David L. Lentz

Early Agriculture In The Maya Lowlands

Wetland research in northern Belize provides the earliest evidence for development of agriculture in the Maya Lowlands. Pollen data confirm the introduction of maize and manioc before 3000 B.C. Dramatic deforestation, beginning ca. 2500 B.C. and intensifying in wetland environments ca. 1500-1300 B.C., marks an expansion of agriculture, which occurred in the context of a mixed foraging economy. By 1000 B.C. a rise in groundwater levels led farmers to construct drainage ditches coeval with the emergence of Maya complex society ca. 1000-400 B.C. Field manipulations often involved minor modifications of natural hummocks. Canal systems are not as extensive in northern Belize as previously reported, nor is there evidence of artificially raised planting platforms. By the Classic period, wetland fields were flooded and mostly abandoned.

Origin of extant domesticated sunflowers in eastern North America

Eastern North America is one of at least six regions of the world where agriculture is thought to have arisen wholly independently. The primary evidence for this hypothesis derives from morphological changes in the archaeobotanical record of three important crops—squash, goosefoot and sunflower—as well as an extinct minor cultigen, sumpweed. However, the geographical origins of two of the three primary domesticates—squash and goosefoot—are now debated, and until recently sunflower (Helianthus annuus L.) has been considered the only undisputed eastern North American domesticate. The discovery of 4,000-year-old domesticated sunflower remains from San Andrés, Tabasco, implies an earlier and possibly independent origin of domestication in Mexico and has stimulated a re-examination of the geographical origin of domesticated sunflower. Here we describe the genetic relationships and pattern of genetic drift between extant domesticated strains and wild populations collected from throughout the USA and Mexico. We show that extant domesticates arose in eastern North America, with a substantial genetic bottleneck occurring during domestication.

Maya Diets of the Rich and Poor: Paleoethnobotanical Evidence from Copan

Analysis of plant remains recovered from excavations at Copan in western Honduras has provided substantive data regarding agroeconomic systems of the prehistoric inhabitants. The time span of the deposits ranges from the Gordon/Uir phase (900-400 B. C.), which may have been non-Maya, to the Coner phase (A. D. 700-900+), which encompasses the collapse of the Classic Maya cultural manifestation in the valley. Several traditionally recognized mesoamerican cultigens were identified including corn, beans, and several species of Cucurbitaceae. In addition, remains of a number of economic tree species were discovered, suggesting a reliance on arboriculture as part of the subsistence strategy. Pine charcoal predominated in all deposits and may have been the preferred wood for fuel and construction. Analysis of edible-plant-species distributions from low- and high-status Late Classic dwellings using the Shannon-Weaver index revealed that elite individuals had a higher diversity of available foods, a situation that may have led to nutritional stress among lower-status individuals and, ultimately, social unrest.

Antimicrobial properties of Honduran medicinal plants.

Ninety-two plants used in the traditional pharmacopoeia of the Pech and neighboring Mestizo peoples of central Honduras are reported. The results of in vitro antimicrobial screens showed that 19 of the extracts from medicinal plants revealed signs of antifungal activity while 22 demonstrated a measurable inhibitory effect on one or more bacterial cultures. Bioassay-guided fractionation of extracts from Mikania micrantha, Neurolaena lobata and Piper aduncum produced weak to moderately active isolates. The broad spectrum of activity of the extracts helps to explain the widespread use of these plants for wound healing and other applications.

Water and sustainable land use at the ancient tropical city of Tikal, Guatemala

The access to water and the engineered landscapes accommodating its collection and allocation are pivotal issues for assessing sustainability. Recent mapping, sediment coring, and formal excavation at Tikal, Guatemala, have markedly expanded our understanding of ancient Maya water and land use. Among the landscape and engineering feats identified are the largest ancient dam identified in the Maya area of Central America; the posited manner by which reservoir waters were released; construction of a cofferdam for dredging the largest reservoir at Tikal; the presence of ancient springs linked to the initial colonization of Tikal; the use of sand filtration to cleanse water entering reservoirs; a switching station that facilitated seasonal filling and release; and the deepest rock-cut canal segment in the Maya Lowlands. These engineering achievements were integrated into a system that sustained the urban complex through deep time, and they have implications for sustainable construction and use of water management systems in tropical forest settings worldwide.

Sunflower domestication alleles support single domestication center in eastern North America

Phylogenetic analyses of genes with demonstrated involvement in evolutionary transitions can be an important means of resolving conflicting hypotheses about evolutionary history or process. In sunflower, two genes have previously been shown to have experienced selective sweeps during its early domestication. In the present study, we identified a third candidate early domestication gene and conducted haplotype analyses of all three genes to address a recent, controversial hypothesis about the origin of cultivated sunflower. Although the scientific consensus had long been that sunflower was domesticated once in eastern North America, the discovery of pre-Columbian sunflower remains at archaeological sites in Mexico led to the proposal of a second domestication center in southern Mexico. Previous molecular studies with neutral markers were consistent with the former hypothesis. However, only two indigenous Mexican cultivars were included in these studies, and their provenance and genetic purity have been questioned. Therefore, we sequenced regions of the three candidate domestication genes containing SNPs diagnostic for domestication from large, newly collected samples of Mexican sunflower landraces and Mexican wild populations from a broad geographic range. The new germplasm also was genotyped for 12 microsatellite loci. Our evidence from multiple evolutionarily important loci and from neutral markers supports a single domestication event for extant cultivated sunflower in eastern North America.

