Dinku M. Endale

The relationship of land use practices to surface water quality in the Upper Oconee Watershed of Georgia

On a watershed scale, geospatial information can be used to identify water resources that are least buffered from contamination. Implementing conservation practices at these locations may accelerate the process of increasing a watersheds ability to support its designated uses. The Upper Oconee Watershed of Georgia contains land areas devoted to poultry, dairy, and beef production. Within these historically agricultural lands, urbanization is proceeding rapidly around existing cities. Agricultural production practices are concentrated in the watershed with poultry in the headwaters area and dairy near a major lake (Lake Oconee). The objective of this research was to relate data sets representing surface water quality at selected sites throughout the watershed to the predominant land use in that portion of the watershed. The location of 550 poultry operations in the headwaters of the Upper Oconee Watershed, away from the city of Athens GA, has minimized conflicts between agricultural and urban interests. Phosphorus, nitrogen, and fecal coliform bacteria were high near the poultry production area, but were reduced within the watershed prior to reaching the intake for the municipal water supply. Athens had a large impact on surface water quality and approximately doubled the amount of phosphorus and nitrogen in the Oconee River. The Oconee River contributed approximately 70% of the water flowing to Lake Oconee. The residents of Lake Oconee have noted the 30 dairies located west of the lake impacting two relatively minor creeks flowing to the lake. These two creeks make up approximately 2.5% of the flow to the lake, but the proximity of the dairies to the lake makes losses of phosphorus, nitrogen, and fecal bacteria apparent in water samples. Fecal coliform numbers were elevated in some creeks with little agricultural or urban development. To test alternative microbial assays, surface water from a grazed watershed was compared to water from a wooded watershed. Assays for enterococci and E. coli may provide a better test for fecal contamination and allow differentiation between natural areas and areas impacted by grazing animals. Analysis of the Upper Oconee Watershed identified agricultural impacts and areas that should be priorities for natural resource management to reduce agricultural non-point source pollution. Focusing conservation efforts at these locations may prevent agricultural-urban conflict. However, the data also indicate that municipal sources of nutrients and fecal bacteria must be reduced to make significant progress in the watershed.

Assessing Indices for Predicting Potential Nitrogen Mineralization in Soils under Different Management Systems

A reliable laboratory index ofN availability would be useful for making N recommendations, but no single approach has received broad acceptance across a wide range of soils. We compared several indices over a range of soil conditions to test the possibility of combining indices for predicting potentially mineralizable N (N 0 ). Soils (0-5 and 5-15 cm) from nine tillage studies across the southern USA were used in the evaluations. Long-term incubation data were fit to a first-order exponential equation to determine N 0 , k (mineralization rate), and N 0 * (N 0 estimated with a fixed k equal to 0.054 wk -1 ). Out of 13 indices, five [total C (TC), total N (TN), N mineralized by hot KCI (Hot_N), anaerobic N (Ana_N), and N mineralized in 24 d (Nmin_24)] were strongly correlated to N 0 (r > 0.85) and had linear regressions with r 2 > 0.60. None of the indices were good predictors ofk. Correlations between indices and N 0 * improved compared with N 0 , ranging from r = 0.90 to 0.95. Total N and Hush of CO 2 determined after 3 d (Fl_CO2) produced the best multiple regression for predicting N 0 (R 2 = 0.85) while the best combination for predicting N 0 * (R 2 = 0.94) included TN, Fl_CO2 Cold_N, and NaOH_N. Combining indices appears promising for predicting potentially mineralizable N, and because TN and Fl_CO2 are rapid and simple, this approach could be easily adopted by soil testing laboratories.

Influence of cover crops on potential nitrogen availability to succeeding crops in a Southern Piedmont soil

Winter cover crops are essential in conservation tillage systems to protect soils from erosion and for improving soil productivity. Black oat (Avena strigosa Schreb) and oilseed radish (Raphanus sativus L.) could be useful cover crops in the southeastern USA, but successful adoption requires understanding their influence on N availability in conservation tillage systems. Black oat and oilseed radish were compared to crimson clover (Trifolium incarnatum L.) and rye (Secale cereale L.) for biomass production and effects on N mineralization during the summer crop growing season from fall 1998 through summer 2002 near Watkinsville, GA. Rye produced 40 to 60% more biomass, although N contents were less than the other cover crops. Oilseed radish and black oat N contents were similar to crimson clover. Black oat, oilseed radish, and crimson clover C/N ratios were less than 30, whereas rye averaged 39. Amount of N mineralized in 90 days (Nmin90) measured with in situ soil cores was 1.3 to 2.2 times greater following black oat, crimson clover, and oilseed radish than following rye. No differences in Nmin90 were found between black oats, crimson clover, and oilseed radish in 1999 and 2000. The amount of potentially mineralizable N (N0) was not different due to cover crop, but was 1.5 times greater in 2000 and 2002 than in 1999. The rate of N mineralization (k) was 20 to 50% slower following rye than the other three cover crops. Black oat and oilseed radish biomass production and soil N mineralization dynamics were more similar to crimson clover than to rye, which indicates that they could be used as cover crops in the southeast without significant changes in N recommendations for most crops.

