Desak Made Malini

Malformasi Rangka Fetus Tikus Wistar Akibat Efek Teratogenik Insektisida Endosulfan (SKELETAL MALFORMATION OF WISTAR RAT FETUSES DUE TO THE TERATOGENIC EFFECT OF ENDOSULFAN INSECTICIDE)

Endosulfan an organochlorine insecticide that is commonly used even though it has been banned due to its toxic and teratogenic effect. This study aims to determine the effect of orally endosulfan exposure to pregnant rat (Rattus norvegicus) at day 6-15th of gestation period to the foetus skeletal malformation. A Completely Randomized Design (CRD) by endosulfan dosages: 0 (control); 0.083; 0.190; 0.440; 1.000 mg/ kg BW/day with 5 replicates was applied. At 20 of the gestational period, the rats were sacrificed, their reproductive organs and the fetal skeletal malformation were observed using Alizarin red S method. The results of one-way ANOVA test showed that endosulfan exposure did not significantly affect the pregnancy outcomes ie. female rats weight gain, the numbers of implanted foetus, foetal body weight and length, respectively. Malformation of foetus implantation, foetus size and foetus skeletal occured in the endosulfan exposure groups. Fisher’s exact test results showed a significant difference between the control group and the endosulfan exposure group on the number of foetuses that have abnormalities in the number of skeletons of the sternum, fore and hind paws. The exposure of endosulfan at 0.083-1.000 mg / kg BW / day in pregnant rats caused skeletal malformations of the foetus ie. decreased in the number on sternum, foreand hind paw bones.