Network


Latest external collaboration on country level. Dive into details by clicking on the dots.

Hotspot


Dive into the research topics where David Caballero is active.

Publication


Featured researches published by David Caballero.


Analytica Chimica Acta | 2012

Impedimetric immunosensor for human serum albumin detection on a direct aldehyde-functionalized silicon nitride surface.

David Caballero; Elena Martínez; J. Bausells; Abdelhamid Errachid; J. Samitier

In this work we report the fabrication and characterization of a label-free impedimetric immunosensor based on a silicon nitride (Si(3)N(4)) surface for the specific detection of human serum albumin (HSA) proteins. Silicon nitride provides several advantages compared with other materials commonly used, such as gold, and in particular in solid-state physics for electronic-based biosensors. However, few Si(3)N(4)-based biosensors have been developed; the lack of an efficient and direct protocol for the integration of biological elements with silicon-based substrates is still one of its the main drawbacks. Here, we use a direct functionalization method for the direct covalent binding of monoclonal anti-HSA antibodies on an aldehyde-functionalized Si-p/SiO(2)/Si(3)N(4) structure. This methodology, in contrast with most of the protocols reported in literature, requires less chemical reagents, it is less time-consuming and it does not need any chemical activation. The detection capability of the immunosensor was tested by performing non-faradaic electrochemical impedance spectroscopy (EIS) measurements for the specific detection of HSA proteins. Protein concentrations within the linear range of 10(-13)-10(-7) M were detected, showing a sensitivity of 0.128 Ω μM(-1) and a limit of detection of 10(-14) M. The specificity of the sensor was also addressed by studying the interferences with a similar protein, bovine serum albumin. The results obtained show that the antibodies were efficiently immobilized and the proteins detected specifically, thus, establishing the basis and the potential applicability of the developed silicon nitride-based immunosensor for the detection of proteins in real and more complex samples.


ACS Nano | 2018

Directed Flow of Micromotors through Alignment Interactions with Micropatterned Ratchets

Jaideep Katuri; David Caballero; Raphaël Voituriez; J. Samitier; Samuel Sanchez

To achieve control over naturally diffusive, out-of-equilibrium systems composed of self-propelled particles, such as cells or self-phoretic colloids, is a long-standing challenge in active matter physics. The inherently random motion of these active particles can be rectified in the presence of local and periodic asymmetric cues given that a nontrivial interaction exists between the self-propelled particle and the cues. Here, we exploit the phoretic and hydrodynamic interactions of synthetic micromotors with local topographical features to break the time-reversal symmetry of particle trajectories and to direct a macroscopic flow of micromotors. We show that the orientational alignment induced on the micromotors by the topographical features, together with their geometrical asymmetry, is crucial in generating directional particle flow. We also show that our system can be used to concentrate micromotors in confined spaces and identify the interactions leading to this effect. Finally, we develop a minimal model, which identifies the key parameters of the system responsible for the observed rectification. Overall, our system allows for robust control over both temporal and spatial distribution of synthetic micromotors.


ACS Applied Materials & Interfaces | 2017

Topological control of extracellular matrix growth: a native-like model for cell morphodynamics studies

David Caballero; J. Samitier

The interaction of cells with their natural environment influences a large variety of cellular phenomena, including cell adhesion, proliferation, and migration. The complex extracellular matrix network has challenged the attempts to replicate in vitro the heterogeneity of the cell environment and has threatened, in general, the relevance of in vitro studies. In this work, we describe a new and extremely versatile approach to generate native-like extracellular matrices with controlled morphologies for the in vitro study of cellular processes. This general approach combines the confluent culture of fibroblasts with microfabricated guiding templates to direct the three-dimensional growth of well-defined extracellular networks which recapitulate the structural and biomolecular complexity of features typically found in vivo. To evaluate its performance, we studied fundamental cellular processes, including cell cytoskeleton organization, cell-matrix adhesion, proliferation, and protrusions morphodynamics. In all cases, we found striking differences depending on matrix architecture and, in particular, when compared to standard two-dimensional environments. We also assessed whether the engineered matrix networks influenced cell migration dynamics and locomotion strategy, finding enhanced migration efficiency for cells seeded on aligned matrices. Altogether, our methodology paves the way to the development of high-performance models of the extracellular matrix for potential applications in tissue engineering, diagnosis, or stem-cell biology.


Cell Adhesion & Migration | 2015

Foreword: Physics of cell migration

David Caballero; Jacky G. Goetz

Cell migration is an important phenomenon involved in several developmental, physiological and pathological processes, including embryonic development, tissue homeostasis, regeneration, or tumor me...


Analytical Chemistry | 2007

Gold surface functionalization and patterning for specific immobilization of olfactory receptors carried by nanosomes.

Jasmina Vidic; Mateu Pla-Roca; Jeanne Grosclaude; Marie-Annick Persuy; Régine Monnerie; David Caballero; Abdelhamid Errachid; Yanxia Hou; Nicole Jaffrezic-Renault; Roland Salesse; Edith Pajot-Augy; J. Samitier


Nanotechnology | 2007

Electropolymerization of nano-dimensioned polypyrrole micro-ring arrays on gold substrates prepared using submerged micro-contact printing

Abdelhamid Errachid; David Caballero; Eulalia Crespo; François Bessueille; Mateu Pla-Roca; Christopher A. Mills; Francesc Teixidor; J. Samitier


Small | 2009

Direct Patterning of Anti-Human Serum Albumin Antibodies on Aldehyde-Terminated Silicon Nitride Surfaces for HSA Protein Detection†

David Caballero; J. Samitier; J. Bausells; Abdelhamid Errachid


Sensors and Actuators B-chemical | 2013

Directing polypyrrole growth by chemical micropatterns: A study of high-throughput well-ordered arrays of conductive 3D microrings

David Caballero; Laura Fumagalli; Francesc Teixidor; J. Samitier; Abdelhamid Errachid


Lab on a Chip | 2017

Tumour-vessel-on-a-chip models for drug delivery

David Caballero; Sophie M. Blackburn; Mar de Pablo; J. Samitier; Lorenzo Albertazzi


Sensor Letters | 2009

Development of Urease/Layered Double Hydroxides Nanohybrid Materials for the Urea Detection: Synthesis, Analytical and Catalytic Characterizations

Z. M. Baccar; David Caballero; Nadia Zine; Nicole Jaffrezic-Renault; Abdelhamid Errachid

Collaboration


Dive into the David Caballero's collaboration.

Top Co-Authors

Avatar

J. Samitier

University of Barcelona

View shared research outputs
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar

J. Bausells

Spanish National Research Council

View shared research outputs
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Researchain Logo
Decentralizing Knowledge