David Velázquez

High Diversity of the Viral Community from an Antarctic Lake

Shivering Viromes Despite its icy reputation, freshwater ponds and lakes do occur in Antarctica, and open freshwater can be found for a few brief weeks during the austral summer. The ecology of these lakes is, as expected, rather specialized to cope with the extreme seasonal conditions. In a metagenomic study, López-Bueno et al. (p. 858) inspected the virus community of Lake Limnopolar on Livingston Island and found an unexpectedly rich genetic diversity. A dominant group of previously unidentified single-stranded DNA viruses was found, and a striking shift after ice-melt in spring from single-stranded to double-stranded DNA viruses was observed, probably as their algal hosts started to bloom with increasing daylight hours. The diverse viruses may donate specialized genes that host organisms can also exploit to aid their survival under winter extremes of heat and light deprivation. Virus populations in polar freshwater lakes show marked shifts in composition during ice melt. Viruses are the most abundant biological entities and can control microbial communities, but their identity in terrestrial and freshwater Antarctic ecosystems is unknown. The genetic structure of an Antarctic lake viral community revealed unexpected genetic richness distributed across the highest number of viral families that have been found to date in aquatic viral metagenomes. In contrast to other known aquatic viromes, which are dominated by bacteriophage sequences, this Antarctic virus assemblage had a large proportion of sequences related to eukaryotic viruses, including phycodnaviruses and single-stranded DNA (ssDNA) viruses not previously identified in aquatic environments. We also observed that the transition from an ice-covered lake in spring to an open-water lake in summer led to a change from a ssDNA– to a double-stranded DNA–virus-dominated assemblage, possibly reflecting a seasonal shift in host organisms.

Regional weather survey on Byers Peninsula, Livingston Island, South Shetland Islands, Antarctica

Abstract In 2001 the LIMNOPOLAR Project was launched with the aim of addressing the suitability of freshwater ecosystems as useful sentinels of climate change. In this project, an automatic weather station was deployed on Byers Peninsula (Livingston Island, South Shetland Islands) near several freshwater ecosystems under research. Here the multi-year data recorded are presented and compared with meteorological time series from the observatories at the Spanish Juan Carlos I Station, Deception Island and Bellingshausen Station. Lake freezing and thawing periods and snow cover are also investigated. The main results indicate that Byers Peninsula is affected by the very cloudy and wet Antarctic maritime climate. Mean annual temperature is -2.8°C and summer mean temperatures are above freezing. The region shows moderate winds over the year and with moderate, mostly liquid precipitation during the summer. There is a significant linear relationship with meteorological records obtained from Juan Carlos I Station located on the east of Livingston Island. Correlations between meteorological data from both sites are high but with colder and much windier conditions on Byers Peninsula. Therefore, the usefulness and accuracy of meteorological records in the interpretation of ecosystem dynamics are presented.

Phylogeography of cylindrospermopsin and paralytic shellfish toxin-producing Nostocales cyanobacteria from Mediterranean Europe (Spain)

ABSTRACT Planktonic Nostocales cyanobacteria represent a challenge for microbiological research because of the wide range of cyanotoxins that they synthesize and their invasive behavior, which is presumably enhanced by global warming. To gain insight into the phylogeography of potentially toxic Nostocales from Mediterranean Europe, 31 strains of Anabaena (Anabaena crassa, A. lemmermannii, A. mendotae, and A. planctonica), Aphanizomenon (Aphanizomenon gracile, A. ovalisporum), and Cylindrospermopsis raciborskii were isolated from 14 freshwater bodies in Spain and polyphasically analyzed for their phylogeography, cyanotoxin production, and the presence of cyanotoxin biosynthesis genes. The potent cytotoxin cylindrospermopsin (CYN) was produced by all 6 Aphanizomenon ovalisporum strains at high levels (5.7 to 9.1 μg CYN mg−1 [dry weight]) with low variation between strains (1.5 to 3.9-fold) and a marked extracellular release (19 to 41% dissolved CYN) during exponential growth. Paralytic shellfish poisoning (PSP) neurotoxins (saxitoxin, neosaxitoxin, and decarbamoylsaxitoxin) were detected in 2 Aphanizomenon gracile strains, both containing the sxtA gene. This gene was also amplified in non-PSP toxin-producing Aphanizomenon gracile and Aphanizomenon ovalisporum. Phylogenetic analyses supported the species identification and confirmed the high similarity of Spanish Anabaena and Aphanizomenon strains with other European strains. In contrast, Cylindrospermopsis raciborskii from Spain grouped together with American strains and was clearly separate from the rest of the European strains, raising questions about the current assumptions of the phylogeography and spreading routes of C. raciborskii. The present study confirms that the nostocalean genus Aphanizomenon is a major source of CYN and PSP toxins in Europe and demonstrates the presence of the sxtA gene in CYN-producing Aphanizomenon ovalisporum.

