Dayane Olímpia Gomes

Antidiarrhoeic effect of Eugenia dysenterica DC (Myrtaceae) leaf essential oil

Essential oil from Eugenia dysenterica leaves was able to inhibit both the diarrhoea and enteropooling induced by castor oil; however, the distance travelled by charcoal meal in the intestine was not change. These data suggest that the antidiarrhoeic effect of the essential oil from E. dysenterica leaves is related to its ability to inhibit intestinal secretion and/or to increase intestinal absorption.

Occurrence of anti-Leptospira spp. antibodies in Rhipidomys spp. from a forest fragment of the Brazilian Cerrado

Leptospirosis is a zoonotic disease of world importance, and its transmission depends on the interaction between humans and animals. Given the necessity to investigate potential hosts of Leptospira spp., this study verified the prevalence of different serovars in the species of Rhipidomys spp., a widespread sigmodont rodent in Brazil. The studied population originates from a semi-evergreen forest located in the county of Uberlândia, in the state of Minas Gerais. The microscopic agglutination test (MAT) was performed with 14 serovars. Thirteen out of the 43 wild rodents captured showed a positive agglutination reaction, with a greater prevalence of the serovars Pyrogenes, Copenhageni, and Canicola. This study found a prevalence of 30.3% anti-Leptospira spp. antibodies; all positive animals were reactive to more than one serovar.

Oral microbiota in healthy Bothrops atrox (Serpentes: Viperidae) and in snakes with stomatitis

The purpose of this study was to isolate bacteria found in the oral cavity of healthy Bothrops atrox and in snakes with stomatitis. The area around the snake fang sheaths were swabbed and the samples were placed in Stuart transport medium, and then seeded on blood agar and XLD agar. Gram staining and catalase and mannitol tests were performed to identify Gram positive bacteria, while biochemical screening with Rugai-lysine medium was used to identify Gram negative bacteria. Proteus spp. (37.5%), Escherichia coli (25%), Citrobacter spp. (18.76%), Serratia spp. (9.37%) and Enterobacter spp. (9.37%) were isolated from healthy snakes, while Escherichia coli (26.31%), Citrobacter spp. (21.05%), Proteus spp. (15.78%), Salmonella (10.52%), and Staphylococcus spp. (26.31 %) were isolated from snakes with stomatitis. Staphylococcus spp. in healthy snakes and in animals with stomatitis differed significantly, suggesting that this microorganism is associated with cases of stomatitis in Bothrops atrox.

Intercorrência entre leucose enzoótica e tuberculose em bovinos abatidos em frigorífico

Leukosis and tuberculosis are diseases that affect cattle and entail considerable economic losses, due to theim pact on productivity, but there are few reports that they occur simultaneously. Therefore, aimed to evaluate the complications of these diseases in cattles laughtered in Sao Gotardo, MG, these animals were positive to the tuberculin and showed lesions characteristic of the disease during the stamping. One hundred and thirty three samples of blood serum, all from positive to bovine tuberculosis, were analyzed by immuno diffusion technique in agar gel (AGID), and the prevalence of cattle that were positive by AGID test was 55.64% (74/133). The results of this research showed that tuberculos is and leukosis are diseases present in the studied regions and are disseminated in this population, with significant levels, causing risk to the health of animals and people, as the zoonotic bovine tuberculosis.

Occurrence of antibodies against Leptospira spp. In free-ranging wild canids from the Brazilian savanna

The Brazilian savanna, also known as Cerrado, is one of the worlds richest and most ecologically invaluable tropical savanna regions. There are few studies in Brazil about the diseases that affect the wild canids of this biome, which may be harmful to wildlife populations and public health. The aim of this study was to evaluate the occurrence of antibodies against Leptospira spp. in three Cerrado wild canids species using the microscopic agglutination test (MAT). Serum samples were tested from 19 crab-eating foxes (Cerdocyon thous), 14 maned wolves (Chrysocyon brachyurus), and seven hoary foxes (Lycalopex vetulus), all free-ranging animals found in the municipalities of Araguari and Uberlândia, Minas Gerais State, and Cumari, Goias State, Brazil. Fourteen (35%) of these samples were seropositive. The most frequent serovars detected in the samples were Copenhageni and Hardjo, but reactions to the serovars Autumnalis, Grippotyphosa, Hebdomadis, Wolffi, and Icterohaemorrhagiae also occurred. Notwithstanding other reported results, this study is the first to report the presence of antibodies against Leptospira spp. in L. vetulus. The three species of wild canids examined may act as potential hosts for several serovars of leptospira in Brazils savanna environment.