Diego Montalti

Physiological and biochemical aspects of the avian uropygial gland.

This review discusses different aspects of the uropygial gland of birds. The gland exhibits a striking morphological diversity in size, shape and presence/absence of tufts of feathers. It was shown that acidic mucins, neutral lipids, glycolipids and phospholipids are normal components of secretion. Several morphological and physiological aspects of the gland were studied on Rock Pigeon Columba livia Gmelin, 1879. The amount of the uropygial gland secretion, its lipid content and fatty acids profile were determined. The extracted lipid mixture contained of C14 to C20 fatty acids, mostly unsaturated; the saturated fatty acids were mainly 14:0, 16:0 and 18:0. No correlation was found between the size of the gland and the aquatic/terrestrial nature of the species. Ablation of the gland did not affect survival, body weight, feeding rate and serum cholesterol, total lipids or calcium levels after 32-120 days. The possible role of the gland in the protection against lipophilic compounds was discussed. The function of the gland is still a subject of controversy. It is accepted that its secretion confers water-repellent properties on the feather coat and maintain the suppleness of the feathers. Other physiological roles of the gland secretion may be associated to pheromone production, control of plumage hygiene, thermal insulation and defence against predators. Concerning the endocrine regulation of the uropygial function, there is scarce information presenting evidence for steroid regulated mechanisms.

Trophic interactions between brown and south polar skuas at Deception Island, Antarctica

It is broadly accepted that the brown skua (Stercorarius antarcticus lonnbergi) competitively excludes the south polar skua (S. maccormicki) from penguin colonies when breeding sympatrically, forcing the latter to feed on marine resources. The purpose of this work was to examine the diets and trophic niche breadths of each species where they co-occur and to determine the degree of overlap. To this end, we analyzed 169 pellets of brown skuas, collected in two different areas (20 individuals), and 152 of south polar skuas, collected in three different areas (18 individuals), on Deception Island, South Shetland Islands, Antarctica, during the austral summer 2000. Pellet analysis often underestimates the amount of easily digestible prey, but allows for comparisons of the relative contributions of different items in the diet. South polar skuas at our study locations consumed seven different food items and had a trophic niche breadth of 0.133 compared to brown skuas that fed on 10 different items and had a trophic niche breadth of 0.078. The niche overlap between the species was 82.1%. Penguins were the principal food source of both species, however, brown skuas fed mostly on chicks, while south polar skuas fed on adults (carcasses). The use of different age classes of penguins as a food source offers an alternative to competitive exclusion, allowing the coexistence of these species on Deception Island.

Seeds in the Diet of the White-rumped Sandpiper in Argentina

Abstract The White-rumped Sandpiper (Calidris fuscicollis) breeds in Alaska and Canada, and during the nonbreeding season migrates to South America. The reported diet of this sandpiper is principally invertebrates; seeds are a very rare item. This species was studied at Guamini Pond, Buenos Aires Province, Argentina, in December 1997. The stomach contents of 23 adults were investigated. The analysis showed that during the sampling period the only food eaten by the White-rumped Sandpiper were seeds. Them were represented by the following families: Caryophylaceae, Chenopodiaceae, Fabaceae, Malvaceae, Polygonaceae, Ulmaceae, Cyperaceae and Poaceae. The seeds ranged between 0.83 and 2.25 mm in length. Stones and fragments of algae were found in all samples, the algae possibly ingested accidentally during feeding. Our findings indicate that the White-rumped Sandpiper forages on different items depending on the area, season and availability, and may be an opportunistic feeder.—The White-rumped Sandpiper ( Calidris fuscicollis ) breeds in Alaska and Canada, and during the nonbreeding season migrates to South America. The reported diet of this sandpiper is principally invertebrates; seeds are a very rare item. This species was studied at Guaminí Pond, Buenos Aires Province, Argentina, in December 1997. The stomach contents of 23 adults were investigated. The analysis showed that during the sampling period the only food eaten by the White-rumped Sandpiper were seeds. Them were represented by the following families: Caryophylaceae, Chenopodiaceae, Fabaceae, Malvaceae, Polygonaceae, Ulmaceae, Cyperaceae and Poaceae. The seeds ranged between 0.83 and 2.25 mm in length. Stones and fragments of algae were found in all samples, the algae possibly ingested accidentally during feeding. Our findings indicate that the White-rumped Sandpiper forages on different items depending on the area, season and availability, and may be an opportunistic feeder. Received 25 October 2002, accepted 29 December 2002.