Prehistoric sunflower (Helianthus Annuus L.) domestication in Mexico

Early remains of Helianthus annuus L. unearthed at the San Andrés site in the Gulf Coast region of Tabasco, Mexico, constitute the earliest record of domesticated sunflower. Accelerator mass spectrometry (AMS) age determinations of a large domesticated seed and achene produced dates of 4130 ± 40 years before the present (B.P.) and 4085 ± 50 B.P., respectively. These discoveries challenge the longstanding hypothesis that sunflower was domesticated in eastern North America. Moreover, when considered with other recent discoveries on plant domestication, these data suggest a reconsideration of the idea that the eastern United States was an independent hearth for domestication.ResumenLos registros más antiguos del girasol lo constituyen los primeror restos deHelianthus annuus L. desenter-rados en el sitio de San Andrés, Tabasco, en la costa del Golfo de México. Mediante el acelerador de espectrometría de masas se determinó la edad de una semilla grande domesticada y un aquenio se obtuvieron dos fechas de 4130 ± 40 años antes del presente (A.P.) y 4085 ± 50 A.P., respectivamente. Estos descubrimientos ponen en duda la hipótesis de hace tiempo que dice que el girasol fue domesticado en el este de None América. También, cuando se tomaron en cuenta otros descubrimientos sobre domesticatión de plantas, estos datos suguieren una reconsideratión de la idea que el este de los Estados Unidos fue un centro independiente de domesticatión.

Sunflower (Helianthus annuus L.) as a pre-Columbian domesticate in Mexico.

Mexico has long been recognized as one of the worlds cradles of domestication with evidence for squash (Cucurbita pepo) cultivation appearing as early as 8,000 cal B.C. followed by many other plants, such as maize (Zea mays), peppers (Capsicum annuum), common beans (Phaseolus vulgaris), and cotton (Gossypium hirsutum). We present archaeological, linguistic, ethnographic, and ethnohistoric data demonstrating that sunflower (Helianthus annuus) had entered the repertoire of Mexican domesticates by ca. 2600 cal B.C., that its cultivation was widespread in Mexico and extended as far south as El Salvador by the first millennium B.C., that it was well known to the Aztecs, and that it is still in use by traditional Mesoamerican cultures today. The sunflowers association with indigenous solar religion and warfare in Mexico may have led to its suppression after the Spanish Conquest. The discovery of ancient sunflower in Mexico refines our knowledge of domesticated Mesoamerican plants and adds complexity to our understanding of cultural evolution.

Recovery from disuse osteopenia coincident to restoration of muscle strength in mdx mice

The objective of this study was to compare the tibial structure and the strength of the tibia during muscle weakness and after recovery in mdx mice (which demonstrate X-linked muscular dystrophy and subsequent muscle regeneration) and age-matched control mice. The extent of disuse atrophy produced by muscle weakness and recovery following restoration of normal muscle strength could then be determined. The tibiae adjacent to weakened tibialis anterior muscles of 4-week-old mdx mice had significantly reduced radiographic density (p < 0.05) and cortical thickness (p < 0.001), and increased porosity (p < 0.001) compared to age-matched controls, suggesting development of disuse osteopenia. Significantly less force was required to break mdx tibiae than age-matched control tibiae (p < 0.05). In addition, Sharpeys fiber density was reduced (p < 0.001), suggesting a weakened attachment of the tibialis anterior muscle to bone. At 12 weeks, during the period of muscle regeneration, mdx tibial cortical thickness (p < 0.001) and porosity (p < 0.01) remained significantly lower, but percent calcium and Sharpeys fiber and radiographic densities were significantly greater (p < 0.001) than in age-matched controls, suggesting that bone mineralization and muscle attachment strength had increased to above normal levels in parallel with recovery of strength by the attached muscle. By 18 weeks, mdx tibial cross-sectional area, cortical thickness, and porosity remained significantly less (p < 0.001) than normal. Although Sharpeys fiber density was greater than in age-matched controls (p < 0.001) by 18 weeks, mdx tibial percent calcium (p < 0.005) and Sharpeys fiber density (p < 0.001) were significantly reduced from levels in 12-week-old mdx animals. There was significantly less deformation of the tibia prior to fracture in mdx than control tibiae at 18 weeks of age, suggesting tibial brittleness. Thus, at the site of attachment of mdx muscle to osteopenic bone, the remodelling which accompanies recovery of muscle strength is atypical, and produces an attachment of greater strength than function appears to require. These observations suggest that data are needed regarding bone mass and muscle-bone attachments in humans with disuse osteopenia, DMD, and other neuromuscular diseases.

Antimicrobial Properties of the Constituents of Piper aduncum

An ethanol extract of the leaves of Piper aduncum demonstrated good antimicrobial activity. Bioassay‐directed fractionation of this extract led to the isolation of 4‐methoxy‐3,5‐bis(3′‐methylbenzoic acid) 1, 2,6‐dihydroxy‐4‐methoxy‐chalcone 2, nervogenic acid 3 and 2,2‐dimethoxy‐8‐(3‐methyl‐2‐butenyl)‐2H‐chromene‐6‐carboxylic acid 4. These compounds showed considerable in vitro antifungal and antibacterial activity.