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Dive into the research topics where David P. Gearing is active.

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Featured researches published by David P. Gearing.


Nature | 1997

Neurotactin, a membrane-anchored chemokine upregulated in brain inflammation

Yang Pan; Clare Lloyd; Hong Zhou; Sylvia Dolich; Jim Deeds; Jose-Angel Gonzalo; Jim Vath; Mike Gosselin; Jingya Ma; Barry J. Dussault; Elizabeth A. Woolf; Geoff Alperin; Janice A. Culpepper; Jose Carlos Gutierrez-Ramos; David P. Gearing

Chemokines are small secreted proteins that stimulate the directional migration of leukocytes and mediate inflammation. During screening of a murine choroid plexus complementary DNA library, we identified a new chemokine, designated neurotactin. Unlike other chemokines, neurotactin has a unique cysteine pattern, Cys-X-X-X-Cys, and is predicted to be a type 1 membrane protein. Full-length recombinant neurotactin is localized on the surface of transfected 293 cells. Recombinant neurotactin containing the chemokine domain is chemotactic for neutrophils both in vitro and in vivo. Neurotactin messenger RNA is predominantly expressed in normal murine brain and its protein expression in activated brain microglia is upregulated in mice with experimental autoimmune encephalomyelitis, as well as in mice treated with lipopolysaccharide. Distinct from all other chemokine genes, the neurotactin gene is localized to human chromosome 16q. Consequently we propose that neurotactin represents a new δ-chemokine family and that it may play a role in brain inflammation processes.


Nature | 2001

Twisted gastrulation can function as a BMP antagonist

Chenbei Chang; Douglas A. Holtzman; Samantha Chau; Troy Chickering; Elizabeth A. Woolf; Lisa M. Holmgren; Jana Bodorova; David P. Gearing; William E. Holmes; Ali H. Brivanlou

Bone morphogenetic proteins (BMPs), including the fly homologue Decapentaplegic (DPP), are important regulators of early vertebrate and invertebrate dorsal–ventral development. An evolutionarily conserved BMP regulatory mechanism operates from fly to fish, frog and mouse to control the dorsal–ventral axis determination. Several secreted factors, including the BMP antagonist chordin/Short gastrulation (SOG), modulate the activity of BMPs. In Drosophila, Twisted gastrulation (TSG) is also involved in dorsal–ventral patterning, yet the mechanism of its function is unclear. Here we report the characterization of the vertebrate Tsg homologues. We show that Tsg can block BMP function in Xenopus embryonic explants and inhibits several ventral markers in whole-frog embryos. Tsg binds directly to BMPs and forms a ternary complex with chordin and BMPs. Coexpression of Tsg with chordin leads to a more efficient inhibition of the BMP activity in ectodermal explants. Unlike other known BMP antagonists, however, Tsg also reduces several anterior markers at late developmental stages. Our data suggest that Tsg can function as a BMP inhibitor in Xenopus; furthermore, Tsg may have additional functions during frog embryogenesis.


Molecular and Cellular Biology | 1997

Characterization of a neuregulin-related gene, Don-1, that is highly expressed in restricted regions of the cerebellum and hippocampus.

Samantha J. Busfield; Donna A. Michnick; Troy Chickering; Tracy L. Revett; Jingya Ma; Elizabeth A. Woolf; Christopher A. Comrack; Barry J. Dussault; Jessica Woolf; Andrew D. J. Goodearl; David P. Gearing

Members of the epidermal growth factor family of receptors have long been implicated in the pathogenesis of various tumors, and more recently, apparent roles in the developing heart and nervous system have been described. Numerous ligands that activate these receptors have been isolated. We report here on the cloning and initial characterization of a second ligand for the erbB family of receptors. This factor, which we have termed Don-1 (divergent of neuregulin 1), has structural similarity with the neuregulins. We have isolated four splice variants, two each from human and mouse, and have shown that they are capable of inducing tyrosine phosphorylation of erbB3, erbB4, and erbB2. In contrast to those of neuregulin, high levels of expression of Don-1 are restricted to the cerebellum and dentate gyrus in the adult brain and to fetal tissues.


Nature | 1988

Myeloid leukaemia inhibitory factor maintains the developmental potential of embryonic stem cells.

R.Lindsay Williams; Douglas J. Hilton; Shirley Pease; Tracy A. Willson; Colin L. Stewart; David P. Gearing; Erwin F. Wagner; Donald Metcalf; Nicos A. Nicola; Nicholas M. Gough


Genomics | 2000

Identification and gene organization of three novel members of the IL-1 family on human chromosome 2.

S.J. Busfield; C.A. Comrack; G. Yu; Troy Chickering; John S. Smutko; H. Zhou; K.R. Leiby; Lisa M. Holmgren; David P. Gearing; Y. Pan


Archive | 1998

Novel human delta3 compositions and therapeutic and diagnostic uses therefor

Sean A. Mccarthy; David P. Gearing


Nature | 1988

Myeloid leukaemia inhibitory factor maintains the develop-mental potential of embryonic stem cells

R. C. Williams; Dj Hilton; Shirley Pease; Ta Willson; Cl Stewart; David P. Gearing; Ef Wagner; Donald Metcalf; Nicos Anthony Nicola; Nicholas M. Gough


Archive | 2007

Egf-like nucleic acids and polypeptides and uses thereof

Samantha J. Busfield; David P. Gearing


Archive | 2003

EGF-like nucleic acids

Samantha J. Busfield; David P. Gearing


Archive | 2002

SECRETED NEURAL ADHESION PROTEINS

Sean A. Mccarthy; David P. Gearing; Douglas Adam Levinson

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Sean A. Mccarthy

Millennium Pharmaceuticals

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Troy Chickering

Millennium Pharmaceuticals

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Barry J. Dussault

Millennium Pharmaceuticals

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Jingya Ma

Millennium Pharmaceuticals

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Lisa M. Holmgren

Millennium Pharmaceuticals

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Shirley Pease

Ludwig Institute for Cancer Research

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Donald Metcalf

Walter and Eliza Hall Institute of Medical Research

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