either to residence time or mixing with a (14)C-depleted carbon source. A detrital branch covered with microbialite growth was used to estimate a microbialite growth rate of 0.05 mm year(-1) for the past 1000 years, faster than previous estimates for this system. These results demonstrate that the microbialites are actively growing and that the primary carbon source for both microbial communities and recent carbonate is DIC originating from the atmosphere. While these data cannot conclusively differentiate between abiotic and biotic formation mechanisms, the evidence for minor inputs of groundwater-derived DIC is consistent with the previously hypothesized biological origin of the Pavilion Lake microbialites.

Limnology of Pavilion Lake, B. C., Canada - characterization of a microbialite forming environment.

The objectives of this study are two-fold: (1) to describe and quantify the seasonal physical and chemical limnological properties of Pavilion Lake, a microbialite-rich lake in British Columbia, Canada, and (2) to gain a broader limnological context of Pavilion Lake by examining the limnology and hydrology of the lakes and groundwater systems in the area (∼30 km radius). Pavilion Lake is a dimictic lake with annual ice-cover. It is a hard water (mean CaCO 3 = 181.8 mg L -1), ultra-oligotrophic (mean Total Phosphorus [TP] = 3.3 μg L -1) lake, that is groundwater fed most likely through diffuse, low velocity local and regional sources. Principal Components Analysis (PCA) separated Pavilion Lake from the other groundwater, stream and lake samples along a conductivity and pH gradient on Axis 1 (λ 1 =0.392), and a nutrient (Total Nitrogen [TN], TP) and K +, Mg 2+, Si gradient on the second axis (λ 2 = 0.160). Pavilion Lake has the lowest Ca 2+ and TP concentrations, and the highest Na + concentrations and optical transmissivity amongst all sampled sites. Furthermore, the lake is characterized by low sedimentation rates. These characteristics are potentially important factors in supporting the past and on-going development of microbialites within the lake. Our study provides a limnochemical reference to consider in the conceptualization of ideal environments supporting large-scale microbialites.

Metagenomic Analysis Suggests Modern Freshwater Microbialites Harbor a Distinct Core Microbial Community

Modern microbialites are complex microbial communities that interface with abiotic factors to form carbonate-rich organosedimentary structures whose ancestors provide the earliest evidence of life. Past studies primarily on marine microbialites have inventoried diverse taxa and metabolic pathways, but it is unclear which of these are members of the microbialite community and which are introduced from adjacent environments. Here we control for these factors by sampling the surrounding water and nearby sediment, in addition to the microbialites and use a metagenomics approach to interrogate the microbial community. Our findings suggest that the Pavilion Lake microbialite community profile, metabolic potential and pathway distributions are distinct from those in the neighboring sediments and water. Based on RefSeq classification, members of the Proteobacteria (e.g., alpha and delta classes) were the dominant taxa in the microbialites, and possessed novel functional guilds associated with the metabolism of heavy metals, antibiotic resistance, primary alcohol biosynthesis and urea metabolism; the latter may help drive biomineralization. Urea metabolism within Pavilion Lake microbialites is a feature not previously associated in other microbialites. The microbialite communities were also significantly enriched for cyanobacteria and acidobacteria, which likely play an important role in biomineralization. Additional findings suggest that Pavilion Lake microbialites are under viral selection as genes associated with viral infection (e.g CRISPR-Cas, phage shock and phage excision) are abundant within the microbialite metagenomes. The morphology of Pavilion Lake microbialites changes dramatically with depth; yet, metagenomic data did not vary significantly by morphology or depth, indicating that microbialite morphology is altered by other factors, perhaps transcriptional differences or abiotic conditions. This work provides a comprehensive metagenomic perspective of the interactions and differences between microbialites and their surrounding environment, and reveals the distinct nature of these complex communities.

Planetary science and exploration in the deep subsurface : results from the MINAR Program, Boulby Mine, UK

The authors would also like to acknowledge the funding provided by the STFC Impact Acceleration Fund. Claire R. Cousins is supported by a Royal Society of Edinburgh Research Fellowship. The development of the ExoMars PanCam, the AUPE2 system and the PanCam data processing pipeline has been supported by funding from the UK Space Agency (lead funding agency) and the European Community’s Seventh Framework Program.

