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Featured researches published by Delma Pegolo Alves.


Journal of Food Protection | 2012

Survival In Vitro and Virulence of Trypanosoma cruzi in Acai Pulp in Experimental Acute Chagas Disease

Rodrigo Labello Barbosa; Viviane Liotti Dias; Karen Signori Pereira; Flávio Luis Schmidt; Regina Maura Bueno Franco; Ana Maria Aparecida Guaraldo; Delma Pegolo Alves; Luiz Augusto Corrêa Passos

Chagas disease is a parasitic infection with high socioeconomic impact throughout Latin America. Although this severe, incurable disease can be transmitted by several routes, oral transmission is currently the most important route in the Amazon Basin. Açaí pulp has nutritional properties and is popular throughout Brazil and abroad. However, this pulp has been associated with microepidemics of acute Chagas disease (ACD) in northern Brazil, where açaí fruit is the main food supplement. In this study, we examined the in vitro survival and in vivo virulence of Trypanosoma cruzi Y strain in açaí pulp. Aliquots of in natura açaí pulp produced in Belém city in the northern Brazilian state of Pará were mixed with 10⁵ trypomastigotes. The samples were incubated at room temperature or at 4 or -20°C for various periods, and the parasites were isolated by forced sieving. The resulting eluates were examined by microscopy, and the trypomastigotes were administered intraperitoneally, orally, or by gavage to immunodeficient mice (C.B-17-Prkdc(scid)/PasUnib) that had been pretreated with antibiotics. Parasitemia was quantified by the Brener method, and mortality was recorded daily. All routes of administration resulted in ACD. A 5-day delay in the onset of parasitemia occurred with oral administration. The survival and virulence of the parasites were unaffected by prior incubation at room temperature for 24 h, at 4°C for 144 h, and at -20°C for 26 h. These results indicate that T. cruzi can survive and retain its virulence in açaí pulp under various conditions and that cooling and freezing are not suitable methods for preventing foodborne ACD.


Journal of Food Protection | 2016

Virulence of Trypanosoma cruzi in Açai (Euterpe oleraceae Martius) Pulp following Mild Heat Treatment

Rodrigo Labello Barbosa; Karen Signori Pereira; Viviane Liotti Dias; Flávio Luis Schmidt; Delma Pegolo Alves; Ana Maria Aparecida Guaraldo; Luiz Augusto Corrêa Passos

Outbreaks of acute Chagas disease (ACD) in northern Brazil can be caused by the ingestion of unprocessed açai pulp contaminated with Trypanosoma cruzi . The aim of this study was to determine the minimum thermal process required to inactivate T. cruzi in açai pulp. Trypomastigotes (100,000) of T. cruzi Y strain were added to 0.15 M NaCl or açai pulp and continuously mixed while being heat treated at 37 to 49°C for up to 1 h. When necessary, parasites were separated from açai pulp by forced sieving. Inocula were administrated intraperitoneally in inbred immunodeficient C.B-17-Prkdcscid/Pas Unib mice, and the recipients were monitored for parasitemia and mortality. Mice received prophylactic antibiotic therapy by using cephalexin to prevent bacterial infection from the açai pulp. T. cruzi retained its virulence in 0.15 M NaCl and açai pulp at 44 ± 0.1°C for 10 min and at 43 ± 0.1°C for 20 min, respectively, causing ACD and death in mice up to 24 days after infection. Incubation of açai pulp inoculum above 43°C for 20 min neutralized T. cruzi virulence, thereby preventing ACD and death in murine recipients. The heating of açai pulp above 43°C for 20 min is a practical and effective measure to prevent foodborne ACD caused by T. cruzi .


