Dazhao Zhu

Enhancement of fluorescence emission difference microscopy using conjugated vortex phase modulation: ENHANCEMENT OF FLUORESCENCE EMISSION DIFFERENCE MICROSCOPY

In this paper, we propose and demonstrate an improved fluorescence emission difference microscopy (FED) method, exploiting programmable phase modulation for image enhancement. The main novelty of the proposed approach lies in the matched size and intensity of two excitation spots. The proposed method improves the FED performance on image quality via artefact elimination. We demonstrate the feasibility of this method through theoretical studies and experimental tests. The experimental results of nanobeads and cells validate the practical performance of this method, which can enable reliable observations at superresolution in biomedical studies.

Demonstration of Multi-mode Parallel Detection Microscopy

Four resolution enhancement microscopy methods based on parallel detection are investigated containing confocal microscopy with four pinhole sizes, Fluorescence Emission Difference Microscopy (FED) based on parallel detection, Airyscan microscopy and Virtual k-vector modulation optical microscopy (Vikmon).