Dewi Langlet

Survival, Reproduction and Calcification of Three Benthic Foraminiferal Species in Response to Experimentally Induced Hypoxia

An experiment was conducted to test the survival rates, growth (calcification), and reproduction capacities of three benthic foraminiferal species (Ammonia tepida, Melonis barleeanus and Bulimina marginata) under strongly oxygen-depleted conditions alternating with short periods of anoxia. Protocols were determined to use accurate methods (1) to follow oxygen concentrations in the aquaria (continuously recorded using microsensors), (2) to distinguish live foraminifera (fluorogenic probe), (3) to determine foraminiferal growth (calcein-marked shells and automatic measurement of the shell size). Our results show a very high survival rate, and growth of A. tepida and M. barleeanus in all experimental conditions, suggesting that survival and growth are not negatively impacted by hypoxia. Unfortunately, no reproduction was observed for these species, so that we cannot draw firm conclusions on their ability to reproduce under hypoxic/anoxic conditions. The survival rates of Bulimina marginata are much lower than for the other two species. In the oxic treatments, the presence of juveniles is indicative of reproductive events, which can explain an important part of the mortality. The absence of juveniles in the hypoxic/anoxic treatments could indicate that these conditions inhibit reproduction. Alternatively, the perceived absence of juveniles could also be due to the fact that the juveniles resulting from reproduction (causing similar mortality rates as in the oxic treatments) were not able to calcify, and remained at a propagule stage. Additional experiments are needed to distinguish these two options.

Millimeter-scale alkalinity measurement in marine sediment using DET probes and colorimetric determination.

Constrained DET (Diffusive Equilibration in Thin films) probes equipped with 75 sampling layers of agarose gel (DGT Research(©)) were used to sample bottom and pore waters in marine sediment with a 2 mm vertical resolution. After retrieval, each piece of hydrogel, corresponding to 25 μL, was introduced into 1 mL of colorimetric reagent (CR) solution consisting of formic acid and bromophenol blue. After the elution/reaction time, absorbance of the latter mixture was read at 590 nm and compared to a calibration curve obtained with the same protocol applied to mini DET probes soaked in sodium hydrogen carbonate standard solutions. This method allows rapid alkalinity determinations for the small volumes of anoxic pore water entrapped into the gel. The method was assessed on organic-rich coastal marine sediments from Thau lagoon (France). Alkalinity values in the overlying waters were in agreement with data obtained by classical sampling techniques. Pore water data showed a progressive increase of alkalinity in the sediment from 2 to 10 mmol kg(-1), corresponding to anaerobic respiration in organic-rich sediments. Moreover, replicates of high-resolution DET profiles showed important lateral heterogeneity at a decimeter scale. This underlines the importance of high-resolution spatial methods for alkalinity profiling in coastal marine systems.