David J. C. Alders
VU University Amsterdam
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Featured researches published by David J. C. Alders.
Basic Research in Cardiology | 2001
A. B. Johan Groeneveld; Johannes H. G. M. van Beek; David J. C. Alders
Abstract The literature is reviewed on methods to assess heterogeneity of blood flow, substrate uptake and oxidative end energy metabolism in the normal heart, and their interrelations. Even though the factors controlling matching on the regional level remain largely obscure, the evidence that heterogeneous blood flow partially correlates to indicators of metabolism in the normal heart is accumulating, particularly in face of a correlation between acetate metabolism indicative of regional O2 consumption to microsphere blood flow. Moreover, the partial matching cannot be explained by vascular anatomical differences from one region to the other, since, although fractal theory can partially describe the branching patterns of the coronaries, vasodilation is similar among regions upon metabolic stimulation of the heart. It is dissimilar among regions, so that blood flow is redistributed, upon maximum vasodilation with adenosine or hypoxia, denoting regionally different maximum vessel diameter and flow reserve. However, regionally differing tissue composition could also contribute somewhat to regional differences in (the need for) blood flow. It is still unknown, because of technical limitations, how the foregoing measures relate to regional work load.
American Journal of Physiology-heart and Circulatory Physiology | 2011
David J. C. Alders; A. B. Johan Groeneveld; Thomas W. Binsl; Frans J. J. de Kanter; Johannes H. G. M. van Beek
Heterogeneity of regional coronary blood flow is caused in part by heterogeneity in O(2) demand in the normal heart. We investigated whether myocardial O(2) supply/demand mismatching is associated with the myocardial depression of sepsis. Regional blood flow (microspheres) and O(2) uptake ([(13)C]acetate infusion and analysis of resultant NMR spectra) were measured in about nine contiguous tissue samples from the left ventricle (LV) in each heart. Endotoxemic pigs (n = 9) showed hypotension at unchanged cardiac output with a fall in LV stroke work and first derivative of LV pressure relative to controls (n = 4). Global coronary blood flow and O(2) delivery were maintained. Lactate accumulated in arterial blood, but net lactate extraction across the coronary bed was unchanged during endotoxemia. When LV O(2) uptake based on blood gas versus NMR data were compared, the correlation was 0.73 (P = 0.007). While stable over time in controls, regional blood flows were strongly redistributed during endotoxin shock, with overall flow heterogeneity unchanged. A stronger redistribution of blood flow with endotoxin was associated with a larger fall in LV function parameters. Moreover, the correlation of regional O(2) delivery to uptake fell from r = 0.73 (P < 0.001) in control to r = 0.18 (P = 0.25, P = 0.009 vs. control) in endotoxemic hearts. The results suggest a redistribution of LV regional coronary blood flow during endotoxin shock in pigs, with regional O(2) delivery mismatched to O(2) demand. Mismatching may underlie, at least in part, the myocardial depression of sepsis.
BMC Systems Biology | 2013
Hannes Hettling; David J. C. Alders; Jaap Heringa; Thomas W. Binsl; A. B. Johan Groeneveld; Johannes H. G. M. van Beek
BackgroundThe aerobic energy metabolism of cardiac muscle cells is of major importance for the contractile function of the heart. Because energy metabolism is very heterogeneously distributed in heart tissue, especially during coronary disease, a method to quantify metabolic fluxes in small tissue samples is desirable. Taking tissue biopsies after infusion of substrates labeled with stable carbon isotopes makes this possible in animal experiments. However, the appreciable noise level in NMR spectra of extracted tissue samples makes computational estimation of metabolic fluxes challenging and a good method to define confidence regions was not yet available.ResultsHere we present a computational analysis method for nuclear magnetic resonance (NMR) measurements of tricarboxylic acid (TCA) cycle metabolites. The method was validated using measurements on extracts of single tissue biopsies taken from porcine heart in vivo. Isotopic enrichment of glutamate was measured by NMR spectroscopy in tissue samples taken at a single time point after the timed infusion of 13C labeled substrates for the TCA cycle. The NMR intensities for glutamate were analyzed with a computational model describing carbon transitions in the TCA cycle and carbon exchange with amino acids. The model dynamics depended on five flux parameters, which were optimized to fit the NMR measurements. To determine confidence regions for the estimated fluxes, we used the Metropolis-Hastings algorithm for Markov chain Monte Carlo (MCMC) sampling to generate extensive ensembles of feasible flux combinations that describe the data within measurement precision limits. To validate our method, we compared myocardial oxygen consumption calculated from the TCA cycle flux with in vivo blood gas measurements for 38 hearts under several experimental conditions, e.g. during coronary artery narrowing.ConclusionsDespite the appreciable NMR noise level, the oxygen consumption in the tissue samples, estimated from the NMR spectra, correlates with blood-gas oxygen uptake measurements for the whole heart. The MCMC method provides confidence regions for the estimated metabolic fluxes in single cardiac biopsies, taking the quantified measurement noise level and the nonlinear dependencies between parameters fully into account.
