Dinanath Gaikwad

Enhancement in in vitro anti-angiogenesis activity and cytotoxicity in lung cancer cell by pectin-PVP based curcumin particulates

The aim of this work was to prepare pectin-poly (vinyl pyrrolidone) [PVP] based curcumin particulates to enhance the anticancer potential of curcumin, solubility and allow its localized controlled release. Pectin-PVP based curcumin particulates (PECTIN-PVP CUR) were prepared by spray drying technique in different ratios and were evaluated for surface morphology, micromeritics, flowability, particle size, drug content, in vitro dissolution, inhalable fraction, anti-angiogenesis/angiolysis and cytotoxicity. Results of micromeritic properties, Carrs index, Hausners ratio and angle of repose were satisfactory. The batch CP3 was considered as optimum, due to excellent flowability, acceptable aggregation and enhanced solubility. The particle size and size distribution data of selected batch CP3 showed 90% of curcumin particulates having size less than 2.74μm, which may deposit to lungs. Twin Impinger studies showed that 29% of respirable fraction was generated, which could be directly delivered to lungs. The in vitro dissolution data showed many fold increase in dissolution rate. Angiolytic activity and MTT assay of PECTIN-PVP CUR have demonstrated enhancement in the anti-tumor potential, compared to curcumin alone. Altogether, PECTIN-PVP CUR were found suitable for local delivery and enhance its anticancer potential of curcumin.

DEVELOPMENT OF PARTICULATE MUCOADHESIVE GEL FOR INTRANASAL DELIVERY

Objective: The objective of this research work was to develop mucoadhesive particulates gel of Propranolol HCl for intranasal delivery. Method: Drug loaded mucoadhesive particulates were prepared by spray drying technique using polymers such as HPMC K100 and Carbopol 934P. Batches were prepared according to 3 2 factorial designs. Result: The mucoadhesive particulates prepared were evaluated for different parameters like drug content, entrapment efficiency, mucoadhesive strength and in vitro drug release. IR, XRD and DSC study revealed that there were no interaction occurs between drug and excipients and confirming reduction in crystallinity. The swelling index and encapsulation efficiency was found to be (0.9266%), (97.44%), angle of repose, Carr’s compressibility index falls in acceptable limits. At the end of 10 hr optimized batch showed 90.23 % drug release and followed zero order release kinetics. Conclusion: Conclusion from result of the studies such as increase in the concentration of polymers contributed in drug release retardation. Although, prepared formulation of nasal administration can be a value addition in treatment for heart diseases like angina pectoris, myocardial