Dibyendu Bandyopadhyay

111Indium-tropolone labeled human PMNs: a rapid method of preparation and evaluation of labeling parameters.

Pure polymorphonuclear leukocytes (PMNs) have been isolated from a small amout of human blood by a single-step density gradiant centrifugation method using a commercially available Ficoll-Hypaque mixture of density 1.114. The cells were labelled with [111]Intropolone in both buffer and plasma. Cell viability, ability to generate superoxide anion, and chemotaxis were found to be unaltered both before and after labeling. The optimum tropolone concentration for labeling was found to be 1 × 10−4 M. Labeling efficiency was higher at 37°C than at room temperature. Compared to [111In]oxine, tropolone preparation both in buffer and plasma resulted in consistently higher yields. Preliminary experiments of in vivo cell viability of the labeled PMNs were carried out in rabbits. The ability of the cells to localize in experimentally produced inflammatory lesions was found to be intact. The method of cell separation and labeling described has been found to be simple and rapid and could easily be incorporated in routine nuclear medicine laboratory practice.

A multi-user real time inventorying system for radioactive materials : A networking approach

A computerized system for radioisotope management and real time inventory coordinated across a large organization is reported. It handles hundreds of individual users and their separate inventory records. Use of highly efficient computer network and database technologies makes it possible to accept, maintain, and furnish all records related to receipt, usage, and disposal of the radioactive materials for the users separately and collectively. The systems central processor is an HP-9000/800 G60 RISC server and users from across the organization use their personal computers to login to this server using the TCP/IP networking protocol, which makes distributed use of the system possible. Radioisotope decay is automatically calculated by the program, so that it can make the up-to-date radioisotope inventory data of an entire institution available immediately. The system is specifically designed to allow use by large numbers of users (about 300) and accommodates high volumes of data input and retrieval without compromising simplicity and accuracy. Overall, it is an example of a true multi-user, on-line, relational database information system that makes the functioning of a radiation safety department efficient.

A computerized system for control and management of radionuclide inventory: application in nuclear medicine.

An interactive computerized system for radioisotope management and instantaneous inventory is reported. The system is capable of handling operations such as filing, nuclear imaging and disposing of various radionuclides. All radiopharmaceutical transactions are achieved with the aid of a Prime 300 mini-computer of 192K words of high speed semi-conductor memory and over 120 mega bytes of disk storage. The system automatically corrects for the appropriate decay, monitors and updates the storage file after every subsequent study. The performed study is recorded in a special file, together with the time and data retrieved from the computers real time clock at the time of the entry. The system provides an organized and complete bookkeeping of all records concerning radionuclide transactions. It is found to be simple, efficient, highly versatile, and drastically reduces the time of operation and errors in handling the radioisotope inventory.

Biodistribution of Indium-III Dihematoporphyrin Ether in Papillomas and Body Tissues and its Relevance to Photodynamic Therapy

Hematoporphyrin derivative (HPD) and its newly purified form, dihematoporphyrin ether (DUE) have been shown to selectively localize in malignant tissues and virally induced papillomas (Sliikowitz et.al.). Its use as a probe to distinguish tumors from normal tissues has been largely based on its fluorescence when activated by ultraviolet light. These findings are largely subjective, and a direct correlation to its use as a photosensitizing agent to selectively kill transformed cells when activated by an appropriate wavelength of tight (630 nm) could not be made. We labeled DUE with Indium-Ill and tracked its biodistribution through CRPV induced papillomas and normal body tissues.

Simultaneous Evaluation of Radiochromatography Strips Using Gamma Camera Interfaced to a Computer

Chemical quality control of 99”Tc-radiopharmaceuticals is routinely performed by the instant thin-layer chromatographic method’ before the doses are dispensed to the patient. The developed chromatograms are manually cut at a preset cut-line, and each part is counted for radioactivity either in a dose calibrator or in a well counter. To increase the accuracy of measurements, the evaluation of intact chromatograms is essential. The use of a gamma camera equipped with a proper collimator and interfaced to an appropriate computer system is a unique way of analyzing the ~hromatograrns.2~ In this study, multiple radiochromatograms of different kit preparations of 99mTc-radiopharmaceuticals were evaluated simultaneously using the gamma camera technique, and results were compared to that obtained by the conventional “cut and count” procedure.