David Montes González

Melatonin Counteracts Alterations in Oxidative Metabolism and Cell Viability Induced by Intracellular Calcium Overload in Human Leucocytes: Changes with Age

Ageing is associated with an increased production of free radicals and alterations in the mechanisms of adaptation to oxidative stress. In fact, the free radical theory of ageing proposes that deleterious actions of free radicals are responsible for the functional deterioration associated with ageing. Moreover, a close relationship exists between calcium homeostasis and oxidative stress. The current work was aimed at proving that intracellular calcium overload induced by N-formyl-methionyl-leucyl-phenylalanine (FMLP) and/or thapsigargin leads to oxidative stress. We additionally examined the effect of melatonin on the levels of reactive oxygen species (ROS) and cell viability in human leucocytes collected from young (20-30-year-old) and elderly (65-75-year-old) individuals under both basal and oxidative stress-induced conditions. Treatments with 10 nM FMLP and/or 1 microM thapsigargin induced a transient increase in cytosolic free-calcium concentration ([Ca(2 + )](c)) in human leucocytes due to calcium release from internal stores, and led in turn to oxidative stress, as assessed by intracellular ROS measurement. Non-treated leucocytes from aged individuals exhibited higher ROS levels and lower rates of cell survival when compared to leucocytes from young individuals. Similar results were obtained in FMLP and/or thapsigargin-treated leucocytes from elderly individuals when compared to those from the young individuals. Melatonin treatment significantly reduced both hydrogen peroxide (H(2)O(2)) and superoxide anion levels, likely due to its free-radical scavenging properties, and enhanced leucocyte viability in both age groups. Therefore, melatonin may be a useful tool for the treatment of disease states and processes where an excessive production of oxidative damage occurs.

A review of the measurement procedure of the ISO 1996 standard. Relationship with the European Noise Directive

• ISO 1996-2 standard and the accuracy of estimations of noise doses were

Noise Pollution and Urban Planning

Noise pollution distribution in each city around the world is necessarily influenced by its own design. A lot of factor associated to urban planning have a considerable effect on volume of traffic, vehicles distribution, traffic conditions, etc. And it is know that, from a temporal and spatial point of view, the most important source of noise in cities is road traffic. For that, good relationships between urban planning and different factors such as urban density, urban morphology, urban land use, street distribution, street environment and green spaces are being founded. In this way, the fact of finding a sustainable city could be closer, at least with respect to noise pollution. A good knowledge of these relationships would allow better prediction, analysis and prevention of such pollution through an effective design of urban environments. However, although in the first decade of XXI century these relationships were treated in some works, only some aspects of these problems were considered, essentially focused on street functionality. In the last years, this topic has reached more important development and more studies focused on the analysis of the relationships between the distributions of pollution and urbanism. This work makes a revision of spatial sampling methodologies for noise pollution assessment in relation with urban planning and a review of studies that have analysed the relationships between urban noise and different specifics aspects of urban design.