David A. Boulton

Microchemical structural determination of a peptoid covalently bound to a polymeric bead by matrix-assisted laser desorption ionization time-of-flight mass spectrometry

Abstract New methodology utilizing matrix-assisted laser desorption ionization time-of-flight mass spectroscopy (MALDI-TOF MS) for the direct identification of a ligand covalently attached to a polymeric bead is reported. The method allows for structure determination without relying on encoding strategies. Gaseous TFA cleavage of a peptoid from a single bead followed by mounting in MALDI matrix and irradiation with a beam at 337 nm gave abundant molecular ions and structurally informative fragment ions produced by post source decay (PSD).