Rainfall and tillage effects on transport of fecal bacteria and sex hormones 17β-estradiol and testosterone from broiler litter applications to a Georgia Piedmont Ultisol

Poultry litter provides nutrients for crop and pasture production; however, it also contains fecal bacteria, sex hormones (17beta-estradiol and testosterone) and antibiotic residues that may contaminate surface waters. Our objective was to quantify transport of fecal bacteria, estradiol, testosterone and antibiotic residues from a Cecil sandy loam managed since 1991 under no-till (NT) and conventional tillage (CT) to which either poultry litter (PL) or conventional fertilizer (CF) was applied based on the nitrogen needs of corn (Zea mays L) in the Southern Piedmont of NE Georgia. Simulated rainfall was applied for 60 min to 2 by 3-m field plots at a constant rate in 2004 and variable rate in 2005. Runoff was continuously measured and subsamples taken for determining flow-weighted concentrations of fecal bacteria, hormones, and antibiotic residues. Neither Salmonella, nor Campylobacter, nor antimicrobial residues were detected in litter, soil, or runoff. Differences in soil concentrations of fecal bacteria before and after rainfall simulations were observed only for Escherichia coli in the constant rainfall intensity experiment. Differences in flow-weighted concentrations were observed only for testosterone in both constant and variable intensity rainfall experiments, and were greatest for treatments that received poultry litter. Total loads of E. coli and fecal enterococci, were largest for both tillage treatments receiving poultry litter for the variable rainfall intensity. Load of testosterone was greatest for no-till plots receiving poultry litter under variable rainfall intensity. Poultry litter application rates commensurate for corn appeared to enhance only soil concentrations of E. coli, and runoff concentrations of testosterone above background levels.

Comparative die-off of Escherichia coli O157:H7 and fecal indicator bacteria in pond water.

In situ and in vitro experiments were performed to assess the effects of solar radiation and predation by indigenous microflora on the relative die-off rates of a toxigenic strain of Escherichia coli O157:H7, commensal E. coli, and fecal enterococci in surface waters from ponds in agricultural watersheds. The objective of these experiments was to discern a mechanism of persistence of E. coli O157:H7 in surface waters compared to fecal indicator bacteria. Results of these experiments indicated that E. coli and fecal enterococci were affected by both insolation and apparent predation; whereas E. coli O157:H7 appeared to be resistant to both of these environmental stressors. The number of days to reach 99% die-off (T(99)-values) for E. coli O157:H7 was significantly greater than that for the indicator bacteria. The capacity to prolong die-off may be connected to the apparent persistence of E. coli O157:H7 in surface waters.

17β-Estradiol and testosterone in drainage and runoff from poultry litter applications to tilled and no-till crop land under irrigation.

Thirteen million [corrected] metric tons of poultry litter are produced annually by poultry producers in the U.S. Poultry litter contains the sex hormones estradiol and testosterone, endocrine disruptors that have been detected in surface waters. The objective of this study was to evaluate the potential impact of poultry litter applications on estradiol and testosterone concentrations in subsurface drainage and surface runoff in irrigated crop land under no-till and conventional-till management. We conducted an irrigation study in fall of 2001 and spring of 2002. Four treatments, no-till plus poultry litter, conventional-till plus poultry litter, no-till plus conventional fertilizer, and conventional-till plus conventional fertilizer, were evaluated. Flow-weighted concentration and load ha(-1) of the two hormones were measured in drainage and runoff. Soil concentrations of estradiol and testosterone were measured. Based on comparisons to the conventional fertilizer (and control) treatments, poultry litter did not add to the flow-weighted concentration or load ha(-1) of either estradiol or testosterone in subsurface drainage or surface runoff. Significant differences were, however, observed between tillage treatments: flow-weighted concentrations of estradiol were greater for no-till than conventional-till plots of the June irrigation; and runoff loads of both estradiol and testosterone were less from no-till than conventional-till plots for the November irrigation. Although the differences between no-till and conventional-tillage appeared to affect the hydrologic transport of both hormones, the differences appeared to have inconsequential environmental impact.

