Deepu Joseph

Nutritional and Antioxidative Attributes of Green Mussel (Perna viridis L.) from the Southwestern Coast of India

ABSTRACT The present study reports the biochemical composition of Perna viridis from the southwestern coast of India. A balanced essential to nonessential amino acid ratio (0.7:1.1) along with the optimum and balanced quantities of vitamins, mineral nutrients, and low cholesterol contents characterized P. viridis. The n-3/n-6 polyunsaturated fatty acid ratio of P. viridis was found to be 3.7:5.3 and therefore may serve as an alternative to balance the higher intake of n-6 fatty acids. High oxyradical scavenging capacity (24–32%) and total phenolics (2–5 mg GAE−1) suggest the nutraceutical potential of P. viridis.

Nutritional Composition of Edible Oysters (Crassostrea madrasensis L.)from the Southwest Coast of India

ABSTRACT Nutritional composition of edible oysters (Crassostrea madrasensis) from the wild and cultured growth habitats from the southwest coast of India were evaluated over 4 years (2008–2011) during the premonsoon season. The important nutritional qualities of this species have been correlated with chlorophyll-a concentration, sea surface temperature, and phytoplankton density in their growth environments. The higher proportions of total polyunsaturated fatty acids, eicosapentaenoic, and docosahexaenoic acids in the edible oysters collected from the wild habitats were significantly correlated with chlorophyll-a concentration revealing the role of the phytoplanktons to contribute to the occurrence of these vital fatty acids. The ideal atherogenic index (AI); thrombogenicity index (TI); hypocholesterolemic/hypercholesterolemic ratio (HH); and balanced quantities of vitamins, minerals, amino acids, and low cholesterol contents qualified C. madrasensis as a potential health food.

Changes in the Quality of Refined Fish Oil in an Accelerated Storage Study

ABSTRACT The aim of this study was to prepare the refined oil derived from Sardinella longiceps by a step-wise procedure of degumming, bleaching, and deodorization. The refined sardine oil showed greater susceptibility toward oxidation than the crude oil, but the addition of ethyl acetate fractions of the red seaweeds Hypnea musciformis, Kappaphycus alvarezii, and Jania rubens with great antioxidant potential significantly increased the oxidative stability of the refined oil. The refined oil supplemented with the ethyl acetate fraction of these seaweeds indicated more noteworthy oxidative stability indices (≥ 0. 51 h) than α-tocopherol. The induction time was greater for refined oil added with H. musciformis (1.26 h) than butylated hydroxytoluene (1.04 h) and α-tocopherol (0.4 h). No traces of aromatic (δ 6.7–7.5 ppm), hydroperoxides, and aldehydes (δ 9–10 ppm) in the 1H-NMR spectra of the refined oil supplemented with seaweed extracts at the baseline and after the accelerated storage study demonstrated that the seaweed extracts were able to prevent the formation of secondary oxidation products in the unsaturated system during storage. This study demonstrated the potential of seaweeds as natural alternatives to the synthetic antioxidants to prevent the rancidity of the refined fish for use in food and pharmaceutical industries.

Seasonal and inter-annual lipid dynamics of spiny cheek grouper (Epinephelus diacanthus) in the southern coast of India

The muscle lipid, fatty acids and total cholesterol profiles of the spiny cheek grouper, Epinephelus diacanthus, collected from south-west (Arabian Sea) and south-east coasts (Bay of Bengal) of India were evaluated over four years (2008-2011) with regard to three seasons (pre-monsoon, monsoon and post-monsoon). Fatty acids were correlated with chlorophyll-a concentration and sea surface temperature. Lipid content, total polyunsaturated, eicosapentaenoic and docosahexaenoic fatty acids of the samples from the south-west coast showed positive correlation with chlorophyll-a concentration during the monsoon (r(2) = 0.93, 0.97, 0.97 and 0.99, respectively). Higher hypocholesterolaemic/hypercholesterolaemic ratio (>1.0) and low atherogenic ( 19% during post-monsoon), important in the human diet for their platelet anti-aggregating and blood pressure-reducing properties, for groupers collected from the south-west coast, with higher n-3/n-6 fatty acid ratio (>5.1) proved this species from the coast of the Arabian Sea to be a desirable item in the human diet.