American Journal of Physiology-heart and Circulatory Physiology | 2015
David J. C. Alders; A. B. Johan Groeneveld; Thomas W. Binsl; Johannes H. G. M. van Beek
In normal hearts, myocardial perfusion is fairly well matched to regional metabolic demand, although both are distributed heterogeneously. Nonuniform regional metabolic vulnerability during coronary stenosis would help to explain nonuniform necrosis during myocardial infarction. In the present study, we investigated whether metabolism-perfusion correlation diminishes during coronary stenosis, indicating increasing mismatch of regional oxygen supply to demand. Thirty anesthetized male pigs were studied: controls without coronary stenosis (n = 11); group I, left anterior descending (LAD) coronary stenosis leading to coronary perfusion pressure reduction to 70 mmHg (n = 6); group II, stenosis with perfusion pressure of about 35 mmHg (n = 6); and group III, stenosis with perfusion pressure of 45 mmHg combined with adenosine infusion (n = 7). [2-(13)C]- and [1,2-(13)C]acetate infusion was used to calculate regional O2 consumption from glutamate NMR spectra measured for multiple tissue samples of about 100 mg dry mass in the LAD region. Blood flow was measured with microspheres in the same regions. In control hearts without stenosis, regional oxygen extraction did not correlate with basal blood flow. Average myocardial O2 delivery and consumption decreased during coronary stenosis, but vasodilation with adenosine counteracted this. Regional oxygen extraction was on average decreased during stenosis, suggesting adaptation of metabolism to lower oxygen supply after half an hour of ischemia. Whereas regional O2 delivery correlated with O2 consumption in controls, this relation was progressively lost with graded coronary hypotension but partially reestablished by adenosine infusion. Therefore, coronary stenosis leads to heterogeneous metabolic stress indicated by decreasing regional O2 supply to demand matching in myocardium during partial coronary obstruction.
Archive | 1999
Johannes H. G. M. van Beek; Harald G. J. van Mil; David J. C. Alders; A. B. Johan Groeneveld; Antonie A. van Lambalgen; Frans J. J. de Kanter; Glenn J. Harrison; Joli Bussemaker
Perfusion of the heart muscle is very heterogeneous, even in the healthy heart (Bassingthwaighte et al., 1989). The most important aspect is not the heterogeneity of perfusion or metabolism per se, but whether the blood delivers the oxygen necessary for metabolism. Whether perfusion is matched or not matched to aerobic metabolism is the question. Of course, a simple answer is that one must expect a good match in the healthy heart. Indeed, in healthy volunteers no myocardial lactate production is hidden under the net lactate extraction of the heart, not even during strenuous exercise (Kaijser and Berglund, 1992). However, it is predictable that mismatch will develop between perfusion and metabolism during ischemic disease and perhaps in other stressful situations such as cardiac hypertrophy.
American Journal of Physiology-heart and Circulatory Physiology | 1999
J. H. G. M. Van Beek; H.G.J. van Mil; Richard B. King; F.J.J. de Kanter; David J. C. Alders; J. Bussemaker
Bioinformatics | 2010
Thomas W. Binsl; David J. C. Alders; Jaap Heringa; A. B. Johan Groeneveld; Johannes H. G. M. van Beek
Advances in Experimental Medicine and Biology | 1999
J. H. G. M. Van Beek; H.G.J. van Mil; David J. C. Alders; A.B.J. (Johan) Groeneveld; A.A. van Lambalgen; F.J.J. de Kanter; Glenn Harrison; J. Bussemaker
Experimental Physiology | 2007
David J. C. Alders; Richard Cornelussen; Frits W. Prinzen; Patricia A. C. Specht; Mark I. M. Noble; Angela J. Drake-Holland; Frans J. J. de Kanter; J. H. G. M. Van Beek
Mitochondrion | 2010
Johannes H. G. M. van Beek; David J. C. Alders; Hannes Hettling; Thomas W. Binsl