Multiplatform ocean exploration: Insights from the NEEMO space analog mission

Since the beginning of space exploration, methods and protocols of exploration have been developed using space analogs on Earth to reduce research costs, develop safe deployment/retrieval protocols, and ready astronauts for hostile environments in less threatening settings. Space analogs are required as much as ever today as astronauts and scientists develop new tools and techniques for exploration, while working to address evolving mission objectives from low-earth orbit to deepspace exploration. This study examines coordinated human and robotic exploration at the Aquarius Underwater Habitat off of the coast of Key Largo, Florida, in support of the NEEMO 15 (NASA Extreme Environment Mission Operations) program. The exploration scheme presented in this work fuses (1) robotic precursormissions as a means of remote sensing data collection; (2) crowdsourcing to process immense amounts of data to identify key targets of interest that might be missed in the tight cycle of mission operations; and (3) human exploration to examine locations directly up close and collect physical samples that require involved sampling techniques. Autonomous underwater vehicles (AUVs) and single-person submersibles, called DeepWorkers™, were used as underwater analogs of robotic systems currently being used and human-operated vehicles (HOVs) proposed for use on a Near Earth Asteroid (NEA), the Moon, or Mars. In addition to operational lessons learned for space exploration that are directly applicable to ocean exploration, ocean floor mapping provides new levels of detail of benthic habitat critical for coral reef monitoring and management. Opportunistic (onsite adaptive) data sampling also took place by placing self-recording instrumentation onto each of the DeepWorkers, increasing the collection of scientific information during the submersible missions and contributing to mission planning for optimal and efficient use of expensive assets.

Extravehicular activity operations concepts under communication latency and bandwidth constraints

The Biologic Analog Science Associated with Lava Terrains (BASALT) project is a multi-year program dedicated to iteratively develop, implement, and evaluate concepts of operations (ConOps) and supporting capabilities intended to enable and enhance human scientific exploration of Mars. This paper describes the planning, execution, and initial results from the first field deployment, referred to as BASALT-1, which consisted of a series of ten simulated extravehicular activities on volcanic flows in Idahos Craters of the Moon National Monument and Preserve. The ConOps and capabilities deployed and tested during BASALT-1 were based on previous NASA trade studies and analog testing. Our primary research question was whether those ConOps and capabilities work acceptably when performing real (non-simulated) biological and geological scientific exploration under four different Mars-to-Earth communication conditions: 5 and 15 min one-way light time communication latencies and low (0.512 Mb/s uplink, 1.54 Mb/s downlink) and high (5.0 Mb/s uplink, 10.0 Mb/s downlink) bandwidth conditions, which represent two alternative technical communication capabilities currently proposed for future human exploration missions. The synthesized results, based on objective and subjective measures, from BASALT-1 established preliminary findings that the baseline ConOp, software systems, and communication protocols were scientifically and operationally acceptable with minor improvements desired by the “Mars” extravehicular and intravehicular crewmembers. However, unacceptable components of the ConOps and required improvements were identified by the “Earth” Mission Support Center. These data provide a basis for guiding and prioritizing capability development for future BASALT deployments and, ultimately, future human exploration missions.

Paleolimnology in the High Arctic - implications for the exploration of Mars

Paleolimnology provides information on the past chemical, physical and biological nature of water bodies. In polar regions, where global climatic changes can be exacerbated compared with lower latitudes, the science has become important for reconstructing past changes and in so doing, predicting possible effects of future changes. Owing to the association of life with water bodies, particularly stable water bodies sustained over many millennia, paleolake regions on the surface of Mars are of exobiological importance. In this mini-review, we use experience gathered in the High Arctic to describe the importance of paleolimnology in the Earths polar regions as it pertains to the future application of this science to robotic and human exploration missions to the planet Mars