Concentration and stabilization of C20–22 n-3 polyunsaturated fatty acid esters from the oil of Sardinella longiceps

Methyl esters of C20-22n-3 polyunsaturated fatty acids derived from sardine oil triglycerides were concentrated to 86% purity with greater than 30% recovery by argentated chromatography. The synergistic effect of ethyl acetate fractions of seaweeds Kappaphycus alvarezii, Hypnea musciformis and Jania rubens used in 0.1:0.2:0.2 (%, w/w) ratio in arresting oxidative degradation of the n-3 PUFA methyl ester concentrate was demonstrated during accelerated storage. The induction time (6.8h) and antioxidant activity indices (>24) were greater for n-3 PUFA concentrates supplemented with seaweed extracts than antioxidants BHT and α-tocopherol (<5h and <17, respectively). Nuclear Magnetic Resonance spectroscopy was employed to study the oxidative changes of fatty acid signals of PUFA concentrate during accelerated storage. Potential of seaweeds to improve the storage stability of C20-22n-3 fatty acid methyl esters was studied. This study has applications in development of food and pharmaceutical products.

Inter-annual and seasonal dynamics of amino acid, mineral and vitamin composition of silver belly Leiognathus splendens

Silver bellies, Leiognathus splendens were studied for their spatial (south-west and south-east coasts of India), annual (2008–2011) and seasonal (pre-monsoon, monsoon and post-monsoon) variations of protein, amino acids, vitamins and minerals. The monthly mean Sea Viewing Wide Field-of-view Sensor data for the period from January 2008 to December 2011 were taken into account to indicate the distribution of the photosynthetic pigment chlorophyll- a to test the hypothesis that surface productivity might be related to nutritional biochemistry of this species. The four year average total protein content and chlorophyll- a showed good correlation during monsoon on the south-west coast and monsoon/post-monsoon on the south-east coast, suggesting that the protein content is prejudiced by the chlorophyll- a concentration. Amino acid scores observed monsoon maxima along the south-west and south-east coasts. Significant seasonal variations in vitamin content were observed at the study locations with high content of vitamins D 3 , E, K 1 and C on the south-west coast. Na content was maximal during pre-monsoon on the south-west coast, while post-monsoon maxima of Ca and K content were observed. The Fe, Mn and Zn were abundant in the samples collected from the south-west coast. The concentration of Se exhibited maximum values post-monsoon along the south-west and south-east coasts. The present study demonstrated L. splendens as a valuable source of the protein, amino acids, minerals and vitamins, showing that this low-value species is a good source of well balanced proteins with high biological value to be qualified as a preferred healthy food for human consumption.

Production and characterization of refined oils obtained from Indian oil sardine (Sardinella longiceps).

Crude Sardinella longiceps oil was refined in different stages such as degumming, neutralization, bleaching, and deodorization. The efficiency of these processes was evaluated on the basis of free fatty acid (FFA), peroxide (PV), p-anisidine (pAV), total oxidation (TOTOX), thiobarbituric acid reactive species (TBARS) values, Lovibond CIE-L*a*b* color analyses, and (1)H NMR or GC-MS experiments. The utilities of NMR-based proton signal characteristics as new analytical tools to understand the signature peaks and relative abundance of different fatty acids and monitoring the refining process of fish oil have been demonstrated. Phosphoric acid (1%) was found to be an effective degumming reagent to obtain oil with the lowest FFA, PV, pAV, TOTOX, and TBARS values and highest color reduction. Significant reduction in the contents of hydrocarbon functionalities as shown by the decrease in proton integral in the characteristic (1)H NMR region was demonstrated by using 1% H3PO4 during the course of the degumming process. A combination (1.25:3.75%) of activated charcoal and Fullers earth at 3% concentration for a stirring time of 40 min was found to be effective in bleaching the sardine oil. This study demonstrated that unfavorable odor-causing components, particularly low molecular weight carbonyl compounds, could successfully be removed by the refining process. The alkane-dienals/alkanes, which cause unfavorable fishy odors, were successfully removed by distillation (100 °C) under vacuum with aqueous acetic acid solution (0.25 N) to obtain greater quality of refined sardine oil, a rich source of essential fatty acids and improved oxidative stability. The present study demonstrated that the four-stage refinement process of sardine oil resulted in a significant improvement in quality characteristics and nutritional values, particularly n-3 PUFAs, with improved fish oil characteristics for use in the pharmaceutical and functional food industries.

