Denise Conceição Mesquita Dantas

Analysis of leukocytes in medication-overuse headache, chronic migraine, and episodic migraine.

(Headache 2011;51:1228‐1238)

Perfil dos usuários do serviço de teleatendimento sobre drogas de abuso VIVAVOZ

INTRODUCTION: Drug abuse is a major public health problem. Telephone interventions have been used as a treatment method. This study aimed at describing the sociodemographic profile, consumption pattern and dependence on psychoactive substances of individuals seeking help in a telephone service on drugs of abuse. METHODS: Data were collected by previously trained consultants using an electronic protocol throughout the first year of the service. Instruments were applied to find the sociodemographic profile, consumption pattern and dependence of drug users. Descriptive statistics was used to estimate distribution of variables, and the data are presented as frequencies. RESULTS: Throughout the study period there were 28,257 calls, of which 7,956 were included. In total there was higher prevalence of women, students, single individuals, older than 35 years, with incomplete primary education and family income lower than five minimum wages. Men aged 18-25 years were prevalent in the sample. The most frequently used drugs were tobacco, cannabis, alcohol and cocaine. Tobacco use was similar for both genders. Males used more illicit drugs. Most drug users were dependent, and men had higher rates of addiction to tobacco and solvents. CONCLUSIONS: These results outline the profile of individuals who seek care through a telephone service, showing the importance of these services for the population and guiding preventive actions.

Assessment of changes in nicotine dependence, motivation, and symptoms of anxiety and depression among smokers in the initial process of smoking reduction or cessation: a short-term follow-up study

INTRODUCTION The first days of a quit attempt represent an important challenge to long-term abstinence, especially because of the changes that take place over this period. OBJECTIVE To examine whether smokers who have recently changed their smoking behavior show changes in the intensity of nicotine dependence, motivational stage, or symptoms of anxiety and depression relative to smokers without recent changes in smoking behavior. METHODS Smokers attending a support group for smoking cessation in Porto Alegre, southern Brazil, were invited to participate. The program consisted of four weekly sessions. Smokers answered questionnaires covering intensity of nicotine dependence, stage of motivation, and symptoms of anxiety and depression at baseline and in the fourth week. Urine was collected at both time points, tested for cotinine concentration, and used to determine the final status of smokers. RESULTS Of the 54 smokers included in the study, 20 (37%) stopped smoking or decreased tobacco use. Both smokers who stopped or reduced tobacco use and those who did not change their behavior presented a decrease in nicotine dependence scores (p = 0.001). Conversely, only the smokers who changed behavior presented an increase in scores in the maintenance stage (p < 0.001). CONCLUSION When modifying tobacco use, smokers face a difficult process, marked by several changes. A better understanding of these changes and their implications for treatment are discussed.

Thirdhand tobacco smoke: procedures to evaluate cytotoxicity in cell cultures.

Abstract The risks associated to tobacco smoking are not ceased with smoke extinction. Many toxic compounds remain in the environment after the cigarette is extinguished and accumulated in the air or on surfaces. However, little is known about the risks of this exposure. The aim of this study was to evaluate procedures to collect thirdhand smoke (THS) and prepare the samples to perform three in vitro toxicity tests. Cellulose papers and cotton wipes were used to impregnate with nicotine solution and smoke cigarette in a chamber or in smoker’s home. Samples were immersed in methanol or Dulbecco’s modified Eagle’s medium (DMEM) to expose Hep-2 cells. MTT, neutral red uptake (NRU) and trypan blue assays were performed. The concentration of nicotine in DMEM extract of THS in paper and cotton was similar to those in methanol extract (p > 0.05). Alterations in the mitochondrial and lysosomal functions were found in both paper and cotton samples; however, the cytotoxic effect was not always observed. There was a decrease of 21–31% in MTT assay and 38–56% in NRU assay (p < 0.003). There was a dose-response relationship between the amount of cigarettes and lysosomal viability; the correlation was higher for cotton samples (r = −0.843, p < 0.001). As a dose-response relationship was found only in NRU assay, this test may be a more suitable choice rather than the MTT assay. Paper and wipe sampling can be reliable markers of tobacco smoke contamination. Moreover, these materials, if properly prepared, can be used as substrate providers to perform cellular assays.