Temperature effects on carbon and nitrogen metabolism in some Maritime Antarctic freshwater phototrophic communities

Biofilms growing on ice and benthic mats are among the most conspicuous biological communities in Antarctic landscapes and harbour a high diversity of organisms. These communities are consortia that make important contributions to carbon and nitrogen input in non-marine Antarctic ecosystems. Here, we study the effect of increasing temperatures on the carbon and nitrogen metabolism of two benthic communities on Byers Peninsula (Livingston Island, Maritime Antarctica): a biofilm dominated by green algae growing on seasonal ice, and a land-based microbial mat composed mainly of cyanobacteria. Inorganic carbon photoassimilation, urea and nitrate uptake and N2-fixation (acetylene reduction activity) rates were determined in situ in parallel at five different temperatures (0, 5, 10, 15, 25°C) using thermostatic baths. The results for the cyanobacterial mat showed that photosynthesis and N2-fixation responded positively to increased temperatures, but urea and NO3− uptake rates did not show a significant variation related to temperature. This microbial mat exhibits relatively low activity at 0°C whereas at higher temperatures (up to 15°C), N2-fixation rate increased significantly. Similarly, the maximum photosynthetic activity increased in parallel with temperature and showed no saturation up to 25°C. In contrast, the ice biofilm displayed higher photosynthetic activity at 0°C than at the other temperatures assayed, and it showed elevated photoinhibition at warmer temperatures.

Ecology and biogeochemistry of cyanobacteria in soils, permafrost, aquatic and cryptic polar habitats

Polar Regions (continental Antarctica and the Arctic) are characterized by a range of extreme environmental conditions, which impose severe pressures on biological life. Polar cold-active cyanobacteria are uniquely adapted to withstand the environmental conditions of the high latitudes. These adaptations include high ultra-violet radiation and desiccation tolerance, and mechanisms to protect cells from freeze–thaw damage. As the most widely distributed photoautotrophs in these regions, cyanobacteria are likely the dominant contributors of critically essential ecosystem services, particularly carbon and nitrogen turnover in terrestrial polar habitats. These habitats include soils, permafrost, cryptic niches (including biological soil crusts, hypoliths and endoliths), ice and snow, and a range of aquatic habitats. Here we review current literature on the ecology, and the functional role played by cyanobacteria in various Arctic and Antarctic environments. We focus on the ecological importance of cyanobacterial communities in Polar Regions and assess what is known regarding the toxins they produce. We also review the responses and adaptations of cyanobacteria to extreme environments.

Ecological relationships and stoichiometry within a Maritime Antarctic watershed

Abstract During summer, ice-free areas are common in Maritime Antarctica, and vegetation and microbial communities frequently occur in the moist parts of catchments. In this paper, we present new data and evaluate the biomass, C, N, and P content of various types of vegetation, and the water catchment of an oligotrophic lake sited at Byers Peninsula, Livingston Island, South Shetland Islands. As the main results show, the total amount of C, N, and P contained in the organisms of the watershed is 144, 0.71 and 0.018 g m-2, respectively. According to element contents, 98% of the biological C from the watershed is within mosses and microbial mats structures (79.1 and 19.0% respectively). Also, 98.7% of the N is partially distributed between moss carpets, microbial mats and lichens, 55.2, 43.5, and 3.37 x 10-7% respectively. On the other hand, 90.2% of P is within moss carpets structures. Nutrient pools in the communities of Limnopolar Lake itself are a minor component of the whole catchment.