Remote Physiological Monitoring in a Mars Analog Field Setting

Abstract The ability to remotely monitor physiological parameters of individuals while working in an extreme environment is useful for promoting health and safety. There is limited evidence of real-time, remote ambulatory physiological monitoring during field science tasks with readily available, Commercial-Off-The-Shelf (COTS) products. The Zephyr BioHarnessTM was integrated into NASA’s BASALT Mars Analog field deployment for real-time, remote physiological monitoring of extravehicular crew during tasks approximating those that will be performed during planetary exploration (field traverses and sample collection). This project was included in BASALT to assess the feasibility of using COTS physiological monitors to stream live data and develop protocols for real-time health status determination under remote networking conditions in a field geology setting. Physiological monitoring data were transmitted from a field setting to a remote monitoring site at 1 Hz resolution in 23 out of 28 simulated EVAs. Outages were generally attributed to the difficulties of implementing a field network in an environment such as the ones in which BASALT operated, rather than inherent issues with the COTS instrumentation. Results of this study indicate that this application of COTS physiological monitoring is potentially applicable to a range of field research tasks.

The Complete Genome and Physiological Analysis of the Microbialite-Dwelling Agrococcus pavilionensis sp. nov; Reveals Genetic Promiscuity and Predicted Adaptations to Environmental Stress

Members of the bacterial genus Agrococcus are globally distributed and found across environments so highly diverse that they include forests, deserts, and coal mines, as well as in potatoes and cheese. Despite how widely Agrococcus occurs, the extent of its physiology, genomes, and potential roles in the environment are poorly understood. Here we use whole-genome analysis, chemotaxonomic markers, morphology, and 16S rRNA gene phylogeny to describe a new isolate of the genus Agrococcus from freshwater microbialites in Pavilion Lake, British Columbia, Canada. We characterize this isolate as a new species Agrococcus pavilionensis strain RW1 and provide the first complete genome from a member of the genus Agrococcus. The A. pavilionensis genome consists of one chromosome (2,627,177 bp) as well as two plasmids (HC-CG1 1,427 bp, and LC-RRW783 31,795 bp). The genome reveals considerable genetic promiscuity via mobile elements, including a prophage and plasmids involved in integration, transposition, and heavy-metal stress. A. pavilionensis strain RW1 differs from other members of the Agrococcus genus by having a novel phospholipid fatty acid iso-C15:1Δ4, β-galactosidase activity and amygdalin utilization. Carotenoid biosynthesis is predicted by genomic metabolic reconstruction, which explains the characteristic yellow pigmentation of A. pavilionensis. Metabolic reconstructions of strain RW1 genome predicts a pathway for releasing ammonia via ammonification amino acids, which could increase the saturation index leading to carbonate precipitation. Our genomic analyses suggest signatures of environmental adaption to the relatively cold and oligotrophic conditions of Pavilion Lake microbialites. A. pavilionensis strain RW1 in modern microbialites has an ecological significance in Pavilion Lake microbialites, which include potential roles in heavy-metal cycling and carbonate precipitation (e.g., ammonification of amino acids and filamentation which many trap carbonate minerals).

BASALT A: Basaltic Terrains in Idaho and Hawaii as Planetary Analogs for Mars Geology and Astrobiology

Abstract Field research target regions within two basaltic geologic provinces are described as Earth analogs to Mars. Regions within the eastern Snake River Plain of Idaho and the Big Island of Hawai‘i, the United States, provinces that represent analogs of present-day and early Mars, respectively, were evaluated on the basis of geologic settings, rock lithology and geochemistry, rock alteration, and climate. Each of these factors provides rationale for the selection of specific targets for field research in five analog target regions: (1) Big Craters and (2) Highway lava flows at Craters of the Moon National Monument and Preserve, Idaho, and (3) Mauna Ulu low shield, (4) Kīlauea Iki lava lake, and (5) Kīlauea caldera in the Kīlauea Volcano summit region and the East Rift Zone of Hawai‘i. Our evaluation of compositional and textural attributes, as well as the effects of syn- and posteruptive rock alteration, shows that basaltic terrains in Idaho and Hawai‘i provide a way to characterize the geology and major geologic substrates that host biological activity of relevance to Mars exploration. This work provides the foundation to better understand the scientific questions related to the habitability of basaltic terrains, the rationale behind selecting analog field targets, and their applicability as analogs to Mars.