Dimitre G. Ouzounov

All-optical switching on a silicon chip

We present an experimental demonstration of fast all-optical switching on a silicon photonic integrated device by employing a strong light-confinement structure to enhance sensitivity to small changes in the refractive index. By use of a control light pulse with energy as low as 40 pJ, the optical transmission of the structure is modulated by more than 97% with a time response of 450 ps.

Compact and flexible raster scanning multiphoton endoscope capable of imaging unstained tissue

We present a compact and flexible endoscope (3-mm outer diameter, 4-cm rigid length) that utilizes a miniaturized resonant/nonresonant fiber raster scanner and a multielement gradient-index lens assembly for two-photon excited intrinsic fluorescence and second-harmonic generation imaging of biological tissues. The miniaturized raster scanner is fabricated by mounting a commercial double-clad optical fiber (DCF) onto two piezo bimorphs that are aligned such that their bending axes are perpendicular to each other. Fast lateral scanning of the laser illumination at 4.1 frames/s (512 lines per frame) is achieved by simultaneously driving the DCF cantilever at its resonant frequency in one dimension and nonresonantly in the orthogonal axis. The implementation of a DCF into the scanner enables simultaneous delivery of the femtosecond pulsed 800-nm excitation source and epi-collection of the signal. Our device is able to achieve a field-of-view (FOVxy) of 110 μm by 110 μm with a highly uniform pixel dwell time. The lateral and axial resolutions for two-photon imaging are 0.8 and 10 μm, respectively. The endoscope’s imaging capabilities were demonstrated by imaging ex vivo mouse tissue through the collection of intrinsic fluorescence and second-harmonic signal without the need for staining. The results presented here indicate that our device can be applied in the future to perform minimally invasive in vivo optical biopsies for medical diagnostics.

Resonant optical interactions with molecules confined in photonic band-gap fibers

We investigate resonant nonlinear optical interactions and demonstrate induced transparency in acetylene molecules in a hollow-core photonic-band-gap fiber at 1.5 mum. The induced spectral transmission window is used to demonstrate slow-light effects, and we show that the observed broadening of the spectral features is due to collisions of the molecules with the inner walls of the fiber core. Our results illustrate that such fibers can be used to facilitate strong coherent light-matter interactions even when the optical response of the individual molecules is weak.

Divided-pulse amplification of ultrashort pulses

We demonstrate an approach to avoid nonlinear effects in amplification. The initial pulse is divided into a sequence of lower-energy identical pulses. The low-intensity pulses are amplified and recombined to create a final pulse.

Delivery of nanojoule femtosecond pulses through large-core microstructured fibers

We investigate femtosecond-pulse propagation through large-core microstructured fibers. Although these fibers are highly multimode, excitation of the fundamental mode is readily achieved, and coupling to higher-order modes is weak even when the fiber is bent or twisted. For prechirped input pulses with energies as large as 3 nJ, pulses as short as 140 fs were produced at the output of the fiber. Such a system could prove to be extremely useful for applications such as in vivo multiphoton microscopy and endoscopy that require delivery of femtosecond pulses and collection of fluorescence.

Soliton pulse compression in photonic band-gap fibers.

We report on pulse compression using a hollow-core photonic band-gap fiber filled with Xe. Output pulses with megawatt peak powers and durations of 50 fs have been generated from 120-fs input pulses. The large third-order dispersion inherent in these fibers degrades the optimal compression ratio and prevents generation of even shorter pulses. Nevertheless, for picosecond input pulses, compression to less than 100 fs is predicted.

In vivo imaging of unstained tissues using long gradient index lens multiphoton endoscopic systems

We characterize long (up to 285 mm) gradient index (GRIN) lens endoscope systems for multiphoton imaging. We fabricate a portable, rigid endoscope system suitable for imaging unstained tissues, potentially deep within the body, using a GRIN lens system of 1 mm diameter and 8 cm length. The portable device is capable of imaging a ~200 µm diameter field of view at 4 frames/s. The lateral and axial resolution in water is 0.85 µm and 7.4 µm respectively. In vivo images of unstained tissues in live, anesthetized rats using the portable device are presented. These results show great promise for GRIN endoscopy to be used clinically.

In vivo imaging of unstained tissues using a compact and flexible multiphoton microendoscope

We use a compact and flexible multiphoton microendoscope (MPME) to acquire in vivo images of unstained liver, kidney, and colon from an anesthetized rat. The device delivers femtosecond pulsed 800 nm light from the core of a raster-scanned dual-clad fiber (DCF), which is focused by a miniaturized gradient-index lens assembly into tissue. Intrinsic fluorescence and second-harmonic generation signal from the tissue is epi-collected through the core and inner clad of the same DCF. The MPME has a rigid distal tip of 3 mm in outer diameter and 4 cm in length. The image field-of-view measures 115 μm by 115 μm and was acquired at 4.1 frames/s with 75 mW illumination power at the sample. Organs were imaged after anesthetizing Sprague-Dawley rats with isofluorane gas, accessing tissues via a ventral-midline abdominal incision, and isolating the organs with tongue depressors. In vivo multiphoton images acquired from liver, kidney, and colon using this device show features similar to that of conventional histology slides, without motion artifact, in ~75% of imaged frames. To the best of our knowledge, this is the first demonstration of multiphoton imaging of unstained tissue from a live subject using a compact and flexible MPME device.

Thermal emittance and response time measurements of negative electron affinity photocathodes

The thermal emittance and temporal response of a photocathode set an upper limit on the maximum achievable electron beam brightness from a photoemission electron source, or photoinjector. We present measurements of these parameters over a broad range of laser wavelength for two different negative electron affinity (NEA) photocathodes. The thermal emittance of NEA GaAs and GaAsP has been measured by two techniques—a measurement of the beam size downstream from a solenoid, whose strength was varied, and a double slit transmission measurement—for different laser spot sizes and shapes. The effect of space charge on the beam spot size allows a good estimation of the photoemission response time from these cathodes. Both cathodes show a subpicosecond response for laser wavelengths shorter than 520 nm.

In vivo three-photon imaging of activity of GCaMP6-labeled neurons deep in intact mouse brain

High-resolution optical imaging is critical to understanding brain function. We demonstrate that three-photon microscopy at 1,300-nm excitation enables functional imaging of GCaMP6s-labeled neurons beyond the depth limit of two-photon microscopy. We record spontaneous activity from up to 150 neurons in the hippocampal stratum pyramidale at ∼1-mm depth within an intact mouse brain. Our method creates opportunities for noninvasive recording of neuronal activity with high spatial and temporal resolution deep within scattering brain tissues.