Vertical structure of bi-layered microbial mats from Byers Peninsula, Maritime Antarctica

Abstract A summer study of the vertical structure of bi-layered microbial mats was carried out on Byers Peninsula (Livingston Island, South Shetland Islands). These benthic communities had a common basic structure that consisted of two distinct layers differing in composition, morphology and colour. Our sampling focused on mats showing more layering, which thrived over moist soils and at the bottom of ponds. The photosynthetic pigments analysis performed by high-performance liquid chromatography demonstrated a major occurrence of cyanobacteria and diatoms on these mats, the former being more abundant in relative terms on the surface and composed by morphospecies grouping into orders Oscillatoriales, Nostocales and Chroococcales. The areal chlorophyll a concentrations were slightly higher in the deeper layer although not significantly. Our microscopic and chemical analyses showed that non-active biomass accumulates at the surface. Hence, the upper layers showed the sheath pigment scytonemin and higher amounts of exopolysaccharides, as a strategy to cope with environmental stress. On the other hand, the basal layer was composed of more active photosynthetic microbiota, which also revealed a more balanced stoichiometry. Here we exemplify how environmental stresses are potentially overcome by physiological mechanisms developed by microbial mats which also shape their vertical structure.

Maritime Antarctic Lakes As Sentinels Of Climate Change

Remote lakes, such as lakes from the Maritime Antarctica, can be used as sentinels of climate change, because they are mostly free of direct anthropogenic pressures, and they experience climate change as a main stressor capable of modifying the ecosystem structure and function. In this paper, the content of a lecture that has been presented at the First Conference of Lake Sustainability, which has been centred in our studies on lakes from Byers Peninsula (Maritime Antarctica), are summarized. These included physical, chemical and biological studies of these lakes and other freshwater ecosystems, which highlighted the relevance of biotic interactions for these ecosystems and its sensibility to temperature variations and to biological invasions, which is of relevance given the acute regional warming occurring during the last decades in the area, concomitant with the enhancement of dispersion of alien species linked to the increased presence of humans.

Diversity and temporal shifts of the bacterial community associated with a toxic cyanobacterial bloom: An interplay between microcystin producers and degraders

The biodegradation of microcystins (MCs) by bacteria constitutes an important process in freshwater ecosystems to prevent the accumulation of toxins. However, little is known about the diversity and the seasonal dynamics of the bacterial community composition (BCC) involved in the degradation of MCs in nature. To explore these BCC shifts, high-throughput sequencing was used to analyse the 16S rRNA, mcyE and mlrA genes during a year in a freshwater reservoir with a toxic cyanobacterial bloom episode. The analysis of the mcyE and mlrA genes from water samples revealed the coexistence of different MC-producing and MC-degrading genotypes, respectively. The patchy temporal distribution of the mlrA genotypes (from the families Sphingomonadaceae and Xanthomonadaceae) suggests their dissimilar response to environmental conditions and the influence of other factors besides the MCs that may control their presence and relative abundance. During the maximum toxic cyanobacterial biomass and cell lysis, other bacterial taxa that lack mlr genes increased their relative abundance. Among these bacteria, those with a recognized role in the degradation of xenobiotic and other complex organic compounds (e.g., orders Myxococcales, Ellin6067, Spirobacillales and Cytophagales) were the most representative and suggest their possible involvement in the removal of MCs in the environment.

Ecosystem function decays by fungal outbreaks in Antarctic microbial mats

Antarctica harbours a remarkably diverse range of freshwater bodies and terrestrial ecosystems, where microbial mats are considered the most important systems in terms of biomass and metabolic capabilities. We describe the presence of lysis plaque-like macroscopic blighted patches within the predominant microbial mats on Livingston Island (Antarctic Peninsula). Those blighting circles are associated with decay in physiological traits as well as nitrogen depletion and changes in the spatial microstructure; these alterations were likely related to disruption of the biogeochemical gradients within the microbial ecosystem caused by an unusually high fungal abundance and consequent physical alterations. This phenomenon has been evidenced at a time of unprecedented rates of local warming in the Antarctic Peninsula area, and decay of these ecosystems is potentially stimulated by warmer temperatures.