Most probable number methodology for quantifying dilute concentrations and fluxes of Salmonella in surface waters.

Aims:  To better understand and manage the fate and transport of Salmonella in agricultural watersheds, we developed a culture‐based, five tube–four dilution most probable number (MPN) method for enumerating dilute densities of Salmonella in environmental waters.

Evaluation of percent flow captured by a small in-field runoff collector

Increased environmental concern about nutrient loadings in runoff and surface water has heightened the need for evaluation of management practices at the field scale. Thus, there is a need for small in–field runoff collectors to assess nutrient migration at the field scale on various land management systems. A runoff sampler previously designed for the relatively flat terrain of the Coastal Plain was modified to accommodate steeper slopes (5% to 12%), larger flow rates, and channelized flow common in the Southern Piedmont. We conducted a laboratory study with the modified sampler to evaluate the effect of flow rate (1 to 5.5 L min –1 ) and slope (5% and 12%) on percent flow capture. Our results show that slope and flow rate had little to no effect on the percent flow capture of the modified runoff collector. The 95% confidence interval for percent flow capture in a single observation was 10.4 µ 2.5% for the 10x splitter and 2.0 µ 1.7% for the 100x splitter. Because of the potential errors for single observations, several runoff collectors should be used if runoff volume needs to be quantified. The use of this small in–field runoff collector would be fairly unobtrusive, inexpensive in different management systems, and could help further our knowledge of migration of nutrients, pesticides, microorganisms, and sediments from fields into streams.

Total Phosphorus, Zinc, Copper, and Manganese Concentrations in Cecil Soil Through 10 Years of Poultry Litter Application

Poultry litter (PL) is an inexpensive and effective source of plant nutrients. However, overapplication could result in phosphorus (P) and heavy metal accumulation in soils. A field experiment evaluating PL application to a Cecil soil used for cotton and corn production has been maintained for 10 years. At the end of the cotton phase (i.e., the first 5 years), PL annually applied at 4.5 Mg ha−1 did not increase concentrations of total soil P, zinc (Zn), Cu, or manganese. During the corn phase (i.e. the second 5 years), PL application rates were increased from two to four times that used for cotton partly because of corns greater N demand. With this change, the average total P in the surface 15-cm soil nearly doubled to about 560 mg kg−1 of dry soil in both conventional till and no-till fields at the end of the corn phase. During the same time, Cu increased from 7 to 22 mg kg−1 and Zn increased from 17 to 32 mg kg−1 of dry soil. Levels of manganese were basically unchanged. Total P and Cu also increased in the 15- to 30-cm depth, with concentrations in the 0 to 15 cm being 1.8 to two times that in the 15 to 30 cm for P and approximately two times for Cu. Relationships between extractable versus total P and Zn changed at a threshold point beyond which extractable P and Zn increased at more than double the initial rate. It seems that once accumulation of P and Zn exceeded the soil buffer capacity, nutrient availability was significantly altered. Therefore, close monitoring of soil nutrients especially P is essential to avoid over application of PL that may potentially pose environmental risks for water pollution.

Flue gas desulfurization gypsum: implication for runoff and nutrient losses associated with broiler litter use on pastures on ultisols.

Gypsum has been shown to reduce runoff on soils prone to crust formation in the southeastern United States. Increased infiltration from gypsum applications could therefore help reduce runoff P and other nutrient losses from application of broiler litter (BL), a nutrient-rich fertilizer. In rainfall simulation experiments in June 2009 and May 2011, runoff and nutrient (N, P, Ca, Mg) losses in runoff were compared among treatments consisting of 0, 2.2, 4.5, and 9.0 Mg ha flue gas desulfurization gypsum (FGDG) combined with 13.5 Mg ha of BL and two control treatments of (0-0) and (9.0-0.0) Mg ha (FGDG-BL). A randomized block design with three replications was set up on a Cecil (Typic Kanhapludult) soil growing Coastal bermudagrass ( L.) for hay near Watkinsville, Georgia. Amendments were applied each April from 2009 to 2011. A reduction in runoff of 30% each year from the (9.0-13.5) compared with the (0-0) treatment was not statistically significant. Gypsum was effective in reducing concentration and load in one of the two study years: P and NH-N in 2009 (up to 83%) and NO-N in 2011 (up to 73%). A combination of factors related to weather extremes, timing of FGDG and BL applications, and their implication on soil and vegetation responses at different landscape positions might have caused the different responses between years. Additional studies focused on isolating the impacts of such factors would be helpful to ascertain the effectiveness of multiyear applications of FGDG as a best management practice to reduce P and other nutrient losses in soils of the southeastern United States.