David A. Fielder

Isolation of 4 -Acetyl-benzoxazolin-2-one (4-ABOA) and Diferuloylputrescine from an Extract of Gibberella Ear Rot-resistant Corn that Blocks Mycotoxin Biosynthesis, and the Insect Toxicity of 4 -ABOA and Related Compounds

Abstract Bioassay-directed fractionation of an extract of a gibberella ear rot-resistant corn hybrid resulted in the isolation of a fraction that inhibited mycotoxin production, but not growth, of Fusarium culmorum. The fraction contained diferuloylputrescine and a new allelochemical 4-acetyl-benzoxazolin-2-one (4-ABOA). This report describes the isolation of the bioactive fraction and tests of the effect of 4-ABOA on 3-acetyl deoxynivalenol (3ADON) and aflatoxin production. In addition, data are reported on the effects of 4-ABOA and related compounds on a number of insect species and some preliminary studies on the mechanism of insect toxicity. The effect of 4-ABOA on F. culmorum and Apergillus flavus was similar. Production of toxin was reduced without a concomitant reduction of growth. A 50% inhibition of 3ADON production occurred at ca. 4 μM and 0.12 mM for alfatoxin. 4-ABOA was slightly toxic to Spodoptera frugiperda.

Isolation and characterization of 4-acetyl-benzoxazolin-2-one (4-ABOA), a new benzoxazolinone from Zea mays

Abstract The previously unreported 4-acetyl-benzoxazolin-2-one (4-ABOA, 1 ) was isolated from corn kernels. Its structure was determined by MS, NMR and X-Ray crystallography.