David Rifkind

Coccidioidouria: Clinical Significance

Abstract Twelve patients had urine cultures positive forCoccidioides immitis. Ten patients showed the usual criteria for dissemination, but 2 were believed, before urine culture, to have only chron...

TRANSFER OF DELAYED HYPERSENSITIVITY TO MICE WITH HUMAN IMMUNE CELL EXTRACTS

Publisher Summary This chapter describes the transfer of delayed hypersensitivity to mice with human immune cell extracts. Transfer factor was prepared from the buffy coat layer of peripheral human leucocytes by the method of Lawrence. Donors were tested with commercial coccidioidin skin test reagent. Diameter of induration was measured for 48 h. Lymphocyte blastogenesis was carried out using the method of Mans and Novelli. The lymphocyte transforming antigens used were sonicated mycelial phase C. immitis and the skin test coccidioidin. Transfer factor was given intraperitoneally to CF#1 mice and an inbred strain. The mice were used at age 10–14 weeks. Forty eight hours after transfer factor was administered the mice were tested for sensitivity to these antigens. Measurements were made just prior to antigen injection, as control and at 2–4, 24 and 48 h later. The response was taken at the specified times after antigen injection. The data suggested that transfer factor from skin test and transformation positive human donors transfers delayed hypersensitivity to mice. In contrast, transfer factor from negative normal donors did not.