Delphine Bartosik

A method for the determination of progesterone in biological specimens

Abstract A method for the analysis of progesterone has been described which combines the isolation of radiochemically pure progesterone with quantitation based on the formation of progesterone-3,20-bisdinitrophenylhydrazone. The method has been developed for the analysis of progesterone in whole blood and tissues.

Thin-layer partition chromatography of steroids using volatile stationary phases

Abstract A simple technique for using Bush-type (volatile stationary phase) chromatographic systems on thin-layer plates composed of Silica Gel GF is described. The R F values of several steroids of biological interest are presented for both aqueous methanolic and aqueous acetic acid systems. Suggestions for the modification of paper partiton chromatography systems for use with silica gel plates are also presented.

Cholesterol precursor pools of progesterone in the bovine ovary perfused in vitro.

Summary Bovine ovaries were perfused in vitro with acetate-1-14C throughout an experiment which lasted 377 minutes. Luteal ovaries and the contralateral follicular ovaries were sequentially infused with prolactin, and then with LH. At the end of the perfusion, the specific activity of luteal cholesterol-14C was slightly greater than the specific activity of either the luteal tissue progesterone-14C or the secreted progesterone-14C. The specific activity of the cholesterol-14C isolated from the residual stromal tissue and from the follicular ovaries was much lower than that observed in the corpus luteum.