Devita Tetriana

Evaluation on The Supplementation of Fermented Garlic Extract in Protecting Human Lymphocytes In Vitro From The Negative Impact of Gamma-Rays

Garlic ( Allium sativum ) contains a wide range of phytocompounds that produce various responses in human body. However the knowledge on the potential of fermented form of garlic in protecting negative impacts of radiation is very limited. In this research in vitro efficacy of fermented garlic in protecting negative impact of gamma ray was studied using cytogenetic test. A set of culture of human lymphocytes was irradiated with 60 Co gamma rays at dose of 2 Gy (dose rate of 2 Gy/min) and fermented garlic extract at four working concentrations of 0, 125, 250 and 500 mg/mL were added to these cells and then were incubated at 37 o C for 48 hrs. Colcemid was added at 3 hr before harvest to collect metaphase cells and it was done by standard methodology for cytogenetic analysis. The fermented garlic extract significantly (p<0.05) did not exhibited antigenotoxic effect of gamma rays and its effectiveness was same as in control (without extract treatment) group. In contrary all concentration of chemicals (125, 250 and 500 mg/mL) were seemingly tend to induce higher number of dicentric and fragment chromosomes than control under microscopic observation. Mitotic index of the cell that was determined with programmed metaphase finder also did not influenced by garlic addition. It was concluded that aqueous garlic extract did not possesses its efficacy in protecting impact of ionizing radiation.

Assessment of DNA damage in lymphocytes of Mamuju (a high background radiation area) inhabitants using alkaline single cell gel electrophoresis

Mamuju in Indonesia has a highest average dose rate and considered as a high background radiation area (HBRA) in Indonesia. Here in this research a preliminary study to evaluate DNA damage using the alkaline comet assay in inhabitants of Takandeang village, Mamuju, was conducted. Blood samples from 55 healthy donors living in Takandeang village and 22 control samples were obtained and processed into the assay. Results showed that there were insignificant differences of comet tail length (TL) and tail moment (TM) between Takandeang village inhabitants and control samples (p = 0.578, 0.174). Regression analysis revealed that the DNA damage was increased with age in control samples, even though the correlation was not significant (p > 0.05). In contrast a significant negative correlation (p = 0.02) was observed in Takandeang inhabitants. Results found in this research should be validating in a larger study using more samples from Mamuju.