Diego de Córdova Cucco

Vacuum-cooled liquid nitrogen increases the developmental ability of vitrified-warmed bovine oocytes

The objective of this study was to determine the effects of vacuum-cooled liquid nitrogen on the development of vitrified immature (germinal vesicle stage; GV) and mature (metaphase II; MII) bovine oocytes after re-warming. Liquid nitrogen was exposed to either atmospheric pressure or to a vacuum (300mm Hg for 45sec); the latter decreased the temperature of the liquid nitrogen to -200°C. Partially denuded oocytes were vitrified either just after selection (GV) or after 22 hours of in vitro maturation (MII) in TCM 199 medium + 10% of estrous mare serum. For vitrification, oocytes were firstly exposed to an intermediate solution (10% EG + 10% DMSO) for 30sec, followed by the vitrification solution (20% EG + 20% DMSO + 0.5M sucrose) for 20sec. Groups of three or four oocytes were loaded into an open-pulled-straw and directly plunged into liquid nitrogen. Oocytes were subsequently rewarmed by exposure to air (25°C) for 4sec, followed by 5 min exposure to decreasing concentrations (0.3 and 0.15M) of sucrose. Fertilization (Day 0) was done with 2 x 10 6 spermatozoa mL -1 (selected by a swim-up procedure) and incubated for 18 to 22 hours. Presumptive zygotes were cultured at 39oC in fourwell dishes with SOFaaci medium, under 5% CO2 and saturated humidity. Cleavage (Day 2) and blastocyst rates (Day 8) were 33.9 and 4.2%, respectively, for GV stage oocytes at atmospheric pressure, 41.2 and 8.8% for GV oocytes under vacuum, 43.5 and 6.7% for MII oocytes at atmospheric pressure, and 53.6 and 10.6% for MII oocytes under vacuum. In conclusion, vacuum-cooled liquid nitrogen improved developmental rates of vitrified-thawed bovine oocytes.

Trichostrongylus and Haemonchus anthelmintic resistance in naturally infected sheep from southern Brazil

The anthelmintic resistance in small ruminants is a common problem and concern worldwide. The aim of this study was to verify anthelmintic treatment efficacy in naturally infected sheep. This study was conducted on nine herds that used the same anthelmintic management for over a year. In each farm, the animals were divided into two groups: untreated control group (n = 5) and treated (n = 10) according to the number of eggs per gram of feces (EPG). The treatment effect was checked based on EPG results and larval culture performed before treatment and 10 days after treatment. Significant differences were not observed (P > 0.05) on EPG results between untreated and treated groups. The coproculture showed that the animals were infected primarily by Haemonchus spp., Trichostrongylus spp., Teladorsagia spp., Cooperia spp. and Oesophagostomum spp. In all farms, anthelmintic resistance by genera Haemonchus and Trichostrongylus was found, but this resistance varied greatly between farms. Haemonchus spp. showed resistance to closantel, levamisole, and albendazole. Trichostrongylus spp. was shown to be resistant to closantel, levamisole, and albendazole. The drugs tested showed to be efficient against the genera Teladorsagia, Cooperia, and Oesophagostomum. Based on these results, we conclude that the anthelmintic resistance to the tested drugs is a problem present in the farms evaluated.

Interação genótipo-ambiente em rebanhos nelore da Amazônia Legal

Genotype-environment interaction was studied by two different procedures in productive and reproductive traits of Nellore cattle. Data from adjusted weights at 120 (P120), 210 (P210), 450 (P450) days of age, scrotal circumference at 450 days of age (PE450) and age at first calving (IPP) for 211,744 records from Nellore herds located in the Legal Amazon region were used in the analysis. The effect of genotype-environment interaction was studied through heritability estimates and rank correlation, comparing the Legal Amazon animals with the general basis of animals - PMGRN Nellore Brazil. Bi-trait analyses considered P120 as anchor-trait with P210, P450 and PE450 as another one. IPP has been analyzed separately in single-trait analysis and considering GCIPP as fixed and additive and residual effects as random. Estimates of heritability for P120, P210, P450, PE450 and IPP on the data of the Legal Amazon were: 0.20 to 0.49; 0.21; 0.48; 0.45; and 0.21, respectively, and in general data of PMGRN - Nellore Brazil were: 0.23; 0.25; 0.34; 0.43 and 0.11, respectively. Correlations between rank for P120, P210, P450, PE450 and IPP were equal to 0.77; 0.79; 0.82; 0.78 and 0.38, respectively. The analysis of the genotype-environment interaction, through the heritability estimates, showed larger effects on maternal, weight at 450 days of age and age at first calving, whereas the rank correlations showed strong evidence in almost all traits studied.

Occurrence of gastrointestinal parasites in goats from the Western Santa Catarina, Brazil

This study aims to investigate the occurrence of gastrointestinal parasites in goats from the Western Santa Catarina, Brazil. Twenty four farms were analyzed in 17 different municipalities. Animals (n=217) from different production purposes (milk and meat) and age were randomly chosen. Fecal samples were collected from the rectum stored in plastic bottles and transported to the laboratory in portable coolers at 10 °C. The technique of centrifugal flotation with saturated sugar solution was carried out in order to investigate the presence of eggs, cysts, and oocysts of gastrointestinal parasites. In 88.9% of the investigated animals, it was observed that the presence of nematode eggs which belongs to the Strongylida order, after cultivation and larvae identification were identified as Haemonchus spp., Trichostrongylus spp., Teladorsagia spp., Cooperia spp., and Oesophagostomum spp. Eggs of Thysanosoma, Trichuris, Moniezia, and Neoascaris genus were also observed. Additionally, the presence of oocysts of Eimeria spp. and Cryptosporidium spp. as well as cysts of Giardia spp., and Entamoeba spp. were verified. In all the farms evaluated, the animals showed a single or mixed infection, with the highest occurrence of helminths belonging to the Haemonchus and Trichostrongylus genus, as well as the protozoan Eimeria.

Genetic profile of Holstein and Jersey dairy bull lines available in Brazil

As racas de bovinos de leite Holandesa e Jersey estao entre as mais prevalentes no Brasil. Para avaliar as diferencas entre as PTAs (habilidades de transmissao preditas) destas racas e suas linhagens, foram coletadas informacoes de 385 touros Holandes e 82 touros Jersey disponibilizados por nove empresas comercializadoras de semen no Brasil. Tres diferentes linhagens de cada raca foram encontradas. O metodo dos modelos lineares generalizados foi utilizado para a comparacao entre linhagens e entre racas. As duas linhagens da raca Holandesa mais prevalentes apresentaram as maiores PTAs medias para producao de leite (1.061,04 libras e 975,32 libras) e as menores PTAs para a porcentagem de solidos no leite (de -0,05% a -0,0003%) (P<0.05). Estes resultados sugerem a oferta de touros no mercado nacional que sao capazes principalmente de aumentar a producao de leite. A raca Jersey apresentou um coeficiente de endogamia mais elevado (6,62%) que o da raca Holandesa (5,14%) (P<0.05). Embora a raca Holandesa tenha apresentado maiores PTAs para producao de leite e as menores PTAs para a porcentagem de solidos no leite, a vida produtiva da raca Jersey apresentou-se mais longa (1,17 meses) (P<0.05) que a da raca Holandesa (0,40 meses). Houve uma importante variabilidade do material genetico disponivel, permitindo a escolha do semen mais adequado para cada sistema de producao. Faz-se necessario conhecer o perfil genetico dos touros disponibilizados para inseminacao artificial no Brasil, com vistas ao conhecimento e direcionamento do padrao genetico dos bovinos leiteiros no pais.