Sites sobre drogas de abuso: recursos para avaliação

Health professionals should be encouraged to gain independence in the search for, selection, and use of information to solve daily problems. Furthermore, they should be encouraged to reflect on the events that were experienced, to pursue the reconstruction of the path that was followed, and to give them a new meaning. This article presents a learning experience graduate students had during the process of evaluating Brazilian sites on drugs of abuse. After ascertaining the great amount of information on drugs of abuse and the lack of criteria in discussing them on the Internet, a research process got underway that triggered the preparation of a protocol to assist in the process of selecting Brazilian sites that address the drugs of abuse issue.

Cellular effects of thirdhand tobacco smoke from smokers’ homes

Abstract After the cigarette is extinguished, many toxic compounds remain in the environment and accumulate in the air or on surfaces. This exposure is termed thirdhand smoke (THS) and its risks are poorly known. The aim of the study was to evaluate the cellular effects of THS from smokers’ homes. Papers were placed in nine smoker’s home and three nonsmoker’s homes. An area equivalent to the paper size was cleaned with a cotton wipe. A549, Hep-2 and 3T3 cells were exposed to THS for 24 h and cellular functions were assessed by MTT, neutral red (NR) reuptake and trypan blue exclusion assays. High levels of nicotine were found in samples from smokers’ homes. Cellular proliferation was similar in almost all samples after THS exposure. Few changes in the cellular functions were observed, mainly higher mitochondrial activity, in paper samples. We are able to detect markers of THS collected from smokers’ homes, but a clear evidence of cellular toxicity cannot be demonstrated by the present assays. This is the first study to evaluate the cellular effects of THS samples collected from smokers’ homes.

Assessment of factors related to smokers' adherence to a short-term support group for smoking cessation: a longitudinal study in a developing country

Objective: The aim of this study was to determine which individual characteristics of smokers are associated with their adherence to a support group for smoking cessation. Methods: Smokers from Porto Alegre, Brazil, were invited to participate in a support group for smoking cessation consisting of four weekly sessions. Demographic data, smoking history, presence of tobacco-related diseases, severity of nicotine dependence, stage of motivation, and symptoms of anxiety and depression were evaluated at baseline. Adherence was defined as attendance at group sessions and was measured at the second and fourth sessions of the program. Results: The study recruited 167 smokers who attended the first meeting and met criteria for admission to the study. One hundred and two of the participants returned to the second session and only 55 of those who attended the first meeting completed the four-week program. For immediate adherence (second session), adult smokers over the age of 35 were more likely to adhere to the treatment (p = 0.004), whereas smoking higher numbers of cigarettes per day was associated with lower adherence to attendance at group meetings (p = 0.031). For final adherence (fourth session), only minimal level symptoms of anxiety were associated with a higher likelihood of adherence (p = 0.02). Conclusions: Older smokers, those who smoked fewer cigarettes per day, and those with lower levels of anxiety exhibited higher rates of adherence to a smoking cessation support group.

Tobacco chippers seeking assistance for smoking cessation: a case series

OBJECTIVE To describe a series of cases of tobacco chippers (TCs) who sought assistance for smoking cessation. DESCRIPTION OF CASES We describe the case of three TCs who participated in a free quit-smoking support group. Smokers answered self-administered questionnaires covering sociodemographic data, smoking history, nicotine dependence, perceived health status, and motivational stages. Of the 183 smokers enrolled in the program, three (1.6%) were considered TCs. All three were women and reported alcohol use, although at low frequencies. Two of them reported the presence of potentially tobacco-related disease. All TCs presented light nicotine dependence and reported difficulties quitting smoking. All dropped out after the first group meeting. COMMENTS This study describes a subgroup of smokers not extensively described in the literature: TCs seeking assistance for smoking cessation, with some degree of nicotine dependence, and who do not adhere to regular group therapy. Interventions aimed at this population may be an interesting strategy for smoking cessation.