Deborah J. Withington

Localizable auditory warning pulses

Three important forms of information available to the listener may be identified in any auditory warning: what (semantic), where (location) and when (perceived urgency). Each form is addressed in the present design study of auditory warning pulses. Recordings were made via a dummy head, and were presented dichotically to listeners in a left/right localization task. The first experiment identified a suitable notched noise component for providing location information in the pulse stimulus. The second experiment required participants to simultaneously identify a distinct tonal signature and the location of the sound when they were presented with one of three tonal types, or one of three compound (tone plus noise) stimuli. Response accuracy and response latency for this identification and localization task were significantly better with the compound than with tone alone stimuli. Perceived urgency of compound complex tone plus noise stimuli was investigated in the third experiment. While there may be a trade-off between localization acuity and perceived urgency, the addition of noise components to the auditory warning pulse was shown to enhance the location information available to the listener. It is suggested that some auditory warning designs will benefit from the simultaneous provision of what and where forms of information in the sounds.

GABA-induced long-term potentiation in the guinea-pig superior colliculus

Although GABA (gamma-aminobutyric acid) is the major inhibitory neurotransmitter in the brain, intense activation of GABA receptors can cause excitation under certain conditions. In the superficial layers of the guinea-pig superior colliculus (SC) slice the excitatory action of GABA (< or = 3 mM) is dominant and sufficient to induce a robust and novel form of long-term potentiation, termed LTPG, of evoked field excitatory postsynaptic potentials (fEPSPs). This action of GABA could neither be mimicked by GABA-A nor -B agonists which were found to suppress synaptic transmission. Additionally, LTPG was not inhibited by the GABA-A receptor antagonist bicuculline while the GABA-C receptor antagonist imidazol-4-acetic acid prevented LTPG. Glutamatergic synaptic transmission was found to be required, as LTPG was partially use-dependent and did not emerge when glutamate receptors of the non-NMDA type were blocked during GABA application. Moreover, LTPG declined to baseline values in the presence of the NMDA antagonist D,L-2-amino-5-phosphonovaleric acid (APV). In addition, the L-type calcium channel blocker nifedipine inhibited the induction of LTPG. It is suggested that activation of excitatory GABA non-A, non-B receptors can lead to LTP in the SC, which may be of major importance for plastic events since the content of GABA and GABA receptors are particularly high in this brain area.

The effect of binocular lid suture on auditory responses in the guinea-pig superior colliculus.

Guinea-pigs were deprived of visual experience from soon after birth until the terminal auditory mapping experiment (performed between 57 and 96 days after birth). The visual deprivation was effectuated by binocular eyelid suture. Multi-unit responses were obtained from the superior colliculus (SC) in response ot free field presentation of bursts of broad band noise. In lid sutured animals a topographically ordered auditory map of azimuthal space was demonstrated in the SC. The auditory responses were, however, not as precise or tuned when compared with normal animals. Nevertheless, lid sutured animals were not as severely affected as dark reared animals in which no auditory map is evident in the SC. This study thus highlights the difference between binocular lid suture and dark rearing as methods of visual deprivation.

The Auditory Brainstem Response of Aged Guinea Pigs

The auditory brainstem response (ABR) technique was used to investigate potential dysfunctions in the auditory brainstem of the pigmented guinea pig (Cavia porcellus) associated with biological ageing. Animals aged from 58 days to 4 years 3 months were tested. ABRs were recorded at stimulation intensities from 85 dB HL to -10 dB HL. The auditory thresholds were found to undergo marked elevations in old animals, by an average of 32 dB. From the traces obtained, four positive deflection waves were reliably recorded. The latency of each of the four waves was evaluated at different stimulation intensities in guinea pigs of different ages. Although there was a trend for the latencies to increase in old age, these differences were not statistically significant. Similarly, there were no significant age-related changes in the inter-peak intervals. The latency/intensity functions of the four waves produced parallel curves. However, the curve from the old age group was shifted to the right, by an average of 35 dB, indicative of conductive hearing loss. There was no evidence of retro-cochlear hearing loss. Therefore, it appears that the threshold elevations in the old animals can be accounted for by conductive hearing loss, presumably in the middle ear. In 24% of the old animals tested, no ABR could be elicited. It would appear that these animals had suffered severe sensorineural hearing loss.

Hair cell loss in the aged guinea pig cochlea.

