Denise Buffe

Relation entre les antigènes du liquide amniotique et du sérum foetal

Abstract Seventeen human amniotic fluids were studied with immune sera anti-liquor amnii absorbed by normal human plasma. Four precipitin lines were revealed by immunoelectrophoresis, one of which having α 1 mobility, two with α 2 mobility, one with β mobility. The first constituent, present mainly in early amniotic fluids, was identified to be α 1 fetuin, specific protein of the foetal serum. The two constituents α 2 were found specific for the late liquor amnii; they are also seen in the amniotic membranes. The β line of the amniotic fluid at term was shown to be due to contamination by haemoglobin. The absence of an antigenic relationship between the amnioproteins and the α 1 fetuin was demonstrated.

Immunoglobulin-Producing Cells in Human Pericancerous Lymph Nodes

Immunoglobulin‐producing cells and their synthesized immunoglobulins were studied by immunofluorescent techniques in 30 human pericancerous lymph nodes and 13 lymph nodes from control subjects. The production of immunoglobulins was found to be strongest in histologically active lymph nodes; however, some lymph nodes rich in follicles contained no immuno‐globulin‐producing cells. The amount of immunoglobulins produced appears to have no relation to the presence or absence of metastatic cells in the lymph nodes—the cells with immunoglobulins are not usually in contact with the cancer cells. The predominance of immunoglobulin A in pericancerous nodes is important because antibodies of this type do not fix the complement and are not likely to be cytotoxic. The immunoglobulins thus synthesized can react against cancer cells or the products of cellular metabolism. This last hypothesis seems most probable.

Immunofluorescent Studies of Human Plasma Cells in γ and β2A Myelomas

Summary Thirty-seven bone marrow aspirates of human γ and β 2A myelomas were smeared and labelled by fluorescent antibodies against γ and β 2A globulin. Never were all the observed plasma cells reactive with fluorescent antibodies, and in half of the cases, no fixation by any plasma cell was noted. Plasma cells of different types were fluorescent, most of them having a granular structure. They were found to fix fluorescent antibodies against γ globulin or β 2A globulin, but never both.

Les Antigènes du Liquide Amniotique Humain

Summary Seventeen human liquor amnii were studied with immunosera anti-amniotic fluid, absorbed by normal human plasma. Four precipiting lines were developed by immunoelectrophoresis, one of which having α 1 mobility, two with α 2 mobility, one with β mobility. The first component, present mainly in early liquor amnii, was identified to be α 1 fetuin, the protein specific of foetal serum. The two constituents α 2 were found specific for the late amniotic fluid. They have been found also in the amniotic membranes. The β line of the amniotic fluid at term has been shown to be due to hemoglobin contamination. It has been shown that there is no antigenic relationship between the amnioproteins and the α 1 fetuin.