Davut Keskin

Soluble intercellular adhesion molecule 1, tumor necrosis factor-α and interleukin-6 levels after trauma

AbstractTo investigate the levels of soluble intercellular adhesion molecule 1 (sICAM-1), tumor necrosis factor α (TNF-α) and interleukin 6 (IL-6) and their correlation with each other and with severity of organ damage in patients with trauma, 35 patients with trauma and 20 healthy volunteers serving as controls were enrolled in this study. Blood samples were taken on admission (within 6 hours after injury), as well as at 24 h, and 3, 5, 7 and 14 days after trauma. The levels of sICAM-1, IL-6 and TNF-α were higher in patients with trauma than in the control group. Changes of serum IL-6, TNF-α and sICAM levels were significantly correlated with changes of blood serum GPT, CK, LDH, blood urea nitrogen and creatinine levels in patients with trauma. Significant positive correlations between sICAM-1 and TNF-α were found at 24 h, and at 3, 5, 7 and 14 days after the trauma (r = 0.59, 0.75, 0.83, 0.49, 0.68, respectively; all p < 0.001). These data show that sICAM-1, IL-6, and TNF-α increase in patients with tra...

Levels of interleukin-2 and interleukin-6 in serum and synovial fluid of patients with rheumatoid arthritis

AbstractRheumatoid arthritis (RA) is a chronic progressive autoimmune disorder, which is characterised by symmetric, erosive synovitis and sometimes with multisystem involvement. Inflammatory diseases such as RA are associated with increased production of cytokines. We aimed to investigate interleukin-2 (IL-2) and interleukin-6 (IL-6) levels in both serum and synovial fluid (SF) of patients with rheumatoid arthritis (RA), osteoarthritis (OA), and reactive arthritis (REA). In this study, we measured erythrocyte sedimentation rate (ESR), serum C-reactive protein (CRP) levels, both serum and SF IL-2 and IL-6, in 39 patients with RA, OA, and REA.Erythrocyte sedimentation rate and C-reactive protein levels were higher in patients with RA compared with OA and REA as characteristic laboratory findings of the disease. Both serum and SF IL-2 and IL-6 levels were higher in patients with RA than in patients with OA and REA.These findings show high production of IL-2 and IL-6 in RA serum and SF, indicating that activ...

Post-traumatic multiple organ dysfunction and elevated sE-selectin levels

Abstract Objective: To investigate the changes in sE-selectin levels in the circulation of patients with major trauma, and to establish whether these changes are correlated with injury severity and multiple organ dysfunction (MOD). Patients and methods: 40 traumatic patients with injury severity score (ISS) of 16 or over and 20 healthy volunteers (control group) were included in the study. Blood samples were taken from healthy volunteers once, and from traumatic patients first during the 6 hours after the trauma, and then after 1, 3, 5 and 7 days. sE-selectin level was analyzed in these samples using enzyme-linked immunosorbent assay method. Patients were divided into two different groups: those developing and those not developing MOD according to Denver score. Results: sE-selectin levels of traumatic patients did not show any changes compared with the control group during the first 6 hours. sE-selectin level showed a significant increase from the 1st day onwards and continued until the third day. The lev...