Didier Fradelizi

Jean Pierre Farcet; Noufissa Oudhriri; M. F. Gourdin; Jacqueline Bouguet; Didier Fradelizi; Felix Reyes

Mitogen-driven T cell proliferation in liquid culture requires accessory cells that cooperate in interleukin 2 production. We have investigated the accessory cell requirement for human lymphocyte colony formation under PHA stimulation. Semisolid medium limits cell-to-cell contact emphasizing the role of cooperating cells both in growth factor production and in triggering events. Culturing at high T cell density demonstrates that accessory cells can be substituted for colony formation by exogenous IL-2. Culturing at low T cell density in the presence of IL-2 also demonstrates that accessory cells are required for activation of a subset of progenitors into IL-2 responsive colony-forming cells. Consequently, T colony progenitors, contained in the E-rosetting cell fraction of peripheral blood, are heterogeneous in their triggering signals: a minor subset is directly inducible by PHA, and a major subset is inducible by PHA in the presence of accessory cells. We found that monocytes and some leukemic B cells support effective accessory function in both colony growth factor production and colony progenitor sensitization.

Salem Chouaib; Lucienne Chatenoud; David Klatzmann; Didier Fradelizi

A Quillet; F Presse; C Marchiol-Fournigault; A Harel-Bellan; M Benbunan; H Ploegh; Didier Fradelizi

Hiro Wakasugi; Jacques Bertoglio; Thomas Tursz; Didier Fradelizi

Salem Chouaib; Jacques Bertoglio; Jean Yves Blay; C. Marchiol-Fournigault; Didier Fradelizi

Annick Harel-Bellan; Mireille Joskowicz; Didier Fradelizi; Harvey Eisen

A Harel-Bellan; J Bertoglio; A Quillet; C Marchiol; Hiro Wakasugi; Z Mishall; Didier Fradelizi

J.David Grausz; Didier Fradelizi; François Dautry; Roger Monier; Pierre Lehn

Hiro Wakasugi; A Harel; Marie-Christine Dokhelar; Didier Fradelizi; Thomas Tursz

B Dugas; Aimé Vazquez; J P Geŕard; Y Richard; M T Auffredou; Jean-François Delfraissy; Didier Fradelizi; Pierre Galanaud