Den-itsu Shiho

Automatic liquid chromatography of organic compounds : II. Adsorption chromatography of benzene derivatives on styrene-based cation-exchange resin☆

Abstract The adsorption chromatography of benzene derivatives on styrene-based cation-exchange resin has been investigated. Adsorption isotherms of benzene derivatives are discussed and the possibility of separation and quantitative treatment is considered for some cases of non-linear isothermal adsorption. The distribution coefficient and the height equivalent to a theoretical plate, important for separation and quantitative analysis, have been calculated for thirty-eight derivatives of benzene.

Automatic liquid chromatography of organic compounds : I. Microquantitative analysis of urea type compounds by automatic liquid chromatography with a thermal detection method

Abstract The separation of urea type compounds was studied by using thermal reaction energy detection automatic liquid chromatography. A strong polystyrene-based ion-exchange resin was used as adsorbent, activated in types of sulphonic acid, sodium sulphonate, and ammonium chloride. The highest thermal detection sensitivity on liquid chromatography, ± 3/10000°, full scale was tested while changing the concentration of the eluent, namely 1.0, 0.1, and 0.01 N hydrochloric acid and water. It was illustrated that the sensitivity and reproducibility of the thermal reaction energy detection method were superior to those of colorimetry in quantitative microanalysis of urea type compounds.

Automatic liquid chromatography of organic compounds : III. The relationship between the chromatographic behaviour and the chemical structure of benzene derivatives☆

Abstract The relationship between the distribution coefficients ( K D ) of benzene derivatives on the hydrogen and sodium forms of styrene-based strong cation-exchange resins, e.g. Bio-Rad AG 50W X8, and their chemical structures are discussed. A comparison of the K D values of the hydrogen and sodium forms of the resin, the effect of the size of the halogen atom on the K D values and of the additivities of the K D values with respect to the number of carbon atoms in the alkyl groups in alkyl phenyl ethers and alkylphenols and to substituents in phenol derivatives are considered.