International Scholarly Research Notices | 2013

Infectivity of Giardia duodenalis Cysts from UV Light-Disinfected Wastewater Effluent Using a Nude BALB/c Mouse Model

Luciana Urbano dos Santos; Delma Pegolo Alves; Ana Maria Aparecida Guaraldo; Romeu Cantusio Neto; Maurício Durigan; Regina Maura Bueno Franco

Giardia duodenalis is a protozoan of public health interest that causes gastroenteritis in humans and other animals. In the city of Campinas in southeast Brazil, giardiasis is endemic, and this pathogen is detected at high concentrations in wastewater effluents, which are potential reservoirs for transmission. The Samambaia wastewater treatment plant (WWTP) in the city of Campinas employs an activated sludge system for sewage treatment and ultraviolet (UV) light for disinfection of effluents. To evaluate this disinfection process with respect to inactivating G. duodenalis cysts, two sample types were investigated: (i) effluent without UV disinfection (EFL) and (ii) effluent with UV disinfection (EFL+UV). Nude immunodeficient BALB/c mice were intragastrically inoculated with a mean dose of 14 cysts of G. duodenalis recovered from effluent from this WWTP, EFL, or EFL+UV. All animals inoculated with G. duodenalis cysts developed the infection, but animals inoculated with UV-exposed cysts released a lower average concentration of cysts in their faeces than animals inoculated with cysts that were not UV disinfected. Trophozoites were also observed in both groups of animals. These findings suggest that G. duodenalis cysts exposed to UV light were damaged but were still able to cause infection.


Epidemiologia e Serviços de Saúde | 2012

Sobrevivência e infectividade do Trypanosoma cruzi na polpa de açaí: estudo in vitro e in vivo

Luiz Augusto Corrêa Passos; Ana Maria Aparecida Guaraldo; Rodrigo Labello Barbosa; Viviane Liotti Dias; Karen Signori Pereira; Flávio Luis Schmidt; Regina Maura Bueno Franco; Delma Pegolo Alves


Archiv Der Pharmazie | 2007

Pharmacokinetic profile of atenolol aspirinate.

Ana C. Montes-Gil; Marcos Zanfolin; Cristina E. Okuyama; Sergio Lilla; Delma Pegolo Alves; Vincenzo Santagada; Elisa Perissutti; Antonio Lavecchia; Ferdinando Fiorino; Beatrice Severino; Giuseppe Caliendo; Fernanda B.M. Priviero; Gustavo D. Mendes; José L. Donato; Gilberto De Nucci


Sínteses: Revista Eletrônica do SIMTEC | 2016

Adaptação de manta térmica corporal, utilizada para monitorar aquecimento em pequenos animais submetidos à anestesia geral

Marcos Zanfolin; Renato Faro; Delma Pegolo Alves; Antonio Ferreira Filho


Sínteses: Revista Eletrônica do SIMTEC | 2016

Efeito do bay 41-2272, na pressão arterial de ratos sob o tratamento crônico com inibidor da síntese de óxido nítrico

Marcos Zanfolin; Renato Faro; E. G. Araujo; Marco Aurelio Guarado; Edson Antunes; Gilberto De Nucci; Delma Pegolo Alves


Sínteses: Revista Eletrônica do SIMTEC | 2016

Implementação de metodologia para monitorização microbiana no ambiente de produção de matrizes de camundongos e ratos livres de agentes patogênicos especificados

Edivana Aparecida Vespa Alves; Rovilsom Gilioli; Silvio Rogério Cardoso dos Santos; Marcos Zanfolin; Armando Ferreira Lima Filho; Delma Pegolo Alves


Sínteses: Revista Eletrônica do SIMTEC | 2016

Implantação de sistema de radiotelemetria no cemib para estudo e teste de novos fármacos e monitoramento de parâmetros fisiológicos em modelos animais

Marcos Zanfolin; Renato Faro; Gilberto De Nucci; Ana Maria Aparecida Guaraldo; Edivana Aparecida Vespa Alves; Delma Pegolo Alves


Sínteses: Revista Eletrônica do SIMTEC | 2016

Estabelecimento da reação da polimerase em cadeia (PCR) para a detecção de helicobacter spp em amostras de fezes de camundongos de biotérios brasileiros

Daniele Masseli Rodrigues Demolin; Lenira Aparecida Guaraldo de Andrade; Silvio Rogério Cardoso dos Santos; Rovilson Gilioli; Delma Pegolo Alves

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Marcos Zanfolin

State University of Campinas

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Rovilson Gilioli

State University of Campinas

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Gilberto De Nucci

State University of Campinas

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Renato Faro

State University of Campinas

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