Diet of South Polar Skua Chicks in Two Areas of Sympatry with Brown Skua

Abstract. The food provided to South Polar Skuas (Stercorarius maccormicki) chicks was determined at two Antarctic sites where South Polar Skuas breed sympatrically with Brown Skuas (S. antarcticus). At Harmony Point on Nelson Island, 16 samples of regurgitated stomach contents were analyzed, all contained remains of penguin chicks (mostly soft tissues), which constituted the bulk of the contents, while only two samples had fish or krill remains. These South Polar Skuas had access to penguin colonies, where they hunted chicks or fed on fresh carcasses. However, at Half Moon Island, only two out of nine samples contained minor amounts of penguin remains while fish were the major dietary resource. The high fish content in their diet may indicate a preference for marine rather than terrestrial resources or be the result of competition with Brown Skuas. The variability in food items evidenced by the differences in regurgitated stomach contents indicates that South Polar Skuas may have a trophic plasticity that allows them to change their feeding habits from one resource to another one according to availability. Such may make them less vulnerable to a reduction in the availability of a particular food item.

Hematology, Biochemistry and Serum Protein Analyses of Antarctic and non-Antarctic Skuas

Abstract. Determination of hematological and biochemical parameters provides important data to assess the physiological condition in wild birds. Therefore, to carry out ecophysiology or conservation studies it is essential to establish baseline physiological parameters and how these change with age and life history events. Hematological (hematocrit, hemoglobin and erythrocyte sedimentation) and biochemical (glucose, total lipids and proteins, aspartate and alanine aminotransferase activities and electrolyte concentration) reference values were determined in two Antarctic migratory bird species, the Brown Skua (Stercorarius antarcticus) and South Polar Skua (S. maccormicki), from South Shetland Island during breeding season. Also, hematological data (hematocrit) were determined for non-Antarctic skuas, with Chilean (S. chilensis) and Falkland (S. antarcticus antarcticus) skuas sampled in the Beagle Channel islands (Tierra del Fuego Province) and Viana Island (Chubut Province), Argentina, respectively. Differences between adult Antarctic skua species were observed in hemoglobin, erythrocyte sedimentation, total lipids and aspartate aminotransferase activity. In addition, age-related differences in Antarctic skuas in hematocrit, hemoglobin, glucose and total protein values were observed. Serum reference protein fractions (Albumin, &agr;1, &agr;2, &bgr; and &ggr; globulins) were assessed by electrophoresis for Antarctic and non-Antarctic skuas. Similar protein patterns were observed between South Polar and Chilean skuas as well between Falkland Skua and Brown Skua. The differences between adult sympatric Antarctic skuas may be related to their nutritional status and species-specific migrations, feeding habits and the differential use of the breeding niches, while the age variation may be related to physiological development processes in chicks or to the energy expenditure in adults during breeding.

Morphology and histology of the uropygial gland in Antarctic birds: relationship with their contact with the aquatic environment?

Abstract. The uropygial gland is morphologically different in diverse bird species. This gland was macroscopically and microscopically examined in penguins, storm petrels and skuas. In all the studied species, the gland showed a connective tissue capsule and one papilla. A negative relationship was observed between the relative glandular mass and the body mass, being highest in petrels (small glands) and lowest in penguins (large glands). Birds that spend much time in water (penguins) have gland characteristics related to a continuous, but not stored, secretion, such as straight adenomers, the presence of abundant elastic fibres in the connective tissue and the absence of a primary storage chamber. Instead, birds that have less contact with water (storm petrels) have a gland with much more tortuous adenomers and a small primary storage chamber. The secretory cells showed a positive PAS reaction in all the glandular zones. Therefore, no differences could be seen between the sebaceous and glucogenic zones, as proposed in other birds. These results allow the conclusion that, in aquatic birds, there is no connection between the relative mass of the uropygial gland and the time in contact with water, though the differences found in the histological structure could be related to a different contact with the aquatic environment.

