David Camerini
Harvard University
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Featured researches published by David Camerini.
Cell | 1990
David Camerini; Brian Seed
HIV infection of chimpanzees results in a chronic viremia unaccompanied by the ultimately fatal immunodeficiency that marks HIV infection in man. We show here that expression of HIV envelope proteins allows syncytium formation between cells expressing human but not chimpanzee or macaque CD4. We find that the CD4 sequences regulating cell fusion lie outside the recognized virus binding site; in the simplest exchange, chimpanzee CD4 bearing human residue 87 supports syncytium formation, while human CD4 bearing chimpanzee residue 87 does not. Neither the equilibrium nor the forward rate constants for HIV-CD4 association are affected by substitution at position 87. Infection of human cells expressing chimpanzee CD4 is insensitive to lysosomotropic agents, suggesting that viral penetration under these circumstances does not require endocytosis. The benign course of HIV infection in chimpanzees may reflect the failure of the host to support direct cell to cell transmission of the virus.
Gastroenterology | 1991
Maria Berg; Yohko Murakawa; David Camerini; Stephen P. James
The Leu-8 membrane glycoprotein is the primate homologue of the murine MEL-14 peripheral lymph node homing receptor and is expressed on a majority of circulating lymphocytes but on few lymphocytes in the intestinal lamina propria. To examine the mechanisms regulating expression of the Leu-8-molecule on lymphocytes in different tissue sites, studies of Leu-8 membrane antigen expression, Leu-8 messenger RNA, and the Leu-8 gene were performed using normal human and nonhuman primate lymphocytes. Activation of resting peripheral blood lymphocytes caused a rapid decrease in membrane Leu-8 expression, a more gradual decrease in Leu-8 messenger RNA, and an increase in expression of interleukin 2 and interleukin 2 receptor messenger RNA. However, the down regulation of Leu-8 expression during activation was reversible because both membrane Leu-8 antigen and Leu-8 messenger RNA were reexpressed after 5 days of culture. Leu-8 messenger RNA was present in lymphocytes isolated from peripheral blood, spleen, and, particularly, mesenteric lymph node, but intestinal lamina propria lymphocytes contained very low levels of Leu-8 messenger RNA. The absence of Leu-8 messenger RNA in intestinal lymphocytes and circulating Leu-8 negative lymphocytes was not caused by recent activation in vivo because these cells did not have detectable interleukin 2 messenger RNA, and intestinal lymphocytes did not express Leu-8 after culture in vitro. Phorbol myristate acetate was found to be a strong inducer of Leu-8 messenger RNA in Leu-8-positive lymphocytes; however, phorbol myristate acetate did not induce membrane Leu-8 expression or Leu-8 messenger RNA in lamina propria lymphocytes. Leu-8-negative lymphocytes in peripheral blood or lamina propria did not have evidence of deletion or rearrangement of the Leu-8 gene. Leu-8-positive Jurkat cells and peripheral blood lymphocytes and Leu-8-negative peripheral blood and intestinal lymphocytes had partial methylation of an Msp I site in proximity to the Leu-8 gene, suggesting that in Leu-8-negative lymphocytes, the Leu-8 gene previously was transcriptionally active. In summary, these studies demonstrate that intestinal lamina propria lymphocytes have the same characteristics as circulating Leu-8-negative lymphocytes with respect to their state of activation and inability to express the Leu-8 antigen. The results suggest that the majority of lymphocytes that migrate to the intestinal lamina propria are derived from the subpopulation of circulating Leu-8-negative lymphocytes.
Journal of Immunology | 1991
David Camerini; Gerd Walz; W. A. M. Loenen; J. Borst; Brian Seed
Nature | 1989
David Camerini; Stephen P. James; Ivan Stamenkovic; Brian Seed
Journal of Immunology | 1992
Toshiaki Tanaka; David Camerini; Brian Seed; Y Torimoto; Nam H. Dang; J Kameoka; H N Dahlberg; Stuart F. Schlossman; Chikao Morimoto
Archive | 1989
Janet Allen; Alejandro Aruffo; David Camerini; Leander Dr. Lauffer; Carmen Oquendo; David Simmons; Ivan Stamenkovic; Siegfried Stengelin
Archive | 1995
Brian Seed; Janet Allen; Alejandro Aruffo; David Camerini; Leander Dr. Lauffer; Carmen Oquendo; David Simmons; Ivan Stamenkovic; Siegfried Stengelin; Martine Amiot
Archive | 1990
Brian Seed; David Camerini
Archive | 1992
Brian Seed; David Camerini
Archive | 1989
Brian Dr. Seed; Janet Allen; Alejandro Aruffo; David Camerini; Carmen Oquendo; David Simmons; Ivan Stamenkovic; Leander Dr. Lauffer; Siegfried Dr. Stengelin