Inter Annual and Seasonal Dynamics in Lipidic Signatures of Sardinella longiceps

ABSTRACT Seasonal variations (premonsoon, monsoon, and postmonsoon) of the lipid, fatty acid, and total cholesterol profile in edible tissues of Sardinella longiceps collected from the southwest and southeast coasts of India over 4 yr (2008–2011) were studied. The correlation between the fatty acid composition with chlorophyll-a concentration, sea surface temperature, total phytoplankton density, diatom concentration, and fatty acid profiles of pooled stomach microalgal content were also evaluated. Significant correlation was observed between oil sardine and pooled stomach microalgal content polyunsaturated fatty acids along with chlorophyll-a concentration, especially during the monsoon at the southwest coast and monsoon and postmonsoon at the southeast coast of the Indian subcontinent. Similarly, the health indices such as atherogenic index, thrombogenicity index, total cholesterol, and hypocholesterolemic/hypercholesterolemic ratio of oil sardine were found to be ideal, which contributed toward its parameters to be qualified as an ideal health food.

Antioxidative Status of a Nutrient Enriched Formulation of Perna viridis in a Time Series Shelf-Life Study

ABSTRACT The freeze-dried concentrate of green mussel, Perna viridis, was added with naturally derived antioxidative oleoresins of rosemary and turmeric along with trace amounts of other natural additives to prepare different treatments and was subjected to accelerated shelf life study (50°C) to evaluate its oxidative stability. The total oxyradical scavenging activities of samples with higher concentrations of turmeric over rosemary demonstrated the prominent role of the turmeric extracts to arrest free radical chain reactions. Time dependent antioxidative status in relation to the nutritional compositions was evaluated to identify the oxidative changes for different treatments. The addition of natural additives with polyphenolic moieties in the treatments shielded the mussel’s unsaturated fatty acids against oxidation as evident by higher polyunsaturated fatty acids in the two treatments as compared to control after 90 days of accelerated shelf-life study. Higher n-3/n-6 ratios after 90 days’ storage were also observed in these treatments. A threshold level of combined turmeric (0.8 g/100 g) and rosemary (0.4 g/100 g) was sufficient for optimum antioxidant activity and to maintain nutritional composition of the green mussel extract at desired levels.

Interannual and Seasonal Dynamics in Lipidic Signatures ofTrichiurus lepturus

ABSTRACT Interannual and seasonal (premonsoon, monsoon, and postmonsoon) variability in the lipid, fatty acids, and total cholesterol content of ribbon fish, Trichiurus lepturus were determined for a period of 4 years (2008–2011) along the southwest (SW) and southeast (SE) coasts of India. The lipidic compositions were studied along with chlorophyll-a concentration and sea surface temperature (SST) data obtained from MODIS and Sea WiFS. The lipid content shows seasonal fluctuations with higher values in the postmonsoon season along the SE coast and during the monsoon in the SW coast. Among polyunsaturated fatty acids (PUFAs), docosahexaenoic acid (DHA) dominated over eicosapentaenoic acid (EPA) throughout the seasons except in winter on the SE coast. The health indices such as atherogenic/thrombogenicity indices, total cholesterol, and hypocholesterolemic/hypercholesterolemic ratio of ribbon fish were found to be ideal, which contributed toward its parameters to be qualified as a potential health food.