The various effects of ageing on the auditory system, collectively termed presbycusis, are being studied across a wide range of animal species, including humans. One contributing factor to presbycusis is thought to be losses of the sensory hair cells in the cochlea. In this study, hair cell counts were obtained from cochleas of pigmented guinea pigs (Cavia porcellus) at ages ranging from 11 days to 4 years 7 months, using scanning electron microscopy to visualize the organ of Corti. Representative samples of the basal, middle and apical turn of the cochlea were photographed for analysis. Hair cell loss was observed, even in young animals. However, the loss was greater in the aged animals, but was not distributed evenly throughout the length of the cochlea. No significant loss of hair cells was seen in the basal (high frequency) or middle turn of the cochlea of the aged animals. In the apical (low frequency) turn, there was a significant loss of hair cells in all rows of outer hair cells (up to around 20%), and was most severe in the third row. There was no loss of apical inner hair cells in the aged animals.

Response habituation in the superficial layers of the guinea-pig superior colliculus in vitro.

Response habituation (RH) has been suggested to reflect the role of the mammalian superior colliculus (SC) as a novelty detector. In the present study, we show that RH occurs in the SC slice preparation and is caused partially by the activation of inhibitory GABA receptors. No evidence for the contribution of presynaptic transmitter release was found and the block of N-methyl-D-aspartate (NMDA) receptors facilitated RH for a higher stimulation frequency. Since RH could not be abolished completely by any treatment, it appears that other mechanisms such as a general metabolic fatigue may contribute to RH. Nevertheless, RH must be taken into account when performing repeated stimulation in the SC slice preparation.

The effect of dark-rearing, strobe-rearing and acute visual cortex removal on the visual responses in the superficial superior colliculus of the guinea-pig

Extracellular multi-unit responses to visual stimuli were recorded in the cells of the superficial layers of the superior colliculus (SC) in four groups of adult guinea-pigs: a control group, a strobe-reared group, a dark-reared group and a group with the ipsilateral visual cortex removed acutely. Single unit visual responses were also recorded in a control and a dark-reared group. When guinea-pigs were either strobe or dark-reared from birth, the number of directionally selective responses in the superficial SC decreased significantly. Acute removal of the visual cortex had no affect on the number of directionally selective cells recorded in the SC. The correlation between azimuthal visual receptive field and rostrocaudal position of the recording electrode in the SC was not significantly different from the control group following strobe, dark-rearing or acute visual cortex removal. These data imply that, during early development, visual information is necessary for directional selectivity of the visual responses in the superficial SC. However, the map of visual azimuthal space is essentially unperturbed by visual restriction (in the form of dark or strobe-rearing) or acute visual cortex removal.

The effects of monocular enucleation on the representation of auditory space in the superior colliculus of the guinea-pig

Multi-unit responses, to free-field auditory stimuli, in the superior colliculus were investigated in guinea-pigs following earlier removal of one eye. Enucleation resulted in disruption of the normal tuning parameter values and of the topographical precision of auditory responses in the SC both ipsilateral and contralateral to the enucleated eye. These data demonstrate that monocular enucleation prevents the normal development of the superior collicular auditory space map.

Auditory guidance in a smoke-filled tunnel.

As a test on auditory guidance, participants tried to find refuge and safety in a road tunnel in dense smoke with sound beacons over the escape exits. The beacons emitted a ‘shhussing’ noise with a 5-Hz pulse frequency. Three groups participated, 32 or 33 participants per group, the first with minimal instruction (sound not mentioned), the second with beacons instruction ‘sound beacons help you to orient’, and the third with full instruction ‘sound beacons over the escape exits’. The percentage of participants finding an escape exit was 16, 21, and 70%, respectively. While the potential of auditory guidance is amply demonstrated, there is the possibility that improvement in the recognition of the sound could improve escape exit usage further. It is recommended to include in the sound of the beacon the speech fragment ‘exit here’. This will probably eliminate the need for instruction. If, in the future, the beacons are used broadly (in buildings, on ships and planes), the recognition issue should diminish.

Age-related changes in auditory spatial properties of the guinea pig superior colliculus

The map of auditory space located in the deep layers of the guinea pig superior colliculus (SC) is a complex computational representation of the auditory azimuth surrounding the animal. The map undergoes a protracted developmental profile during the first postnatal month and remains plastic until well into adulthood. However, there are no data concerning the state of the collicular auditory space map in much older animals. Multi-unit responses to broadband noise stimuli presented around the azimuthal plane under anechoic conditions were recorded from the deep SC of guinea pigs of a variety of ages, up to 44 months. The data obtained show that the map remains stable up to the age of approximately 36 months. However, after this age, the map shows rapid deterioration and at 42 months, multi-unit responses did not show features consistent with a normal map. It appears that deficits accruing within the central auditory system with increasing age, combine to overcome the ability of the mechanisms of plasticity responsible for space map maintenance to keep pace with the changes, resulting in degraded SC spatial tuning with increasing age.