Deng Yulin

A novel device for cell washing, lysis and efficient extraction of intracellular proteins in a continuous automatic mode

By simultaneously subjecting cells to lysis buffer and vibration of the cell suspension fluid, effective release of intracellular contents was obtained. The synergistic effect of the applied lysis buffer and vibration on cell lysis in a membrane separation device was demonstrated for suspension cell. The resulting lysate is suitable for downstream biological sample analysis and biomedical research without requiring further separation. The cell pretreatment chamber employs a membrane separation device which provided an effective area for the cell retention, washing and lysis by peristaltic pumps and solenoid valves. To control the roller rotation direction of pump, vibrations can be produced at a certain frequency on the surface of membrane in pretreatment chamber. Then the intracellular contents were pumped into the units of filtering and ultrafiltration to remove impurities (cell fragments) and separate intracellular proteins, respectively. The result exhibits the feasibility of the proposed prototype device. The working device suggests an inexpensive, easy to be fabricated and effective way for cell pretreatment and separation of intracellular proteins, which can be easily integrated with other functional components to form a multifunctional system for analysis of biological samples.

Application of Biochemical Indexes in Ergonomics Evaluation under Extreme Conditions

A new method was designed to evaluate the ergonomics under extreme hypoxic environment. In traditional methods for ergonomics evaluation, some methods are often used, such as questionnaire survey, sound or video recording etc. The most significant feature of those methods is that interactive communication with the volunteers is needed. Those methods are, however, limitedly suitable for the ergonomics evaluation under extreme environment such as hypoxia, noxious gases etc. It is harmful to the human body under extreme environment. In this experiment, some biochemical indexes were studied and applied to evaluate the ergonomics under extreme hypoxic environment. The corresponding relationship between the changes of physiological and biochemical indexes and the ergonomics was established. Then it was used to evaluate the ergonomics under extreme hypoxia condition objectively. According to biomedical standard and the request of ergonomics evaluation, preliminary ergonomics regions under hypoxic environment were divided objectively. Under hypoxia condition, from the ergonomic point of view, 20.9% O2 is the optimal ergonomics value, 15%, 10% and 8% O2 are three important thresholds and might belong in warrant, limit and critical region respectively. This way is more practical and objective for evaluating the ergonomics under extreme conditions.

Seperation and Determination of Glycyrrhizic acid and Liquiritin in Licorice using SPE-RP-HPLC

Glycyrrhic acid (GA) and liquiritin are the two classes of bioactive components in licorice with known pharmacological effects. In our study, different methods were compared to separate GA an-d liquiritin. Solid-phase extraction (SPE) method was verified to separate GA and liquiritin effectively. The method is fast, easy, reliable, and inexpensive; especially in the quality control. Moreover, we built a new method to determine glycyrrhizic acid and liquiritin simultaneously using reversed phase high performance liquid chromatography (RP-HPLC) In the HPLC analyze, gradient elution and two wavelength were used . The regression line for GA was y =15340.6x+12.81, the regression for liquiritin line was y =9269.1x+20.63, The recovery rates were 101.3% and 99.4%; RSD were 1.01% and 0.92%.

Determination and separation of forsythoside B and ph llyrin n Fors thia suspense

Forsythia suspense is a widely used Chinese traditional medicine. Forsythoside B and phillyrin are the major bioactive components in Forsythia suspense. In this study, macroporous resin method was used to separate forsythoside B and phillyrin. It indicated that compared with solvent extraction and absorption by alumina and other common separation method, macroporous resin method can separate forsythoside B and phillyrin effectively. Moreover, this study built a new method to determine forsythoside B and phillyrin simultaneously relied on Reversed phase high performance liquid chromatography (RP-HPLC). The linearity studies, Stability, Precision, and reproducibility test satisfy the determination request.