Dibyendu Chakraborty

Physical methods of gene transfer: Kinetics of gene delivery into cells: A Review

The ability to introduce isolated DNA into cells has tremendous influence on advances of molecular biology. Recently, with the development of attractive strategies for gene therapy, successful gene delivery has gained importance once again and become a major challenge in this field. During the past decades, a wide repertoire of gene transfer techniques has evolved. The intentional introduction of recombinant DNA molecules into a living organism can be achieved in many ways. The array of methods available to move DNA into the nucleus provides the flexibility necessary to transfer genes into cells as physically diverse are Microinjection, Biolistic gene transfer, Electroporation, Sonoporation, Laser irradiation / Photoporation, Magnetofection, Hydroporation and Impalefection.The purpose of this article is to summarise available physical methods of gene transfer, their principles, advantages and limitations.

Genetic polymorphism of promoter region of lactoferrin gene and its association with mastitis resistance in Jersey crossbred cattle

Lactoferrin, a protein found in variety of body secretions, acts as a defense factor in the mammary gland. Total of 50 samples of blood and milk of Jersey crossbred cattle were collected from the Government Dairy Cattle farm, Belicharana. Genomic DNA was isolated and specific primer was used for the amplification of the promoter region of lactoferrin gene. The PCR product comprised of 115 bp. The PCR product was digested with Taq I restriction enzyme. The amplicon has no restriction site and all the 50 animals revealed only one type of genotype (HH) with respect to Taq I / PCR-RFLP. The same PCR products were subjected to SSCP. Amplicons were initially denatured with denaturing agents and then run on native PAGE which was later silver stained. All the 50 samples revealed one type of banding pattern viz., two bands. Single genotype was present in all animals. Hence promoter region of Lactoferrin gene is monomorphic in crossbred cattle under the present study. Somatic cell count was done by staining milk samples with Newmans stain. A total of 19 milk samples had somatic cell count of less than 1,00000 cells/ml with no history of mastitis were considered as resistant, while other 20 samples having somatic cell count more than 5,00000 cells/ml with mastitis were considered as susceptible. Though variation in response to mastitis resistance was detected, no association was established. The reason being that promoter region of lactoferrin gene was monomorphic with respect to Taq I / PCR-RFLP and PCR-SSCP.

Genomic imprinting in mammals-A review

Genomic imprinting refers to an epigenetic mark that distinguishes parental alleles and results in a monoallelic, parental-specific expression pattern in mammals. The alleles of imprinted genes are marked epigenetically as discrete elements termed imprinting control regions with their parental origin in gametes through the use of DNA methylation, at the very least. Imprinted genes are normally involved in foetal growth and behavioural development. Consequently, aberrant imprinting disturbs development and is the cause of numerous well-known imprinting disorders, including Beckwith-Wiedemann syndrome, Prader-Willi syndrome, Cancer and Angelman syndrome.

Effect of Extreme Seasons on Some Blood Biochemical Parameters in Beetal and Toggenberg Goats of Jammu Region

The influence of extreme environmental conditions on biochemical parameters were studied in 36 healthy adult female (2-4 years of age) Beetal (n=18) and Toggenberg (n=18) goats in Jammu (J&K, India). Blood glucose, total cholesterol, total protein, albumin and blood urea nitrogen (BUN) levels were studied during moderate (autumn), extreme hot (summer) and extreme cold (winter) ambiences. Ambient temperature (0C) and relative humidity (%) were recorded and temperature-humidity index (THI) was calculated. For the season and breed interaction, significantly higher (P<0.01) total protein and cholesterol levels were found in Toggenberg goats in all the seasons. Significantly higher (P<0.01) levels of total protein, albumin and BUN levels were found during summer as compared to autumn and winter seasons. The concentrations of glucose and cholesterol were significantly higher (P<0.01) in winter season. Among the two breeds, all the values were significantly higher (P<0.01) in Toggenberg. Biochemical parameters change in different seasons and THI values were found higher during summer season and is considered as danger status.

Non-Genetic Factors Effecting Reproduction Traits in Rambouillet Sheep

1 Division of Animal Genetics and Breeding, Faculty of Veterinary Sciences and Animal Husbandry Sher-e-Kashmir University of Agriculture Sciences and Technology Shuhama Alustang Kashmir 190006, India 2 Animal Husbandry Department, J&K, India 3 Division of Animal Genetics and Breeding SKUAST Jammu, India 4 Department of Zoology and Biotechnology, Hemwati Nandan Bahuguna Garhwal University Uttrakhand India, 246174 5 Department of Biochemistry University of Kashmir, Jammu and Kashmir, India *Corresponding author

Studies on Genetic and Non-Genetic Factors Affecting Performance Traits of Frieswal Cows.

A total of 601 lactation records of 205 Frieswal cows (Holstein Friesian and Sahiwal crossbred) from 1st to 4th parity over a period of 9 years from 1998 to 2006 maintained at Military farm, Jammu Cantonment (J&K, India) were used to estimate performance of reproduction and production traits and to study the effect of genetic and non genetic factors on performance traits. The overall averages for age at first service (AFS), age at first calving (AFC), gestation length (GL), dry period (DP), calving interval (CI), lactation length (LL), total lactation milk yield (TLMY) and milk yield per day of lactation length (MYDL) were estimated as 20.53 ± 0.44 months, 29.72 ± 0.45 months, 278.43 ± 1.54 days, 116.85 ± 4.40 days, 423.46 ± 6.15 days, 305.05 ± 4.51 days, 3087.00 ± 62.60 kg and 10.10 ± 0.16 kg, respectively. The effect of genetic group of sire was significant on age at first service, age at first calving, calving interval, lactation length, total lactation milk yield and milk yield per day of lactation length whereas effect of period was significant on all reproduction and production traits except for gestation length and lactation length. The effect of parity was significant only on dry period and milk yield per day of lactation length whereas effect of season was non-significant on all reproduction and production traits except for calving interval where it had significant effect.