Multiple sex chromosome system in penguins (Pygoscelis, Spheniscidae)

Abstract Penguins are classified in the order Sphenisciformes into a single family, Spheniscidae. The genus Pygoscelis Wagler, 1832, is composed of three species, Pygoscelis antarcticus Forster, 1781, P. papua Forster, 1781 and P. adeliae Hombron & Jacquinot, 1841. In this work, the objective was to describe and to compare the karyotypes of Pygoscelis penguins contributing genetic information to Sphenisciformes. The metaphases were obtained by lymphocyte culture, and the diploid number and the C-banding pattern were determined. P. antarcticus has 2n = 92, P. papua 2n = 94 and P. adeliae exhibited 2n = 96 in males and 2n = 95 in females. The difference of diploid number in P. adeliae was identified as a multiple sex chromosome system where males have Z1Z1Z2Z2 and females Z1Z2W. The C-banding showed the presence of a heterochromatic block in the long arm of W chromosome and Z2 was almost entirely heterochromatic. The probable origin of a multiple system in P. adeliae was a translocation involving the W chromosome and the chromosome ancestral to Z2. The comparison made possible the identification of a high karyotype homology in Sphenisciformes which can be seen in the conservation of macrochromosomes and in the Z chromosome. The karyotypic divergences in Pygoscelis are restricted to the number of microchromosomes and W, which proved to be highly variable in size and morphology. The data presented in this work corroborate molecular phylogenetic proposals, supporting the monophyletic origin of penguins and intraspecific relations.

Winter diet of Great Pampa-finches Embernagra platensis in Guaminí Lagoon, Argentina

Abstract. The diet of the Great Pampa-finch includes seeds rather than insects. Our objective was to study its diet during the winter season and, whenever possible, to record differences between the sexes in diet patterns. This species was studied at Guamini Lagoon, Buenos Aires Province, Argentina. The stomach content of 38 adult birds was investigated. The analysis shows that the food eaten by E. platensis consisted of seeds (60%) (Chenopodiaceae, Asteraceae, Ciperaceae and Poaceae) and insects (39%) (Bellostomatidae, Dytiscidae, Curculionidae, Coccinelidae, Formicidae and Apidae). Males ate more insects and, overall, more items than females. These, in turn, consumed a greater amount of plants. Niche breadth values were 2.9 for males and 3.1 for females. The seeds encountered were those of plant species typically associated with the pampas.

Histological and histochemical study of the uropygial gland of chimango caracara (Milvago chimango vieillot, 1816)

The uropygial glands of birds are sebaceous organs that contribute to the water-repellent properties of the feather coat. We studied the histological and histochemical characteristics of the uropygial gland of chimango caracara using hematoxylin and eosin (H & E), Gomori´s trichrome, orcein, Gomori´s reticulin, periodic acid-Schiff (PAS), Alcian blue (AB) and a variety of lectins. The gland is composed of two lobes and a papilla with 20 downy feathers. It is surrounded by a capsule of dense connective tissue that contains elastic, reticular and smooth muscle fibers. The papilla is delicate and has two excretory ducts. The gland mass relative to body mass was 0.143%. Both adenomer cells and their secretions were stained with Sudan IV, PAS and AB, and were positive for numerous lectins that indicated the presence of lipids and carbohydrates. Immunohistochemical techniques to detect PCNA confirmed cell proliferation in the basal stratum of the adenomer cells. The lipids and glycoconjugates secreted by the uropygial gland serve numerous functions including protection against microorganisms.

Parasitism by Botflies Philornis Sp. on European Starlings Sturnus vulgaris, an Exotic Bird in Argentina

Summary. We studied the parasitism of the exotic European starling Sturnus vulgaris by native botflies Philornis spp. in Argentina. We installed thirty nest boxes in the northeastern Buenos Aires province in the 2010–2011 breeding season. In the first brood, subcutaneous larvae of Philornis (Muscidae) parasitised 34 nestlings (89.4%) of 11 clutches (91.6%) and only three nestlings fledged. In the second brood, Philornis parasitised 15 (48.3%) nestlings of seven clutches (70%) and all the nestlings died. Compared to the mortality of other Neotropical birds parasitised by Philornis, the mortality in European starling nests is the highest found in the region. Our results show that the studied population of European starling suffers a high level of parasitism by Philornis, although this factor was not directly associated with the high mortality of starling nestlings. The fact that most non-parasitised nestlings also died indicates that other factors are also affecting nestling survival. Experiments that allow us to isolate the effect of Philornis from other variables would be needed to evaluate the impact of botfly larvae on starling nestlings.