Diego Segatto Blaya

Analysis of the Systemic Effect of Red and Infrared Laser Therapy on Wound Repair

OBJECTIVE To evaluate, using histological analysis, the systemic action and repair process of wounds produced on the back of rats and treated with red, infrared, or both lasers applied directly or indirectly to the wounds. BACKGROUND DATA Skin tissue repair and wound healing are complex processes that involve a series of dynamic events. Many benefits are associated with biomodulation using laser therapy. METHODS Thirty-six male Wistar rats were divided into four groups: control (without laser), red laser (aluminium gallium indium phosphide (AlGaInP); lambda=685 nm; phi=0.0314 cm2; CW; P=30 mW; D=20 J, time of irradiation=667 sec), infrared laser (gallium-aluminum-arsenide (GaAlAs): lambda=830 nm; phi=0.0314 cm2; CW; P=50 mW; D=20 J, time of irradiation=401 sec), and both lasers (infrared laser: GaAlAs; lambda=830 nm; phi=0.0314 cm2; CW; P=50 mW; D=10 J, time of irradiation=201 sec+red laser: AlGaInP; lambda=685 nm; phi=0.0314 cm2; CW; P=30 mW; D=10 J, time of irradiation=334 sec; total dose=20 J). Three subgroups were formed according to observation time points. Three wounds were produced on the back of each animal. Only the wound closest to the head was irradiated in the experimental groups. For the evaluation of skin reaction and wound healing, three animals of each group were killed at 3, 5, and 7 days postoperatively. The irradiation protocol established 48-hour intervals between applications, with the first application immediately after the surgical procedure. RESULTS In the red and infrared laser group, healing was more advanced in the wound located furthest from the point of laser application. The most effective healing of a proximal wound was verified in the control group on the 7th postoperative day. CONCLUSION The combined application of red and infrared lasers resulted in the most evident systemic effect on the repair of skin wounds produced in rats.

Patient's perception on mini-screws used for molar distalization

OBJETIVO: O objetivo desse estudo foi avaliar e comparar a intensidade de dor, os efeitos colaterais e o desconforto sentido pelo paciente relacionado a colocacao do mini-implante, durante a mecânica e na remocao do mesmo, durante a distalizacao de molares no tratamento ortodontico. METODOS: A amostra foi composta por 30 pacientes adultos com uma media de idade de 30 anos, com maloclusao Classe II subdivisao direita ou esquerda. Um mini-implante foi instalado em cada paciente, no arco superior para conseguir a distalizacao de molar. Os pacientes receberam um questionario a respeito de sua opiniao sobre o tratamento. RESULTADOS: Noventa por cento dos pacientes escolheram colocar os mini-implantes ao inves de realizar extracao de molares (camuflagem ortodontica), fazer o uso de um aparelho extraoral (Aparelho extraoral de Kloehn) ou fazer uso de qualquer outro tratamento alternativo para classe II. A ulcera aftosa foi o efeito colateral com a maior incidencia apos a insercao do mini-implante (30%). O maior desconforto foi sentido durante a infiltracao anestesica (27%), embora 23% relataram nao ter sentido desconforto durante o tratamento. Um total de 83% dos pacientes nao relatou dor durante o tratamento, o que pode ser associado com o grau de satisfacao com o tratamento (100%) e a recomendacao desse procedimento a outros pacientes (100%). CONCLUSAO: Os mini-implantes foram bem aceitos pelos pacientes e foram eficientes para a distalizacao de molares quando utilizados em associacao com o tratamento ortodontico para correcao da maloclusao de Classe II.

Titanium alloy miniscrews for orthodontic anchorage: an in vivo study of metal ion release

PURPOSE: To examine and compare the levels of several metal ions released in the saliva of patients with orthodontic appliances, at different time points before and after insertion of a miniscrew. METHODS: Saliva of patients (n=20) was collected at four time points: before miniscrew placement (T1), 10 minutes (T2), 7 days (T3) and 30 days after miniscrew placement (T4). The salivary samples were analyzed by inductively coupled plasma mass spectrometry (ICP-MS) and inductively coupled plasma optical emission spectrometry (ICP-OES). The release of nine different metal ions was observed: titanium (Ti), zinc (Zn), chromium (Cr), nickel (Ni), iron (Fe), copper (Cu), aluminum (Al), Vanadium (V) and cobalt (Co). Data were analyzed by descriptive statistics. Salivary metal concentrations from different time points of miniscrew treatment were compared using Wilcoxon paired tests (a=5%). RESULTS: At time point T4, there was a quantitative increase in the salivary concentration of Cu, Ti, V, Zn, as well as a quantitative decrease in the salivary concentration of Al, Co, Cr, Fe, Ni, when compared with T1. CONCLUSION: It can be concluded that the placement of fixed orthodontic appliances associated with miniscrews does not lead to an increase of salivary metal ion concentrations.